FastQCFastQC Report
Thu 26 May 2016
SRR1926911_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926911_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences644210
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT14890.23113580975147857No Hit
GTCCTACAGTGGACATTTCTAAATT14020.2176308967572686No Hit
CTGTAGGACGTGGAATATGGCAAGA12580.19527793731857623No Hit
GTCCTACAGTGTGCATTTCTCATTT10420.1617484981605377No Hit
GTCCTAAAGTGTGTATTTCTCATTT10270.15942006488567392No Hit
GGTATCAACGCAGAGTACTTTTTTT9860.15305568060104624No Hit
CTTTAGGACGTGAAATATGGCGAGG9360.1452942363515003No Hit
CTGTAGGACCTGGAATATGGCGAGA8970.13924030983685443No Hit
TATCAACGCAGAGTACTTTTTTTTT7550.11719780816814393No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCACCG250.005934749719.051525
TGCGCTG250.006075055318.97461510
CCAACGA555.9795275E-717.24965119
GGCTTAG500.001459874313.3454251
TCCAACG905.476577E-712.64974518
TAGGACC3250.012.3102134
CCTAATG550.002991274712.1303132
TAGTACT550.003003531112.1236954
CGTTGAT1157.183553E-811.54886914
GTCTAGG752.0056576E-411.4389351
GTATAGC1001.846267E-611.4389351
TCTACAC1751.8189894E-1211.4326943
GGCGAGG2250.011.384768519
TGTAGAA2350.011.3560372
ATTAACC855.158629E-511.2085243
TACACTC855.1668314E-511.2067775
GTGTTAG600.00572218611.1211881
GTGCTAG600.00572218611.1211881
TCATGAC600.005745491511.1151193
GGTATCA4550.011.1037291
Produced by FastQC (version 0.11.5)