##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926912_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 666002 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.424738964747853 31.0 28.0 31.0 15.0 34.0 2 27.633280380539397 31.0 28.0 31.0 15.0 34.0 3 27.07908084360107 31.0 27.0 31.0 10.0 34.0 4 30.72175008483458 35.0 32.0 37.0 12.0 37.0 5 30.625384007855832 35.0 31.0 37.0 10.0 37.0 6 30.374737613400562 35.0 30.0 37.0 10.0 37.0 7 30.486690730658466 35.0 31.0 37.0 10.0 37.0 8 30.06470701289185 35.0 30.0 37.0 10.0 37.0 9 32.2488385920763 37.0 32.0 39.0 10.0 39.0 10 32.42758280005165 37.0 32.0 39.0 10.0 39.0 11 32.57285864006414 37.0 33.0 39.0 10.0 39.0 12 32.4586712952814 37.0 32.0 39.0 10.0 39.0 13 32.559240062342155 37.0 33.0 39.0 10.0 39.0 14 33.51576271542728 39.0 33.0 40.0 10.0 41.0 15 33.077367635532624 38.0 32.0 40.0 8.0 41.0 16 33.15162867378777 38.0 32.0 40.0 2.0 41.0 17 33.36841330806814 38.0 33.0 40.0 2.0 41.0 18 33.40490869396788 39.0 33.0 40.0 2.0 41.0 19 32.773593772991674 39.0 32.0 40.0 2.0 41.0 20 33.06778358022949 39.0 32.0 40.0 2.0 41.0 21 33.044031399305105 39.0 32.0 40.0 2.0 41.0 22 33.06343374344221 39.0 33.0 40.0 2.0 41.0 23 33.035304698784685 39.0 33.0 40.0 2.0 41.0 24 31.722700532430835 38.0 31.0 40.0 2.0 41.0 25 32.28187603040231 38.0 32.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.5388341065104498 1101 2 -0.6376979080296259 1101 3 -1.480141523659313 1101 4 -0.79486095248593 1101 5 -0.6870810515472137 1101 6 -2.696626446366306 1101 7 -0.9967109298186188 1101 8 -0.2781329197313447 1101 9 -0.3704150006165392 1101 10 -8.60485195090623 1101 11 -3.8488480784327734 1101 12 -1.6769021446656325 1101 13 -1.0299109625488398 1101 14 -0.859030512635826 1101 15 -1.853995801836266 1101 16 -0.688559252683568 1101 17 -0.4222178432994568 1101 18 -0.10588147502387102 1101 19 -0.785389464909759 1101 20 -0.11953487593012824 1101 21 -0.09267658705415016 1101 22 0.1733710098649155 1101 23 -0.04123886261574938 1101 24 -0.5144268137207604 1101 25 -0.1419463204100282 1102 1 0.18585050565297223 1102 2 -0.014234191352183956 1102 3 -0.2524626073819256 1102 4 -0.3134427134538669 1102 5 0.0016475177324046797 1102 6 -0.6598938765500293 1102 7 0.22377596476480122 1102 8 0.7338117720362476 1102 9 0.5204809325777475 1102 10 0.6583703512725165 1102 11 0.47855763046313626 1102 12 0.4757179222539136 1102 13 0.4736078021634995 1102 14 0.3068474303402695 1102 15 0.7949001882762303 1102 16 0.6576355620680019 1102 17 0.39778849901475866 1102 18 0.329516362897472 1102 19 0.4785524459969892 1102 20 0.40639136620804805 1102 21 0.515447673517393 1102 22 0.49180397038400514 1102 23 0.1061955493175546 1102 24 -3.077132379914598 1102 25 -0.9757004418978674 1103 1 -0.04213124674596003 1103 2 -0.0668124515378068 1103 3 0.12534453435159776 1103 4 0.037433486011291706 1103 5 -0.3391736243157304 1103 6 0.7852657899593289 1103 7 0.5027660544297383 1103 8 0.660333716349605 1103 9 0.6345001240202564 1103 10 0.4758943405756497 1103 11 0.06207815901434088 1103 12 0.23123467543938148 1103 13 0.4754986311708933 1103 14 0.2795074901168917 1103 15 0.7071481039597316 1103 16 0.6538192965339462 1103 17 0.2779269290918407 1103 18 0.2372464242471679 1103 19 -1.4039172724236515 1103 20 -0.3502296814559429 1103 21 -0.039851996138104084 1103 22 0.202098134297799 1103 23 -0.04842140836688458 1103 24 1.4425655908263124 1103 25 0.36355706950688216 1104 1 -0.5462250666672617 1104 2 -0.5267677019042907 1104 3 -1.5463032294526364 1104 4 -0.5290835008467205 1104 5 -0.6019259839587612 1104 6 -1.5975678391330739 1104 7 -0.2203201271920534 1104 8 0.22033165889519069 1104 9 -0.035923454407338795 1104 10 0.08410358368960402 1104 11 0.006569067637641979 1104 12 -0.07558527158116846 1104 13 -0.37093502878332885 1104 14 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7 