##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926910_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 628170 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.757845806071604 31.0 30.0 33.0 25.0 34.0 2 30.085242848273555 31.0 30.0 34.0 26.0 34.0 3 30.13532960822707 31.0 30.0 34.0 26.0 34.0 4 33.69357180381107 35.0 35.0 37.0 28.0 37.0 5 33.68531289300667 37.0 35.0 37.0 28.0 37.0 6 33.59986309438528 37.0 35.0 37.0 28.0 37.0 7 33.59539774264928 37.0 35.0 37.0 28.0 37.0 8 33.620282726013656 37.0 35.0 37.0 28.0 37.0 9 35.14907747902638 39.0 35.0 39.0 29.0 39.0 10 35.041993409427384 39.0 35.0 39.0 28.0 39.0 11 35.04990687234347 39.0 35.0 39.0 29.0 39.0 12 34.961039209131286 39.0 35.0 39.0 28.0 39.0 13 34.96189566518618 39.0 35.0 39.0 28.0 39.0 14 36.048474139166146 40.0 36.0 41.0 27.0 41.0 15 36.026007291019944 40.0 36.0 41.0 27.0 41.0 16 36.016605377525195 40.0 36.0 41.0 27.0 41.0 17 35.9964245347597 40.0 36.0 41.0 27.0 41.0 18 35.96679720457838 40.0 36.0 41.0 27.0 41.0 19 36.03340019421494 40.0 36.0 41.0 27.0 41.0 20 35.9976264387029 40.0 36.0 41.0 27.0 41.0 21 35.944316029100406 40.0 36.0 41.0 27.0 41.0 22 35.84550838148909 40.0 36.0 41.0 27.0 41.0 23 35.783123995096865 40.0 36.0 41.0 27.0 41.0 24 35.70951494022319 40.0 36.0 41.0 27.0 41.0 25 35.57176082907493 40.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.08267864280719905 1101 2 0.17729551711278546 1101 3 0.3229314280849813 1101 4 0.43203633879608105 1101 5 0.24383919945558574 1101 6 0.1539362227295058 1101 7 0.09500216509325554 1101 8 0.01981226240828704 1101 9 -0.05326767030486934 1101 10 0.009057972385953406 1101 11 -0.026387347500538283 1101 12 0.08432027772502693 1101 13 -0.11427881836469567 1101 14 -0.04329344381173428 1101 15 0.19051473771488503 1101 16 0.1376562307980791 1101 17 0.15608072013505847 1101 18 0.07770246055440566 1101 19 -0.06035870055705317 1101 20 -0.03183270405235561 1101 21 -0.08008700071527386 1101 22 0.11490783258051351 1101 23 0.10989388723887572 1101 24 0.14479082442888824 1101 25 0.1290167095369199 1102 1 -0.4802554755931574 1102 2 -0.38982492942888314 1102 3 -0.22139562749609354 1102 4 -0.11973075798709232 1102 5 -0.18811817864327196 1102 6 -0.22261407584651494 1102 7 -0.17921450783724424 1102 8 -0.412729740069409 1102 9 -0.22953540043111786 1102 10 -0.24192336139280712 1102 11 -0.317585966673434 1102 12 -0.3155384380500905 1102 13 0.13839375022427447 1102 14 -0.1480282052989068 1102 15 0.013025560225706556 1102 16 -0.14634594066389894 1102 17 -0.14955603325088873 1102 18 -0.04671117511325207 1102 19 -0.34728051400620075 1102 20 -0.41309479672924 1102 21 -0.2832996508732606 1102 22 -0.5028278615217445 1102 23 -0.12346519740638229 1102 24 -0.2671744878579716 1102 25 -0.3629029463698714 1103 1 -0.8772735671450107 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25 -0.04471837599201933 2213 1 0.43473059085914656 2213 2 0.4873647811820483 2213 3 0.6193599995135557 2213 4 0.660291750051492 2213 5 0.5961465587629462 2213 6 0.5325552703485599 2213 7 0.40065151574260227 2213 8 0.3338468944429209 2213 9 0.3978665288293328 2213 10 0.4506597039876894 2213 11 0.5712446871314967 2213 12 0.5177445201492716 2213 13 0.5864441253582484 2213 14 0.27809183757354816 2213 15 0.5260255602257047 2213 16 0.461431122572975 2213 17 0.4482928413471825 2213 18 0.4170444518963947 2213 19 0.34919108299272494 2213 20 0.5005525773329254 2213 21 0.25275715512888297 2213 22 0.29841865509133214 2213 23 0.3136687790137671 2213 24 0.3346090062470708 2213 25 0.15967904719925485 2214 1 0.5097970008687724 2214 2 0.5255803730973518 2214 3 0.5189990755482015 2214 4 0.6195188915336871 2214 5 0.5745132575117395 2214 6 0.6652222578365254 