##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926911_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 644210 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.929696837987613 31.0 28.0 31.0 16.0 34.0 2 28.137144719889477 31.0 28.0 31.0 16.0 34.0 3 27.598891665761165 31.0 27.0 31.0 16.0 34.0 4 31.291069682246473 35.0 32.0 37.0 17.0 37.0 5 31.193761351112215 35.0 32.0 37.0 17.0 37.0 6 30.96000527778209 35.0 32.0 37.0 11.0 37.0 7 31.04954129864485 35.0 32.0 37.0 11.0 37.0 8 30.6615420437435 35.0 31.0 37.0 10.0 37.0 9 32.862659691715436 37.0 33.0 39.0 17.0 39.0 10 33.038524704677044 37.0 33.0 39.0 17.0 39.0 11 33.18648422098384 37.0 33.0 39.0 17.0 39.0 12 33.07102031946105 38.0 33.0 39.0 17.0 39.0 13 33.18167057326027 38.0 33.0 39.0 17.0 39.0 14 34.177123919218886 39.0 34.0 41.0 17.0 41.0 15 33.751919405162916 39.0 33.0 41.0 10.0 41.0 16 33.83136244392357 39.0 33.0 40.0 11.0 41.0 17 34.05147234597414 39.0 34.0 41.0 16.0 41.0 18 34.102269446298564 39.0 34.0 41.0 11.0 41.0 19 33.48934353704537 39.0 33.0 41.0 10.0 41.0 20 33.76819360146536 39.0 33.0 41.0 10.0 41.0 21 33.74300305800904 39.0 34.0 41.0 7.0 41.0 22 33.756743918908434 39.0 34.0 41.0 2.0 41.0 23 33.746557799475326 39.0 34.0 41.0 2.0 41.0 24 32.452933049781905 38.0 32.0 40.0 2.0 41.0 25 33.02495770012884 39.0 33.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.3811229599870387 1101 2 -0.5836656743806437 1101 3 -1.4489786113925227 1101 4 -1.0781893528923838 1101 5 -0.7471376997809571 1101 6 -2.3633492512958902 1101 7 -0.8463951973580635 1101 8 -0.5747257616780779 1101 9 -0.7734247030330721 1101 10 -9.351568195255016 1101 11 -4.108019835557844 1101 12 -1.9653873962885662 1101 13 -1.292754427381933 1101 14 -0.9986545714542672 1101 15 -2.1369170492980984 1101 16 -0.7865138297904082 1101 17 -0.8089543687184673 1101 18 -0.7988422314418671 1101 19 -1.1075409784850194 1101 20 -0.8162805607483392 1101 21 -0.8251343363437655 1101 22 -0.5738204194782099 1101 23 -0.356924294422555 1101 24 -0.4725883726226243 1101 25 -0.4447851618568066 1102 1 0.012880958181057167 1102 2 -0.11744435141011422 1102 3 -0.43754419788661636 1102 4 -0.36918398949835307 1102 5 0.013744851524563728 1102 6 -0.9841230359679329 1102 7 0.4234873646528321 1102 8 0.45965134594307955 1102 9 0.2485213204545218 1102 10 0.3652478114251352 1102 11 0.16561496120056773 1102 12 0.2605174757286477 1102 13 0.30603051229326184 1102 14 -0.13096125910347212 1102 15 0.3513177410370858 1102 16 0.17282286503140654 1102 17 -0.007839938940385593 1102 18 -0.2712309222812763 1102 19 0.1732439397794252 1102 20 0.05596681911056578 1102 21 0.14534344464562565 1102 22 -0.09090299005340796 1102 23 -0.3370540435955647 1102 24 -3.822819073503677 1102 25 -1.8551822243012737 1103 1 -0.4052416679719535 1103 2 -0.38051543117137143 1103 3 -0.19878131128868048 1103 4 -0.20203260588467842 1103 5 -0.5582247632339801 1103 6 0.19108043663109342 1103 7 0.13387260610698348 1103 8 0.3433952417651085 