##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926910_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 628170 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.81750322364965 31.0 28.0 31.0 16.0 34.0 2 28.021339764713375 31.0 28.0 31.0 16.0 34.0 3 27.480145502013787 31.0 27.0 31.0 10.0 34.0 4 31.15345527484598 35.0 32.0 37.0 17.0 37.0 5 31.061096518458378 35.0 32.0 37.0 16.0 37.0 6 30.828661031249503 35.0 32.0 37.0 11.0 37.0 7 30.92484996099782 35.0 32.0 37.0 11.0 37.0 8 30.533255328971457 35.0 31.0 37.0 10.0 37.0 9 32.71564863014789 37.0 32.0 39.0 17.0 39.0 10 32.889537863954025 37.0 33.0 39.0 16.0 39.0 11 33.03326965630323 37.0 33.0 39.0 17.0 39.0 12 32.915498989127144 37.0 33.0 39.0 11.0 39.0 13 33.02834105417323 38.0 33.0 39.0 15.0 39.0 14 34.012068389130334 39.0 34.0 40.0 13.0 41.0 15 33.5845933425665 39.0 33.0 40.0 10.0 41.0 16 33.66104716876005 39.0 33.0 40.0 10.0 41.0 17 33.87625324354872 39.0 33.0 40.0 10.0 41.0 18 33.92653103459255 39.0 34.0 41.0 10.0 41.0 19 33.29604088065333 39.0 33.0 40.0 8.0 41.0 20 33.58968909690052 39.0 33.0 41.0 2.0 41.0 21 33.56567012114555 39.0 33.0 41.0 2.0 41.0 22 33.5920499227916 39.0 34.0 41.0 2.0 41.0 23 33.58065332632886 39.0 34.0 41.0 2.0 41.0 24 32.28651957272713 38.0 32.0 40.0 2.0 41.0 25 32.86163458936275 39.0 33.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.6918099658268879 1101 2 -0.7488596047589553 1101 3 -1.8678923090739694 1101 4 -1.3554389452544129 1101 5 -1.3807112487304565 1101 6 -3.0704358634165807 1101 7 -1.4358283766003375 1101 8 -1.1330364993319648 1101 9 -0.8731931040738985 1101 10 -9.799116032321432 1101 11 -4.827631935171549 1101 12 -2.5153599016864234 1101 13 -1.4804452592393282 1101 14 -1.290380259186044 1101 15 -3.0314323342966283 1101 16 -1.084569997106442 1101 17 -0.9499223155118344 1101 18 -0.8360789483628537 1101 19 -1.427143050396495 1101 20 -0.9080118178700829 1101 21 -1.0841068363751134 1101 22 -0.8078233218409494 1101 23 -0.8401175114992938 1101 24 -0.9174416239931347 1101 25 -0.9601415394409258 1102 1 -0.6583889388367297 1102 2 -0.6772744375146225 1102 3 -1.0761543043134587 1102 4 -0.8989969413847554 1102 5 -0.5702652638471726 1102 6 -1.2874533047105494 1102 7 -0.3130642168586846 1102 8 0.06546435672516182 1102 9 0.025614679782115957 1102 10 0.15933039798994741 1102 11 -0.37062966627217264 1102 12 -0.07009187924798255 1102 13 -0.37955579500581393 1102 14 -0.6284184268061992 1102 15 0.09834383808404823 1102 16 -0.20795010039008588 1102 17 -0.24795547206588964 1102 18 -0.6233313529786031 1102 19 -0.07908971038173718 1102 20 -0.1435597640289643 1102 21 -0.43569576191902115 1102 22 -0.35322218878322076 1102 23 -0.7162989399315265 1102 24 -4.564575572574029 1102 25 -2.252251894252243 1103 1 -0.18708496110188122 1103 2 -0.25128735718670825 1103 3 -0.24566093684259727 1103 4 -0.39197223178769747 1103 5 -0.4479840660032721 1103 6 0.26496072198000675 1103 7 0.19843825766629664 1103 8 0.344053977758513 1103 9 0.13689365601285886 1103 10 0.004159520954118534 1103 11 -0.1320257395653499 1103 12 -0.47383386016038287 1103 13 -0.1359159147099831 1103 14 -0.4213348901406704 1103 15 -0.03742859029288326 1103 16 0.03955587701943131 