2214 7 0.5053242780140152 2214 8 0.6857737471859409 2214 9 0.373248627000649 2214 10 0.21106393882887886 2214 11 0.11892803650589201 2214 12 0.14604192149671746 2214 13 0.30851053536787276 2214 14 0.5992564189017457 2214 15 0.17856935233350413 2214 16 0.4278911803208061 2214 17 0.47483855838279254 2214 18 0.24613781089543352 2214 19 0.33507751225162963 2214 20 0.493296561933505 2214 21 0.17777832933484916 2214 22 0.3154263548025966 2214 23 0.5548449099088586 2214 24 0.1889766674597695 2214 25 0.2915106160154295 2215 1 0.09206978929512033 2215 2 -0.029309705621468396 2215 3 -0.07729200439651507 2215 4 0.10868764447964452 2215 5 0.017858191216511443 2215 6 0.15732262127719565 2215 7 -0.10566813235124073 2215 8 -0.058563662741832445 2215 9 -0.4335567360778043 2215 10 -0.020092984184351792 2215 11 -0.18334866575218456 2215 12 -0.1647022051684246 2215 13 -0.015375033977036878 2215 14 0.023282453409329662 2215 15 -0.02118753850351851 2215 16 -0.23254346198226017 2215 17 -0.02353804819338734 2215 18 -0.17044723920820104 2215 19 -0.36796238719300334 2215 20 -0.34551096127899683 2215 21 -0.18328487810669714 2215 22 -0.22118349544629723 2215 23 -0.14911323467146786 2215 24 -0.38991103285361817 2215 25 -0.40722124605587595 2216 1 -0.6872694091408533 2216 2 -0.4136352188179515 2216 3 -2.3656400004864473 2216 4 -1.9077082499485094 2216 5 -0.8468534412370587 2216 6 -0.4784447296514429 2216 7 -0.48334848425739807 2216 8 -0.4751531055570837 2216 9 -0.7941334711706673 2216 10 -0.6483402960123144 2216 11 -0.7277553128685028 2216 12 -0.615255479850731 2216 13 -0.68555587464175 2216 14 -0.876908162426453 2216 15 -0.8449744397742904 2216 16 -0.7025688774270265 2216 17 -1.0657071586528204 2216 18 -0.9069555481036033 2216 19 -1.0788089170072723 2216 20 -1.028447422667071 2216 21 -1.0262428448711134 2216 22 -1.089581344908666 2216 23 -1.2453312209862304 2216 24 -1.3643909937529344 2216 25 -1.4383209528007441 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 16469.0 3 27.0 4 284.0 5 1751.0 6 2424.0 7 1775.0 8 1409.0 9 1150.0 10 998.0 11 959.0 12 906.0 13 835.0 14 804.0 15 857.0 16 868.0 17 934.0 18 1024.0 19 1155.0 20 1555.0 21 2004.0 22 2539.0 23 3128.0 24 3675.0 25 4231.0 26 5137.0 27 5752.0 28 6509.0 29 8048.0 30 10255.0 31 13175.0 32 17027.0 33 22538.0 34 29913.0 35 41909.0 36 61648.0 37 106885.0 38 247613.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.157102240128516 19.92433032014586 10.751055040607907 28.167512399117715 2 13.600216736521698 21.060893400691644 40.96814291861225 24.37074694417441 3 18.4255358445024 25.237472017628498 29.402427102538876 26.934565035330234 4 11.855071079484853 17.956922489135106 38.18042886479775 32.0075775665823 5 12.469712069640748 39.045878453460865 35.63150135798184 12.852908118916545 6 31.123941407367056 38.45473033745561 17.900499880980718 12.520828374196618 7 27.595453090691386 32.022623087076916 21.920255528787592 18.461668293444113 8 24.35787010133656 39.08677689239856 19.950963612771748 16.604389393493125 9 26.024871716899934 15.83068382096567 20.07664348044827 38.067800981686126 10 14.74850552022224 30.25766343758209 34.02653845847695 20.967292583718727 11 33.55706514384693 23.006910956755974 24.817514762001043 18.61850913739606 12 23.357164575662203 25.26732540868909 31.902185246916826 19.47332476873188 13 29.02936175903108 22.63533090084755 25.601753682159433 22.733553657961934 14 20.066383303882706 23.632774567394176 27.49032905105306 28.81051307767006 15 22.299875666046322 32.540472214306526 23.104623599253042 22.055028520394114 16 20.98808276793077 28.879786553235924 29.151211622405544 20.98091905642776 17 20.71146458994315 29.766034299686865 28.249722606496025 