1103 9 -0.08676896712008642 1103 10 -0.08556065303498173 1103 11 -0.2574235629448509 1103 12 -0.20368219871519244 1103 13 -0.2438338393073849 1103 14 -0.14761888254351874 1103 15 0.1264452505324769 1103 16 0.058563505291850504 1103 17 -0.05572924984652161 1103 18 -0.3408239770832324 1103 19 -1.9339617031939902 1103 20 -0.5259105547364271 1103 21 -0.6700553616484655 1103 22 -0.2454879059513999 1103 23 -0.32317449937418985 1103 24 1.2826611218300172 1103 25 0.3842534783574365 1104 1 -0.41233359634511046 1104 2 -0.23945844537700012 1104 3 -1.3771267094272517 1104 4 -0.716992601530464 1104 5 -0.30523722717563473 1104 6 -1.3575289358176086 1104 7 -0.1697598981111632 1104 8 0.0991379076335015 1104 9 0.11446871861441821 1104 10 0.24545442343593038 1104 11 0.0984660576941323 1104 12 0.3505397574160867 1104 13 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0.6645724292468635 2215 2 0.3928774172124001 2215 3 0.7995970670896995 2215 4 0.9237907128968992 2215 5 1.0830005273924002 2215 6 1.4789403473585132 2215 7 0.9997596910140345 2215 8 -0.2786217298344411 2215 9 0.21987055806753375 2215 10 0.30670206340196415 2215 11 0.20882649836873668 2215 12 -0.1673512034731246 2215 13 0.19923772590573918 2215 14 0.22406895774686575 2215 15 0.1964143484870533 2215 16 0.450235649305192 2215 17 0.10702348473924417 2215 18 -0.5675645470542889 2215 19 0.6133684581833592 2215 20 -3.186964167332686 2215 21 -1.9363073849966739 2215 22 -0.6732359807049235 2215 23 -0.7370127296189537 2215 24 -0.6046101785084339 2215 25 -0.13369030935042048 2216 1 -0.22832008223966582 2216 2 -0.7458774550155134 2216 3 0.17352071449401052 2216 4 -0.17139773140566206 2216 5 0.17392217870650484 2216 6 -1.5014076897622388 2216 7 -9.62202154917071 2216 8 -10.694887204682502 2216 9 -5.791396281261793 2216 10 -3.377703717879413 2216 11 -2.1605892118737984 2216 12 -1.925172942148361 2216 13 -1.763810576731057 2216 14 -2.118821751594595 2216 15 -2.8860234903805804 2216 16 -1.8001337904336552 2216 17 -2.1908324775289856 2216 18 -2.141343507954609 2216 19 -1.645629823771678 2216 20 -2.201727168093022 2216 21 -4.979474766514219 2216 22 -3.4924104609577 2216 23 -3.14780569061195 2216 24 -3.7517287201783205 2216 25 -3.351582520479436 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 40739.0 3 10.0 4 306.0 5 1715.0 6 2276.0 7 1771.0 8 1469.0 9 1404.0 10 1259.0 11 1228.0 12 1189.0 13 1211.0 14 1237.0 15 1241.0 16 1352.0 17 1460.0 18 1741.0 19 2145.0 20 2730.0 21 3572.0 22 4377.0 23 5246.0 24 6008.0 25 6936.0 26 7823.0 27 8504.0 28 9630.0 29 11224.0 30 13545.0 31 16705.0 32 21182.0 33 27918.0 34 37270.0 35 51659.0 36 75056.0 37 117748.0 38 153324.