##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926911_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 644210 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.787282097452692 31.0 30.0 33.0 25.0 34.0 2 30.117671256267368 31.0 30.0 34.0 26.0 34.0 3 30.164896539948153 31.0 30.0 34.0 26.0 34.0 4 33.752915974604555 35.0 35.0 37.0 28.0 37.0 5 33.68627310970025 37.0 35.0 37.0 28.0 37.0 6 33.60118284410363 37.0 35.0 37.0 28.0 37.0 7 33.59698235047578 37.0 35.0 37.0 28.0 37.0 8 33.62893932102886 37.0 35.0 37.0 28.0 37.0 9 35.16373853246612 39.0 35.0 39.0 29.0 39.0 10 35.08264385836917 39.0 35.0 39.0 29.0 39.0 11 35.08262523090297 39.0 35.0 39.0 29.0 39.0 12 34.98790146070381 39.0 35.0 39.0 28.0 39.0 13 34.99770882165753 39.0 35.0 39.0 28.0 39.0 14 36.089439778954066 40.0 36.0 41.0 28.0 41.0 15 36.061964266310675 40.0 36.0 41.0 28.0 41.0 16 36.04805886279319 40.0 36.0 41.0 27.0 41.0 17 36.02920631471104 40.0 36.0 41.0 27.0 41.0 18 36.00830940221356 40.0 36.0 41.0 27.0 41.0 19 36.07706493224259 40.0 36.0 41.0 27.0 41.0 20 36.042121357942285 40.0 36.0 41.0 27.0 41.0 21 35.99445832880583 40.0 36.0 41.0 27.0 41.0 22 35.91297092562984 40.0 36.0 41.0 27.0 41.0 23 35.823989071886494 40.0 36.0 41.0 27.0 41.0 24 35.74760869902671 40.0 36.0 41.0 27.0 41.0 25 35.63276571304388 40.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.16044525204010185 1101 2 0.03380022441564634 1101 3 0.26798675168608455 1101 4 0.25235752946143464 1101 5 0.2740484221437782 1101 6 0.12229236827732848 1101 7 0.20702024856137768 1101 8 0.019965783245858404 1101 9 0.2693835030753178 1101 10 0.23151019131328354 1101 11 0.08938200833153331 1101 12 0.30505584353540627 1101 13 0.2288481475197699 1101 14 0.2695001440215563 1101 15 0.11527007764505015 1101 16 0.11193802396195451 1101 17 0.2331928785109625 1101 18 0.2693459782620735 1101 19 0.1925598168089664 1101 20 0.2525804463716099 1101 21 0.24953140474245572 1101 22 0.05698839405894773 1101 23 0.14664747522508037 1101 24 0.0906923379397071 1101 25 0.3470832177065333 1102 1 -0.30290274766014846 1102 2 -0.12185810276209352 1102 3 -0.011351870103638362 1102 4 0.027525336250782573 1102 5 -0.10296392115625963 1102 6 -0.2178399501862387 1102 7 -0.037730369674164876 1102 8 -0.018558587545591365 1102 9 -0.11805266611246168 1102 10 0.007754959060129352 1102 11 -0.21166711864494658 1102 12 -0.01734622902564098 1102 13 -0.14261131656904524 1102 14 -0.16076288709897568 1102 15 -0.14362341455608174 1102 16 -0.2817222515944593 1102 17 -0.004568198878210694 1102 18 -0.16022762655962453 1102 19 -0.08860514235001915 1102 20 -0.20922169684492786 1102 21 -0.26962800903108075 1102 22 -0.41687870938711313 1102 23 -0.0748746153210007 1102 24 -0.26057357292732064 1102 25 -0.262903828786925 1103 1 -0.7848452327388244 1103 2 -0.7653198499134781 1103 3 -0.6293876812810666 1103 4 -0.9211561613075432 1103 5 -0.7412710291323918 1103 6 -0.8670206338541746 1103 7 -0.9739864738521362 1103 8 -0.8409677031180252 1103 9 -0.9356435505400285 1103 10 -1.1487073306848572 1103 11 -1.103365436604797 1103 12 -1.05998323390898 1103 13 -1.1401587935088173 1103 14 -1.212211612004026 1103 15 -1.0993694807525003 1103 16 -0.9727151978776973 1103 17 -0.8699398754924275 1103 18 -1.257883622218877 1103 19 -0.9777424185301555 1103 20 -1.0657165422057489 1103 21 -1.151581345981704 1103 22 -1.0700094744440847 1103 23 -1.0462256733785154 1103 24 -1.1991628295740924 1103 25 -1.2040541272134035 1104 1 -0.09879396185219491 1104 2 -0.07226482351604702 1104 3 0.28175040177559296 1104 4 0.22955520863748546 1104 5 0.048785031391204825 1104 6 0.06663962848730876 1104 7 0.03422775354676588 1104 8 0.15595713223820695 1104 9 -0.03941430109791355 1104 10 -0.04891089025596784 1104 11 0.2139099130931328 1104 12 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0.34532359821003666 2214 10 0.18994979371254317 2214 11 0.41803509020798657 2214 12 0.27226466629478807 2214 13 0.28168703920327687 2214 14 0.3466492796017633 2214 