FastQCFastQC Report
Thu 26 May 2016
SRR1926910_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926910_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences628170
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT16710.2660107932566025No Hit
CTGTAGGACGTGGAATATGGCAAGA13390.21315885827085024No Hit
GTCCTAAAGTGTGTATTTCTCATTT12620.20090102997596193No Hit
GTCCTACAGTGTGCATTTCTCATTT11310.18004680261712594No Hit
CTTTAGGACGTGAAATATGGCGAGG10220.16269481191397234No Hit
GTATCAACGCAGAGTACTTTTTTTT8890.14152219940461977No Hit
GTACATGGGAAGCAGTGGTATCAAC8690.13833834789945398No Hit
CCCATGTACTCTGCGTTGATACCAC7410.11796169826639286No Hit
CTGTAGGACCTGGAATATGGCGAGA7190.11445946161071048No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC2800.013.2592454
TCAGAAC655.2909953E-513.19343953
GTATTAA957.016388E-813.047481
CCCTTAT603.9621876E-412.7129291
GCCATTA550.002982356812.1350691
TATAGGA1103.641435E-812.1273032
TAGGCTG550.003025548512.1118035
GTATAGA1209.438736E-911.9183711
AAATGTC1655.456968E-1211.5332077
AGGACCT4750.011.4196995
TGTAGGA9300.011.2703812
GGACCTG4650.011.2541786
TAGAACC1104.820249E-711.2502694
GTCTAGA855.1172057E-511.2172911
GATATAC1701.0913936E-1111.2172911
GTAGAAC2550.011.2101133
TTGTAGA1201.2072087E-711.1238131
CTTAGAC600.005739154811.1166953
GTTTTAT951.3027555E-511.0401751
GTAGGAC8650.011.01571753
Produced by FastQC (version 0.11.5)