1103 17 -0.22128608687560813 1103 18 -0.3102354225193267 1103 19 -2.254252877506321 1103 20 -0.8356067454650145 1103 21 -0.653780406048682 1103 22 -0.6840731980908217 1103 23 -0.5889205103022945 1103 24 0.939397482845969 1103 25 0.2520921727927856 1104 1 -0.4459314919691373 1104 2 -0.4535979579429039 1104 3 -1.2898435012324185 1104 4 -0.7476927191144043 1104 5 -0.4114550299923714 1104 6 -1.8067131439943793 1104 7 -0.23927139299672007 1104 8 0.13400474957456865 1104 9 0.2555454285506471 1104 10 0.33300271249161284 1104 11 0.04245531781725731 1104 12 -0.16534980815301026 1104 13 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21 -0.6325644639402483 1108 22 -0.2695252439891149 1108 23 -0.37015930878246905 1108 24 -1.2989054948079186 1108 25 -0.6284326803487019 1109 1 0.15201989908731406 1109 2 0.3679191987668382 1109 3 -0.810128787455028 1109 4 0.37335936486919863 1109 5 0.5692574572623457 1109 6 0.5301482913844424 1109 7 0.6719142823471422 1109 8 0.9740020444535666 1109 9 0.566266031168368 1109 10 0.6113700679237013 1109 11 0.24873848156031642 1109 12 0.07046391305305377 1109 13 0.06879064541826097 1109 14 0.23058672563636406 1109 15 -0.8339932272189685 1109 16 0.2267662570008966 1109 17 -0.022733038924968696 1109 18 0.20704158572154086 1109 19 -1.4814903095630285 1109 20 -0.15211811318119572 1109 21 0.12446772159703556 1109 22 0.25256107288079477 1109 23 0.3267317035426913 1109 24 -0.6184534635591916 1109 25 0.0897463236999485 1110 1 0.17126867332537188 1110 2 0.16600109691156462 1110 3 0.5688657181748553 1110 4 0.33286610348008594 1110 5 0.3042341739450265 1110 6 0.45935591944268594 1110 7 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1 0.5916699950724293 2215 2 -0.03167625047882794 2215 3 0.6019093945986995 2215 4 0.7828448139970874 2215 5 0.9551706113449505 2215 6 1.2646081248532148 2215 7 0.6471907548058908 2215 8 -0.5951926227784092 2215 9 -0.04575978470509767 2215 10 -0.10260018257977777 2215 11 -0.3699444613550398 2215 12 -0.1548655573211093 2215 13 0.003844644405411657 2215 14 -0.09124606005184432 2215 15 0.007563615989646166 2215 16 0.21235082400792749 2215 17 -0.20770754103899236 2215 18 -0.8229522900749018 2215 19 0.4693099589597409 2215 20 -3.840982073420985 2215 21 -2.011142166636251 2215 22 -0.8219274972354427 2215 23 -0.9752592514797414 2215 24 -0.6892262909390929 2215 25 -0.5671098399576096 2216 1 -0.7777190567359789 2216 2 -1.1545306004299505 2216 3 -0.3107191489008123 2216 4 -0.47072898854445455 2216 5 -0.6101441491633572 2216 6 -1.7501328331135504 2216 7 -9.559499372271333 2216 8 -10.831114421409886 2216 9 -6.029786177666971 2216 10 -3.6846138678192695 2216 11 -2.7632856148252287 2216 12 -2.3074637977903194 2216 13 -2.029570800364869 2216 14 -2.254336969142752 2216 15 -3.3779691308334243 2216 16 -2.490751815288263 2216 17 -2.39002621161573 2216 18 -2.277356004639909 2216 19 -1.7035023577558022 2216 20 -2.730704458562723 2216 21 -5.208158784427059 2216 22 -4.090728083745709 2216 23 -3.857814481196261 2216 24 -4.091883182434692 2216 25 -3.5468038771032617 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 41533.0 3 13.0 4 306.0 5 1817.0 6 2116.0 7 1829.0 8 1520.0 9 1403.0 10 1349.0 11 1263.0 12 1239.0 13 1269.0 14 1248.0 15 1368.0 16 1304.0 17 1498.0 18 1641.0 19 2120.0 20 2680.0 21 3476.0 22 4244.0 23 5060.0 24 5809.0 25 6736.0 26 7758.0 27 8417.0 28 9483.0 29 11215.0 30 13395.0 31 15931.0 32 20654.0 33 27243.0 34 36598.0 35 51017.0 36 73390.0 37 114945.0 38 145283.