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.58062809876213 19.027002303993328 13.0386420596238 25.353727537620742 2 13.728803021589442 19.84892052784556 43.419494225717706 23.002782224847287 3 18.757713386864626 25.21768460058256 29.451226525657404 26.5733754868954 4 11.948095929013276 17.044674286886035 39.128138369430474 31.879091414670217 5 12.531596435114741 38.02611557929386 36.29754991770953 13.144738067881875 6 31.475549828045306 37.65318717831275 18.587875447353916 12.28338754628803 7 27.300384253694627 31.229175878583305 23.274906427686197 18.19553344003587 8 24.015855914206178 37.96653816691681 20.931550529049456 17.086055389827553 9 25.744853948075257 15.936225907720397 20.614793676620824 37.70412646758352 10 14.2744008012609 29.791998260829054 35.094450957708645 20.8391499802014 11 33.72618184471642 23.013077182234408 24.859532463177246 18.401208509871932 12 22.861951398247456 25.116287735292474 32.39133539929078 19.630425467169285 13 28.515736589122255 22.60690820116006 25.83666612845131 23.04068908126638 14 20.07197673642324 23.06966186770005 28.03521829786507 28.823143098011634 15 22.65989967505399 31.889732606538974 23.253124092736144 22.19724362567089 16 21.193264211699752 28.777081179104524 28.813101437394618 21.216553171801106 17 20.96278619880172 29.344236588421786 28.40073343901231 21.292243773764184 18 20.66435444657312 28.455987604678672 30.33469230602036 20.54496564272784 19 23.290608575487738 27.357405150261236 27.586581682956933 21.765404591294086 20 22.934074840007828 28.225383251512977 28.537915273236514 20.30262663524268 21 22.368678447336308 27.413034500532525 27.34301496958575 22.87527208254542 22 22.284807591391374 28.984098693056026 27.759440333613828 20.971653381938772 23 21.63744959920011 28.507794754435352 28.143713504542088 21.711042141822457 24 22.90269042491915 27.569674848121174 28.61392425698534 20.913710469974337 25 22.146078667298035 27.908493180459708 28.522368247413077 21.42305990482918 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 184.0 1 184.0 2 236.0 3 288.0 4 288.0 5 288.0 6 464.5 7 641.0 8 641.0 9 641.0 10 1011.5 11 1382.0 12 1382.0 13 1382.0 14 2349.5 15 3317.0 16 3317.0 17 3317.0 18 5814.5 19 8312.0 20 8312.0 21 8312.0 22 13004.5 23 17697.0 24 17697.0 25 17697.0 26 25312.0 27 32927.0 28 32927.0 29 32927.0 30 42586.0 31 52245.0 32 52245.0 33 52245.0 34 59505.0 35 66765.0 36 66765.0 37 66765.0 38 72014.0 39 77263.0 40 77263.0 41 77263.0 42 79967.0 43 82671.0 44 82671.0 45 82671.0 46 83670.5 47 84670.0 48 84670.0 49 84670.0 50 80547.0 51 76424.0 52 76424.0 53 76424.0 54 66551.0 55 56678.0 56 56678.0 57 56678.0 58 47442.5 59 38207.0 60 38207.0 61 38207.0 62 30757.0 63 23307.0 64 23307.0 65 23307.0 66 17854.5 67 12402.0 68 12402.0 69 12402.0 70 8956.0 71 5510.0 72 5510.0 73 5510.0 74 3846.0 75 2182.0 76 2182.0 77 2182.0 78 1479.5 79 777.0 80 777.0 81 777.0 82 518.5 83 260.0 84 260.0 85 260.0 86 171.0 87 82.0 88 82.0 89 82.0 90 47.5 91 13.0 92 13.0 93 13.0 94 8.0 95 3.0 96 3.0 97 3.0 98 3.0 99 3.0 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2193384144921687 2 0.13039226339237206 3 0.1316340944722994 4 0.07513078033560484 5 0.023284332748637865 6 0.02250818832368327 7 0.2989708324925102 8 0.2989708324925102 9 0.01924838173887397 