FastQCFastQC Report
Thu 26 May 2016
SRR1926911_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926911_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences644210
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT12630.19605408174353084No Hit
CTGTAGGACGTGGAATATGGCAAGA10530.16345601589543782No Hit
GTCCTAAAGTGTGTATTTCTCATTT9610.14917495847627327No Hit
GTCCTACAGTGTGCATTTCTCATTT8970.13924030983685443No Hit
GTATCAACGCAGAGTACTTTTTTTT8650.13427298551714503No Hit
CTTTAGGACGTGAAATATGGCGAGG8170.1268219990375809No Hit
CTGTAGGACCTGGAATATGGCGAGA7270.1128513993883982No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCCGT402.7947754E-416.59612810
AGCGTCG350.002125179616.3347933
TCCAACG704.5122033E-714.90264518
CGTTCAG456.8443245E-414.75211310
GTACAAG400.005133006314.3085711
CCCGTTT551.9864192E-413.79418412
CCAACGA551.9864192E-413.79418419
GGACCCT500.001476385513.3255296
TAGGACC2300.012.8349254
GTACTAA550.002972322512.1406061
CTAATTC550.002996758612.1273463
TATAGTG1353.583409E-1011.9859255
GGACCTG4050.011.9859246
GATATAC2100.011.810251
AGGACCT3950.011.8074295
CGTTTCC658.1299094E-411.67200214
GTCCTAA3200.011.6257141
GTGTTAA1156.654045E-811.6127531
ACTGTTC2300.011.163148
GGTCTAG600.00569275811.1288881
Produced by FastQC (version 0.11.5)