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.49497431315613 19.074156801429528 12.944414308050673 25.48645457736367 2 13.640428553893633 20.054549599167252 43.426550128403576 22.878471718535547 3 18.852422429954217 25.427862726843763 29.465458533661508 26.25425630954051 4 11.896815674229885 17.128515239808134 39.10847162239877 31.86619746356321 5 12.29791292799944 38.382411717029534 36.201392018267256 13.118283336703772 6 31.41244377214283 37.67397794673779 18.527128697106008 12.386449584013375 7 27.113107781907352 31.398552945834453 23.323850211374637 18.16448906088356 8 23.961170194085174 38.087020653676575 21.108319258779172 16.843489893459086 9 26.001085912907236 15.736183668952602 20.58617005523507 37.676560362905086 10 14.587437511645648 29.748836231540153 34.92643135955867 20.737294897255527 11 33.62465230398861 22.804718634914014 25.064523781849914 18.506105279247464 12 23.18598978435044 25.01289693912645 32.4467881844931 19.35432509203001 13 28.901261969283482 22.61784589517662 25.677556180401425 22.803335955138472 14 20.032544764973935 23.31440726811855 27.808418967674957 28.84462899923255 15 22.645477878191535 32.312743206149655 23.16532604749804 21.876452868160772 16 20.939289768791923 28.686615216328665 29.471267369265775 20.902827645613637 17 21.000968072707355 29.44103354499309 28.52805181736544 21.029946564934114 18 20.80484659353257 28.20879838234592 30.73001417039502 20.256340853726496 19 23.615715573219706 27.136656189193236 27.3328301598372 21.914798077749857 20 23.004244830891416 28.29866031481169 28.560260358119795 20.136834496177105 21 22.245167330341094 27.504302871181352 27.32199806072263 22.928531737754927 22 22.275242971964285 29.273822716445668 27.604385596188873 20.846548715401177 23 21.527198384847114 28.676924840618295 28.187315699973887 21.608561074560704 24 23.078711222351718 27.616595655247522 28.584006827286217 20.72068629511454 25 22.02291486217965 28.075482753349977 28.49980256789117 21.401799816579203 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 265.0 1 265.0 2 303.5 3 342.0 4 342.0 5 342.0 6 543.0 7 744.0 8 744.0 9 744.0 10 1054.5 11 1365.0 12 1365.0 13 1365.0 14 2243.0 15 3121.0 16 3121.0 17 3121.0 18 5483.5 19 7846.0 20 7846.0 21 7846.0 22 12417.5 23 16989.0 24 16989.0 25 16989.0 26 24633.0 27 32277.0 28 32277.0 29 32277.0 30 42769.0 31 53261.0 32 53261.0 33 53261.0 34 59721.0 35 66181.0 36 66181.0 37 66181.0 38 70277.5 39 74374.0 40 74374.0 41 74374.0 42 77320.0 43 80266.0 44 80266.0 45 80266.0 46 81985.5 47 83705.0 48 83705.0 49 83705.0 50 78484.5 51 73264.0 52 73264.0 53 73264.0 54 63358.0 55 53452.0 56 53452.0 57 53452.0 58 45114.0 59 36776.0 60 36776.0 61 36776.0 62 29732.0 63 22688.0 64 22688.0 65 22688.0 66 17491.0 67 12294.0 68 12294.0 69 12294.0 70 8896.5 71 5499.0 72 5499.0 73 5499.0 74 3863.5 75 2228.0 76 2228.0 77 2228.0 78 1539.0 79 850.0 80 850.0 81 850.0 82 558.5 83 267.0 84 267.0 85 267.0 86 180.0 87 93.0 88 93.0 89 93.0 90 55.5 91 18.0 92 18.0 93 18.0 94 11.0 95 4.0 96 4.0 97 4.0 98 2.