15 0.14694929068032536 2214 16 0.15191578467784694 2214 17 0.12334375852179136 2214 18 0.17668523618775822 2214 19 0.33162888508298494 2214 20 0.21035522361106018 2214 21 0.11286276713394727 2214 22 0.035066814010761505 2214 23 0.32388515610311686 2214 24 0.0901687145837542 2214 25 -0.01808523898716885 2215 1 0.747287008395233 2215 2 0.4247277294262979 2215 3 0.298284855139201 2215 4 0.61454698285295 2215 5 0.6782266900023544 2215 6 0.586928451347184 2215 7 0.39436002146350546 2215 8 0.3557998448291926 2215 9 0.2794435147466743 2215 10 0.3628264898870768 2215 11 0.11921267689890414 2215 12 0.2983588468287408 2215 13 0.47612216985018563 2215 14 0.30490615912463426 2215 15 0.4687602097365371 2215 16 0.18708761764199977 2215 17 0.3277066639689963 2215 18 0.25303608151389057 2215 19 0.3349774519755542 2215 20 0.401124091531436 2215 21 0.3828945455177788 2215 22 0.35846357210518676 2215 23 0.5165839602115199 2215 24 0.6014002743646927 2215 25 0.3926673129200182 2216 1 0.047836748457260825 2216 2 0.21209606556582727 2216 3 -1.658334080428105 2216 4 -1.3540261816622987 2216 5 -0.22112951512637835 2216 6 0.006640314095811561 2216 7 -0.13923024626659242 2216 8 0.19605856420774614 2216 9 0.05167640681191443 2216 10 0.035477994506578625 2216 11 -0.05968025680446942 2216 12 0.11960579390918014 2216 13 -0.0732866407512276 2216 14 -0.0485322478477741 2216 15 0.03258360638523072 2216 16 -0.009557303140852014 2216 17 0.07056802409078955 2216 18 0.13892940482386962 2216 19 0.25112450014775334 2216 20 0.1998819844977504 2216 21 0.2791407631585514 2216 22 0.13353612989826047 2216 23 0.07590285235571059 2216 24 -0.13550285089812064 2216 25 0.13759170006196797 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 16461.0 3 21.0 4 286.0 5 1915.0 6 2540.0 7 1833.0 8 1376.0 9 1223.0 10 1047.0 11 953.0 12 903.0 13 830.0 14 836.0 15 828.0 16 868.0 17 917.0 18 1012.0 19 1205.0 20 1604.0 21 1987.0 22 2698.0 23 3176.0 24 3660.0 25 4533.0 26 5261.0 27 5825.0 28 6595.0 29 8150.0 30 10528.0 31 13314.0 32 17267.0 33 23168.0 34 30736.0 35 42925.0 36 62847.0 37 108371.0 38 256511.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.093657241784435 19.884719506926466 10.920410148661794 28.101213102627305 2 13.618698205358907 20.88443841517139 41.02469671047213 24.472166668997573 3 18.206066101126524 25.070330257225542 29.43856892675717 27.285034714890767 4 11.807019450179288 17.70137067105447 38.60619984166654 31.885410037099703 5 12.674407774224598 38.877642894843056 35.47874817597566 12.96920115495669 6 31.229546881157354 38.4611310915443 17.88628650020171 12.423035527096635 7 27.714189896254805 32.02019010119893 21.820804312521155 18.44481569002512 8 24.451561522447516 38.92077429530837 19.800759764463702 16.826904417780415 9 25.865133688013174 16.05420485012008 20.277761822187102 37.80289963967964 10 14.52082864394545 30.138391921575327 34.28316399791985 21.057615436559374 11 33.708487858188484 23.173217382125156 24.586799631779364 18.531495127906993 12 22.958303775028835 25.374297391987362 31.862895001140938 19.804503831842872 13 28.783139580911104 22.57178205142136 25.712697937153738 22.932380430513792 14 20.224026674573004 23.1620483414544 27.646152102811357 28.967772881161235 15 22.32833374211796 31.94977848012841 23.351951144545207 22.36993663320842 16 21.37430767702357 29.023855649107123 28.397349924248065 21.20448674962124 17 20.863072600549508 29.52422346750283 28.17497399916176 21.437729932785892 18 20.677638278320046 28.467203139358716 30.239767280133123 20.61539130218811 19 22.93824354866751 27.537224002185628 27.663425477485532 21.861106971661325 20 22.884760464820257 28.258041682319007 28.500200246505 20.35699760635573 21 22.188015239443704 27.394514669672326 27.446989526763733 22.970480564120237 22 22.414585306033747 28.98744198320424 27.482808400987256 21.115164309774762 23 21.528572249068237 28.610124974969303 28.04369389437279 21.81760888158967 24 22.530541283121963 27.468527343568088 