10 0.0349264991229568 11 0.017230406233992024 12 0.018317008428928454 13 0.016454261809037425 14 0.01754086400397386 15 0.01598857515406467 16 0.019869297278837645 17 0.01878269508390121 18 0.014901972959128235 19 0.025612766023501654 20 0.018006550658946616 21 0.01614380403905559 22 0.017385635118982938 23 0.019093152853883053 24 0.017230406233992024 25 0.016299032924046507 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 644210.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 86.56893088434401 #Duplication Level Percentage of deduplicated Percentage of total 1 94.00119345059224 81.3758281887017 2 4.577324482637876 7.925081735453879 3 0.7015076489507165 1.8218630153055975 4 0.24237629139324787 0.8392902567050279 5 0.11810001967893256 0.5111896220512591 6 0.07719618269384122 0.40096746024950003 7 0.05063989514887517 0.30686891081933815 8 0.03265661621397865 0.22616386815555717 9 0.02576466645039533 0.20073776662822052 >10 0.1450617441879257 2.3231702506916507 >50 0.015256291659385015 0.9145563251302367 >100 0.011486853683180313 2.04862893881984 >500 0.0010768925321378014 0.7458034814760283 >1k 3.589641773792671E-4 0.3598501798121836 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 1263 0.19605408174353084 No Hit CTGTAGGACGTGGAATATGGCAAGA 1053 0.16345601589543782 No Hit GTCCTAAAGTGTGTATTTCTCATTT 961 0.14917495847627327 No Hit GTCCTACAGTGTGCATTTCTCATTT 897 0.13924030983685443 No Hit GTATCAACGCAGAGTACTTTTTTTT 865 0.13427298551714503 No Hit CTTTAGGACGTGAAATATGGCGAGG 817 0.1268219990375809 No Hit CTGTAGGACCTGGAATATGGCGAGA 727 0.1128513993883982 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 1.552288849909191E-4 0.0 5 0.0 1.552288849909191E-4 0.0 1.552288849909191E-4 0.0 6 0.0 1.552288849909191E-4 0.0 1.552288849909191E-4 0.0 7 0.0 1.552288849909191E-4 0.0 1.552288849909191E-4 0.0 8 0.0 1.552288849909191E-4 0.0 1.552288849909191E-4 0.0 9 0.0 1.552288849909191E-4 0.0 1.552288849909191E-4 0.0 10 0.0 1.552288849909191E-4 0.0 1.552288849909191E-4 0.0 11 0.0 1.552288849909191E-4 0.0 1.552288849909191E-4 0.0 12 0.0 1.552288849909191E-4 0.0 1.552288849909191E-4 1.552288849909191E-4 13 0.0 1.552288849909191E-4 0.0 1.552288849909191E-4 1.552288849909191E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATCCCGT 40 2.7947754E-4 16.596128 10 AGCGTCG 35 0.0021251796 16.334793 3 TCCAACG 70 4.5122033E-7 14.902645 18 CGTTCAG 45 6.8443245E-4 14.752113 10 GTACAAG 40 0.0051330063 14.308571 1 CCCGTTT 55 1.9864192E-4 13.794184 12 CCAACGA 55 1.9864192E-4 13.794184 19 GGACCCT 50 0.0014763855 13.325529 6 TAGGACC 230 0.0 12.834925 4 GTACTAA 55 0.0029723225 12.140606 1 CTAATTC 55 0.0029967586 12.127346 3 TATAGTG 135 3.583409E-10 11.985925 5 GGACCTG 405 0.0 11.985924 6 GATATAC 210 0.0 11.81025 1 AGGACCT 395 0.0 11.807429 5 CGTTTCC 65 8.1299094E-4 11.672002 14 GTCCTAA 320 0.0 11.625714 1 GTGTTAA 115 6.654045E-8 11.612753 1 ACTGTTC 230 0.0 11.16314 8 GGTCTAG 60 0.005692758 11.128888 1 >>END_MODULE