FastQCFastQC Report
Thu 26 May 2016
SRR1926910_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926910_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences628170
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT16880.2687170670359934No Hit
CTGTAGGACGTGGAATATGGCAAGA16460.26203097887514526No Hit
GTATCAACGCAGAGTACTTTTTTTT15290.24340544756992535No Hit
GTCCTAAAGTGTGTATTTCTCATTT12900.20535842208319402No Hit
CTTTAGGACGTGAAATATGGCGAGG12410.19755798589553786No Hit
GTCCTACAGTGTGCATTTCTCATTT11800.18784723880478216No Hit
GTACATGGGAAGCAGTGGTATCAAC10420.16587866341913812No Hit
GGTATCAACGCAGAGTACTTTTTTT10190.16221723418819747No Hit
CCCATGTACTCTGCGTTGATACCAC9360.14900425044175938No Hit
CTGTAGGACCTGGAATATGGCGAGA8990.14311412515720265No Hit
TATCAACGCAGAGTACTTTTTTTTT8150.12974194883550633No Hit
CTGAAGGACCTGGAATATGGCGAGA7080.11270834328286929No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGCTTA400.005208608714.2770066
GACAATC400.005311562714.2349557
TCCAACG1101.8735591E-1013.80579118
GTACTAA707.0240185E-613.6123631
GTGTAAG707.0240185E-613.6123631
GATATAC2250.013.5518661
TAGGACC3400.013.157244
CCAACGA802.0141833E-613.05078619
GGCGAGG3600.012.65530819
TATTTAG550.003000151112.125442
AGGACGT8900.011.7638245
TCTTAGG657.82226E-411.72572
CTGTAGG10250.011.7132731
TTAGGAC4800.011.7001493
GACGTGA4300.011.6969397
GGACGTG8800.011.6811876
ACTGTTC2550.011.5359078
TGTAGGA10450.011.4872592
TGGCGAG9100.011.16031318
GGACCTG5900.011.1312266
Produced by FastQC (version 0.11.5)