FastQCFastQC Report
Thu 26 May 2016
SRR1926912_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926912_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences666002
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT11610.17432380082942694No Hit
GTCCTACAGTGTGCATTTCTCATTT9170.13768727421238977No Hit
CTGTAGGACGTGGAATATGGCAAGA9100.1366362263176387No Hit
GTATCAACGCAGAGTACTTTTTTTT8480.12732694496412925No Hit
GTCCTAAAGTGTGTATTTCTCATTT8470.12717679526487907No Hit
GTACATGGGAAGCAGTGGTATCAAC7310.1097594301518614No Hit
CTTTAGGACGTGAAATATGGCGAGG6990.10495463977585653No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAACGA601.4936177E-615.80266919
TCCAACG1055.456968E-1215.35116318
TGCCGGT759.862033E-713.90634910
CCCCTAT500.001450042813.3575011
CTAGAGC603.9631294E-412.7127823
TAGGACC3600.012.697444
AGACAGT1301.891749E-1012.4495046
GTCCTAG1001.3590034E-712.4033941
CCAACGT550.003110104212.067492519
GGACCTG4800.011.9002616
CTGTAGG6900.011.891771
TGTAGGA7850.011.6592512
CGGTTTC907.60488E-611.58862413
GTAGGAC7500.011.44150453
TCCTAGG1001.843262E-611.44063952
ACTGTTC3200.011.2967298
GACCTGG4750.011.2238247
AGGACCT5350.011.2107125
GCCGGTT855.4262608E-511.15314911
GATCTAC600.00568411211.131251
Produced by FastQC (version 0.11.5)