0.6182400178528837 2215 8 -0.3410530396651019 2215 9 0.03532659337666644 2215 10 -0.208735004670757 2215 11 0.0055897230703649825 2215 12 -0.37968905297981337 2215 13 0.1119619379790251 2215 14 0.11277567383879727 2215 15 0.14433364074599098 2215 16 0.3253265793297899 2215 17 -0.13873182655058258 2215 18 -0.7051371208223998 2215 19 0.4817553973143518 2215 20 -3.6240125471081406 2215 21 -1.9105262946163961 2215 22 -0.8970539900448884 2215 23 -0.5410548487317257 2215 24 -1.1057121326601198 2215 25 -0.6363880186777706 2216 1 -1.3705595810016327 2216 2 -1.793505866036977 2216 3 -0.9804311527909952 2216 4 -1.440533678718463 2216 5 -1.0944909089495596 2216 6 -2.7324689453293622 2216 7 -10.317607492886257 2216 8 -11.446371688557239 2216 9 -7.058538060816492 2216 10 -4.9889645415494215 2216 11 -3.892832374097633 2216 12 -3.2719630740533354 2216 13 -3.1028420808748542 2216 14 -3.4662451385494073 2216 15 -4.232135238596996 2216 16 -3.540927060226803 2216 17 -3.712249237667063 2216 18 -3.397981393498636 2216 19 -2.9579977003889724 2216 20 -3.7670079511478853 2216 21 -6.289138105711984 2216 22 -5.114890428697954 2216 23 -4.955043802759064 2216 24 -5.3129057244291715 2216 25 -4.491585893046153 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 49096.0 3 15.0 4 366.0 5 2083.0 6 2624.0 7 2064.0 8 1586.0 9 1538.0 10 1476.0 11 1488.0 12 1420.0 13 1368.0 14 1407.0 15 1468.0 16 1509.0 17 1692.0 18 1964.0 19 2422.0 20 3007.0 21 4062.0 22 5009.0 23 5802.0 24 6912.0 25 7707.0 26 8674.0 27 9531.0 28 10474.0 29 12631.0 30 14657.0 31 17984.0 32 23040.0 33 29378.0 34 39651.0 35 54491.0 36 77770.0 37 119140.0 38 140496.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.67855530474041 19.18043641835967 12.922799097065463 25.21820917983446 2 13.643395934224712 20.115529240895906 43.40706015368817 22.83401467119121 3 18.78470316716909 25.495222558844095 29.441216048593834 26.27885822539298 4 11.792238077565132 17.134889283895117 39.44729847294867 31.625574165591086 5 12.269819790729562 38.3263471390145 36.427233692330105 12.97659937792583 6 31.324548466453 37.86273620468519 18.639707317952308 12.173008010909495 7 26.958712736188634 31.4668168715417 23.38210975598736 18.19236063628231 8 23.832035385702042 38.01854526451174 21.228409164499237 16.92101018528698 9 25.79786714804263 15.85446987320329 20.60795742105429 37.739705557699786 10 13.9539808972208 30.038286667858277 35.34735125051257 20.660381184408358 11 33.76458768529796 23.225862646364924 24.678811870482527 18.33073779785459 12 22.44959256556045 25.28976953597828 32.765808546361626 19.494829352099647 13 28.50955639753773 22.57703611262538 25.96814183745339 22.945265652383505 14 19.953054110919048 23.0440339223846 28.288577551575152 28.7143344151212 15 22.79820991455045 31.740527714787724 23.441259066812837 22.020003303848984 16 21.087141964628987 28.99605920799692 28.92607410611303 20.990724721261053 17 20.99879554622429 29.321841462645416 28.441629046258832 21.237733944871458 18 20.77024155459945 28.66983533439957 30.26032632772434 20.299596783276645 19 23.200111739730534 27.487222772076482 27.613080156735997 21.699585331456987 20 22.71255522816629 28.47130237852807 28.55705433760741 20.259088055698225 21 22.324291285784867 27.550687877408997 27.556544709403518 22.568476127402615 22 22.052436504313143 28.94970805199798 27.79077660571401 21.207078837974866 23 21.62125226020773 28.611226249046357 28.026275478022672 21.74124601272324 24 22.76705531827984 27.86983474797715 28.41106365596823 20.952046277774773 25 22.1038515196886 28.027926408597175 28.373477420546543 21.49474465116768 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 223.0 1 223.0 2 284.0 3 345.0 4 345.0 5 345.0 6 494.5 7 644.0 8 644.0 9 644.0 10 1072.0 11 1500.0 12 1500.0 13 1500.0 14 2560.0 15 3620.0 16 3620.0 17 3620.0 18 