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.22127767960902303 2 0.13547288154480475 3 0.13786077017367912 4 0.06988554053838929 5 0.02563000461658468 6 0.023082923412452043 7 0.28559148001337215 8 0.2776318512504577 9 0.020217457057802826 10 0.04027572154034736 11 0.017033605552637023 12 0.018147953579445056 13 0.016556027826862156 14 0.017829568428928473 15 0.016237642676345577 16 0.019262301606253085 17 0.018784723880478214 18 0.015919257525828994 19 0.02483404174029323 20 0.017829568428928473 21 0.016078450101087285 22 0.018147953579445056 23 0.018784723880478214 24 0.016237642676345577 25 0.016874412977378735 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 628170.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 83.96265463150337 #Duplication Level Percentage of deduplicated Percentage of total 1 93.39706907849774 78.41865854632569 2 4.930872215181542 8.280182416707275 3 0.8084439804828987 2.0363730816661043 4 0.28140987078801843 0.9451167916348152 5 0.15458696640245664 0.6489766035290642 6 0.08399995214582218 0.42317153826494874 7 0.05883333105951322 0.34578618595995664 8 0.04676308583961648 0.31410822606840516 9 0.03152538329910826 0.23822593830619124 >10 0.16929724470425178 2.6442627592892065 >50 0.019738258392226916 1.1898906290560838 >100 0.015372948801943394 2.8065126223553842 >500 0.0011387369480688094 0.6848961645320183 >1k 9.489474567240079E-4 1.0238384963048435 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 1671 0.2660107932566025 No Hit CTGTAGGACGTGGAATATGGCAAGA 1339 0.21315885827085024 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1262 0.20090102997596193 No Hit GTCCTACAGTGTGCATTTCTCATTT 1131 0.18004680261712594 No Hit CTTTAGGACGTGAAATATGGCGAGG 1022 0.16269481191397234 No Hit GTATCAACGCAGAGTACTTTTTTTT 889 0.14152219940461977 No Hit GTACATGGGAAGCAGTGGTATCAAC 869 0.13833834789945398 No Hit CCCATGTACTCTGCGTTGATACCAC 741 0.11796169826639286 No Hit CTGTAGGACCTGGAATATGGCGAGA 719 0.11445946161071048 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 3.183851505165799E-4 0.0 8 0.0 0.0 0.0 3.183851505165799E-4 0.0 9 0.0 0.0 0.0 3.183851505165799E-4 0.0 10 0.0 0.0 0.0 3.183851505165799E-4 0.0 11 0.0 0.0 0.0 3.183851505165799E-4 0.0 12 0.0 0.0 0.0 3.183851505165799E-4 0.0 13 0.0 0.0 0.0 3.183851505165799E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGACC 280 0.0 13.259245 4 TCAGAAC 65 5.2909953E-5 13.1934395 3 GTATTAA 95 7.016388E-8 13.04748 1 CCCTTAT 60 3.9621876E-4 12.712929 1 GCCATTA 55 0.0029823568 12.135069 1 TATAGGA 110 3.641435E-8 12.127303 2 TAGGCTG 55 0.0030255485 12.111803 5 GTATAGA 120 9.438736E-9 11.918371 1 AAATGTC 165 5.456968E-12 11.533207 7 AGGACCT 475 0.0 11.419699 5 TGTAGGA 930 0.0 11.270381 2 GGACCTG 465 0.0 11.254178 6 TAGAACC 110 4.820249E-7 11.250269 4 GTCTAGA 85 5.1172057E-5 11.217291 1 GATATAC 170 1.0913936E-11 11.217291 1 GTAGAAC 255 0.0 11.210113 3 TTGTAGA 120 1.2072087E-7 11.123813 1 CTTAGAC 60 0.0057391548 11.116695 3 GTTTTAT 95 1.3027555E-5 11.040175 1 GTAGGAC 865 0.0 11.0157175 3 >>END_MODULE