FastQCFastQC Report
Thu 26 May 2016
SRR1926912_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926912_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences666002
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT14440.2168161657172201No Hit
GTCCTACAGTGGACATTTCTAAATT11610.17432380082942694No Hit
CTGTAGGACGTGGAATATGGCAAGA10550.1584079327089108No Hit
GTCCTACAGTGTGCATTTCTCATTT9500.1426422142876448No Hit
CTTTAGGACGTGAAATATGGCGAGG9210.13828787300939036No Hit
GTCCTAAAGTGTGTATTTCTCATTT9050.13588547782138793No Hit
GGTATCAACGCAGAGTACTTTTTTT8920.13393353173113592No Hit
GTACATGGGAAGCAGTGGTATCAAC8720.1309305377461329No Hit
CTGTAGGACCTGGAATATGGCGAGA8420.12642604676862831No Hit
CCCATGTACTCTGCGTTGATACCAC7550.11336302293386505No Hit
TATCAACGCAGAGTACTTTTTTTTT7530.11306272353536476No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACTCAT350.002184602216.2676287
ACTAGAC601.4294365E-615.8693753
CTATTGG500.001459191713.3463641
TAGGAGT655.3321884E-513.1827964
TAGGACC3650.012.7814924
TAGACAG1207.1122486E-1012.6945435
CTAAGCT751.4425861E-512.6945434
TGTAGGA8250.012.3641642
AGGACCT5100.012.3211755
TCCAACG853.989626E-612.2786218
GACGTGG2650.012.1751427
CTAGTAC550.003013504212.1184313
TAAGCCT550.003015205712.1175184
TAGGACA2850.012.0264094
GTAGGAC8050.011.9463863
GGACCTG4950.011.9251776
TAAGGCT802.7969292E-511.90113454
CTGTAGG7900.011.82589151
TTAGGGT657.8663765E-411.718044
GATATAC2800.011.5759281