28.891355303394857 21.10957606991509 25 21.904795909179818 28.0688137056137 28.511706464402476 21.514683920804007 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 219.0 1 219.0 2 233.0 3 247.0 4 247.0 5 247.0 6 410.5 7 574.0 8 574.0 9 574.0 10 961.5 11 1349.0 12 1349.0 13 1349.0 14 2212.5 15 3076.0 16 3076.0 17 3076.0 18 5506.0 19 7936.0 20 7936.0 21 7936.0 22 12474.5 23 17013.0 24 17013.0 25 17013.0 26 24308.0 27 31603.0 28 31603.0 29 31603.0 30 41662.0 31 51721.0 32 51721.0 33 51721.0 34 58760.5 35 65800.0 36 65800.0 37 65800.0 38 70957.0 39 76114.0 40 76114.0 41 76114.0 42 79159.0 43 82204.0 44 82204.0 45 82204.0 46 84280.0 47 86356.0 48 86356.0 49 86356.0 50 82236.5 51 78117.0 52 78117.0 53 78117.0 54 67715.0 55 57313.0 56 57313.0 57 57313.0 58 48458.5 59 39604.0 60 39604.0 61 39604.0 62 31703.5 63 23803.0 64 23803.0 65 23803.0 66 18155.0 67 12507.0 68 12507.0 69 12507.0 70 9028.0 71 5549.0 72 5549.0 73 5549.0 74 3788.5 75 2028.0 76 2028.0 77 2028.0 78 1398.0 79 768.0 80 768.0 81 768.0 82 495.0 83 222.0 84 222.0 85 222.0 86 146.5 87 71.0 88 71.0 89 71.0 90 41.5 91 12.0 92 12.0 93 12.0 94 7.5 95 3.0 96 3.0 97 3.0 98 2.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.11424845935331647 2 0.10602132844879776 3 0.016454261809037425 4 0.0 5 0.004656866549727573 6 0.3404169447850856 7 0.020490212818801323 8 0.009779419754427903 9 0.009468961984446066 10 0.0038807221247729776 11 0.004812095434718493 12 4.6568665497275733E-4 13 4.6568665497275733E-4 14 1.552288849909191E-4 15 0.0037254932397820587 16 3.104577699818382E-4 17 0.0 18 3.104577699818382E-4 19 3.104577699818382E-4 20 6.209155399636764E-4 21 0.014281057419164559 22 0.0 23 1.552288849909191E-4 24 0.0 25 0.005588239859673088 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 644210.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 83.91216536423259 #Duplication Level Percentage of deduplicated Percentage of total 1 92.3604580808471 77.50166031596314 2 5.870074999201135 9.851414080668263 3 0.8859213796698292 2.2301874393169134 4 0.3083748685366869 1.035056118512958 5 0.1558335200494208 0.6538164051838726 6 0.09328911672663524 0.4696855073669169 7 0.06304813535813308 0.3703353892054728 8 0.03873641139275609 0.26003649267247136 9 0.02711705453623807 0.20479056879921498 >10 0.16747980569776416 2.6421664977189607 >50 0.01409175866661371 0.8162129507966094 >100 0.013720718208874895 2.3526134030633346 >500 9.270755543860556E-4 0.6445921121245295 >1k 9.270755543860556E-4 0.9674327186073677 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 1489 0.23113580975147857 No Hit GTCCTACAGTGGACATTTCTAAATT 1402 0.2176308967572686 No Hit CTGTAGGACGTGGAATATGGCAAGA 1258 0.19527793731857623 No Hit GTCCTACAGTGTGCATTTCTCATTT 1042 0.1617484981605377 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1027 0.15942006488567392 No Hit GGTATCAACGCAGAGTACTTTTTTT 986 0.15305568060104624 No Hit CTTTAGGACGTGAAATATGGCGAGG 936 0.1452942363515003 No Hit CTGTAGGACCTGGAATATGGCGAGA 897 0.13924030983685443 No Hit TATCAACGCAGAGTACTTTTTTTTT 755 0.11719780816814393 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 1.552288849909191E-4 12 0.0 0.0 0.0 0.0 3.104577699818382E-4 13 0.0 0.0 0.0 0.0 3.104577699818382E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGCACCG 25 0.0059347497 19.05152 5 TGCGCTG 25 0.0060750553 18.974615 10 CCAACGA 55 5.9795275E-7 17.249651 19 GGCTTAG 50 0.0014598743 13.345425 1 TCCAACG 90 5.476577E-7 12.649745 18 TAGGACC 325 0.0 12.310213 4 CCTAATG 55 0.0029912747 12.130313 2 TAGTACT 55 0.0030035311 12.123695 4 CGTTGAT 115 7.183553E-8 11.548869 14 GTCTAGG 75 2.0056576E-4 11.438935 1 GTATAGC 100 1.846267E-6 11.438935 1 TCTACAC 175 1.8189894E-12 11.432694 3 GGCGAGG 225 0.0 11.3847685 19 TGTAGAA 235 0.0 11.356037 2 ATTAACC 85 5.158629E-5 11.208524 3 TACACTC 85 5.1668314E-5 11.206777 5 GTGTTAG 60 0.005722186 11.121188 1 GTGCTAG 60 0.005722186 11.121188 1 TCATGAC 60 0.0057454915 11.115119 3 GGTATCA 455 0.0 11.103729 1 >>END_MODULE