6306.0 19 8992.0 20 8992.0 21 8992.0 22 13911.0 23 18830.0 24 18830.0 25 18830.0 26 26828.5 27 34827.0 28 34827.0 29 34827.0 30 45432.0 31 56037.0 32 56037.0 33 56037.0 34 62895.5 35 69754.0 36 69754.0 37 69754.0 38 74662.5 39 79571.0 40 79571.0 41 79571.0 42 82663.5 43 85756.0 44 85756.0 45 85756.0 46 86616.5 47 87477.0 48 87477.0 49 87477.0 50 82454.5 51 77432.0 52 77432.0 53 77432.0 54 67239.0 55 57046.0 56 57046.0 57 57046.0 58 47918.5 59 38791.0 60 38791.0 61 38791.0 62 31200.0 63 23609.0 64 23609.0 65 23609.0 66 18061.0 67 12513.0 68 12513.0 69 12513.0 70 9062.5 71 5612.0 72 5612.0 73 5612.0 74 3910.0 75 2208.0 76 2208.0 77 2208.0 78 1518.0 79 828.0 80 828.0 81 828.0 82 544.0 83 260.0 84 260.0 85 260.0 86 172.5 87 85.0 88 85.0 89 85.0 90 57.5 91 30.0 92 30.0 93 30.0 94 19.5 95 9.0 96 9.0 97 9.0 98 6.0 99 3.0 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2255248482737289 2 0.13348308263338549 3 0.1346842802273867 4 0.07072050834682178 5 0.02477470037627515 6 0.024023951880024385 7 0.3033023924853078 8 0.3010501469965556 9 0.01996991000027027 10 0.03528517932378582 11 0.017267215413767526 12 0.01921916150401951 13 0.01696691601526722 14 0.019369311203269658 15 0.01681676631601707 16 0.02192185609052225 17 0.021020957895021338 18 0.016066017819766305 19 0.025375299173275755 20 0.01921916150401951 21 0.01696691601526722 22 0.01951946090251981 23 0.02012005969952042 24 0.016516466917516764 25 0.01636631721826661 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 666002.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 86.92316669698084 #Duplication Level Percentage of deduplicated Percentage of total 1 94.29393622971182 81.96327537409721 2 4.362773124909477 7.58452111195229 3 0.6722502533072221 1.7530236249093378 4 0.226297625617767 0.7868202493881651 5 0.12088216362625033 0.5253730229788135 6 0.06906751005454939 0.3602140013890198 7 0.04337853472950184 0.2639419723754282 8 0.02856893978527831 0.19866421722494038 9 0.023244079694472657 0.18184041126004985 >10 0.13124413680843036 2.185705663554488 >50 0.015216388643990785 0.8981987143504072 >100 0.011066116306313835 1.9511578281252333 >500 0.001901988737874497 1.1727687970664598 >1k 1.7290806707949972E-4 0.1744950113282276 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 1161 0.17432380082942694 No Hit GTCCTACAGTGTGCATTTCTCATTT 917 0.13768727421238977 No Hit CTGTAGGACGTGGAATATGGCAAGA 910 0.1366362263176387 No Hit GTATCAACGCAGAGTACTTTTTTTT 848 0.12732694496412925 No Hit GTCCTAAAGTGTGTATTTCTCATTT 847 0.12717679526487907 No Hit GTACATGGGAAGCAGTGGTATCAAC 731 0.1097594301518614 No Hit CTTTAGGACGTGAAATATGGCGAGG 699 0.10495463977585653 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 3.002993985003048E-4 0.0 0.0 0.0 6 0.0 3.002993985003048E-4 0.0 0.0 0.0 7 0.0 3.002993985003048E-4 0.0 0.0 0.0 8 0.0 3.002993985003048E-4 0.0 0.0 0.0 9 0.0 3.002993985003048E-4 0.0 0.0 0.0 10 0.0 3.002993985003048E-4 0.0 0.0 0.0 11 0.0 3.002993985003048E-4 0.0 0.0 0.0 12 0.0 3.002993985003048E-4 0.0 0.0 1.501496992501524E-4 13 0.0 3.002993985003048E-4 0.0 0.0 1.501496992501524E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCAACGA 60 1.4936177E-6 15.802669 19 TCCAACG 105 5.456968E-12 15.351163 18 TGCCGGT 75 9.862033E-7 13.906349 10 CCCCTAT 50 0.0014500428 13.357501 1 CTAGAGC 60 3.9631294E-4 12.712782 3 TAGGACC 360 0.0 12.69744 4 AGACAGT 130 1.891749E-10 12.449504 6 GTCCTAG 100 1.3590034E-7 12.403394 1 CCAACGT 55 0.0031101042 12.0674925 19 GGACCTG 480 0.0 11.900261 6 CTGTAGG 690 0.0 11.89177 1 TGTAGGA 785 0.0 11.659251 2 CGGTTTC 90 7.60488E-6 11.588624 13 GTAGGAC 750 0.0 11.4415045 3 TCCTAGG 100 1.843262E-6 11.4406395 2 ACTGTTC 320 0.0 11.296729 8 GACCTGG 475 0.0 11.223824 7 AGGACCT 535 0.0 11.210712 5 GCCGGTT 85 5.4262608E-5 11.153149 11 GATCTAC 60 0.005684112 11.13125 1 >>END_MODULE