21.27277850387396 18 20.580004075342902 28.231937952904318 30.8318793698501 20.356178601902673 19 23.072967741113843 27.317055310044445 27.6384661428153 21.971510806026412 20 22.9575893034814 28.34071194443516 28.519326866900048 20.182371885183397 21 22.00452171697873 27.363074767545537 27.365622213730735 23.266781301745002 22 22.411449130012574 29.344444975086365 27.206488689367532 21.037617205533536 23 21.45823814928785 28.742901989751168 28.159778658291003 21.63908120266998 24 22.698827863202418 27.605469228822184 28.989969259864782 20.70573364811062 25 21.97788026943596 28.06582108395277 28.468446667632502 21.48785197897877 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 293.0 1 293.0 2 279.0 3 265.0 4 265.0 5 265.0 6 438.0 7 611.0 8 611.0 9 611.0 10 988.5 11 1366.0 12 1366.0 13 1366.0 14 2116.5 15 2867.0 16 2867.0 17 2867.0 18 5074.5 19 7282.0 20 7282.0 21 7282.0 22 11820.0 23 16358.0 24 16358.0 25 16358.0 26 23940.5 27 31523.0 28 31523.0 29 31523.0 30 42048.0 31 52573.0 32 52573.0 33 52573.0 34 58991.0 35 65409.0 36 65409.0 37 65409.0 38 69345.0 39 73281.0 40 73281.0 41 73281.0 42 76624.0 43 79967.0 44 79967.0 45 79967.0 46 82429.5 47 84892.0 48 84892.0 49 84892.0 50 80139.5 51 75387.0 52 75387.0 53 75387.0 54 64894.0 55 54401.0 56 54401.0 57 54401.0 58 45904.0 59 37407.0 60 37407.0 61 37407.0 62 30263.0 63 23119.0 64 23119.0 65 23119.0 66 17700.0 67 12281.0 68 12281.0 69 12281.0 70 8911.0 71 5541.0 72 5541.0 73 5541.0 74 3861.0 75 2181.0 76 2181.0 77 2181.0 78 1492.0 79 803.0 80 803.0 81 803.0 82 534.0 83 265.0 84 265.0 85 265.0 86 172.5 87 80.0 88 80.0 89 80.0 90 47.0 91 14.0 92 14.0 93 14.0 94 9.0 95 4.0 96 4.0 97 4.0 98 2.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.11238995813235271 2 0.10825095117563716 3 0.015282487224795836 4 0.0 5 0.0050941624082652785 6 0.353566709648662 7 0.022127767960902302 8 0.010347517391788847 9 0.010665902542305428 10 0.0039798143814572485 11 0.005890125284556728 12 4.7757772577486986E-4 13 9.551554515497397E-4 14 0.0 15 0.0036614292309406684 16 6.367703010331598E-4 17 1.5919257525828995E-4 18 3.183851505165799E-4 19 3.183851505165799E-4 20 4.7757772577486986E-4 21 0.014327331773246098 22 0.0 23 1.5919257525828995E-4 24 1.5919257525828995E-4 25 0.006845280736106468 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 628170.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 80.85542101117275 #Duplication Level Percentage of deduplicated Percentage of total 1 91.6583969959759 74.11078278318843 2 6.2345924958956385 10.082012021774803 3 1.0537505746449196 2.5560433906104056 4 0.3526244673946826 1.1404639908015048 5 0.17861346777124332 0.7220933567454708 6 0.12401814771339985 0.6016523727835654 7 0.07471902479993923 0.4229006745420341 8 0.043691206315149514 0.28261367048779384 9 0.03653843552871932 0.2658897528987773 >10 0.2010015091977755 2.9912443395940325 >50 0.02132306286205744 1.2274509517834473 >100 0.017966530314012852 3.188737381061704 >500 0.0011846063942497704 0.7103820448186442 >1k 0.0015794751923330272 1.6977332689092766 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 1688 0.2687170670359934 No Hit CTGTAGGACGTGGAATATGGCAAGA 1646 0.26203097887514526 No Hit GTATCAACGCAGAGTACTTTTTTTT 1529 0.24340544756992535 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1290 0.20535842208319402 No Hit CTTTAGGACGTGAAATATGGCGAGG 1241 0.19755798589553786 No Hit GTCCTACAGTGTGCATTTCTCATTT 1180 0.18784723880478216 No Hit GTACATGGGAAGCAGTGGTATCAAC 1042 0.16587866341913812 No Hit GGTATCAACGCAGAGTACTTTTTTT 1019 0.16221723418819747 No Hit CCCATGTACTCTGCGTTGATACCAC 936 0.14900425044175938 No Hit CTGTAGGACCTGGAATATGGCGAGA 899 0.14311412515720265 No Hit TATCAACGCAGAGTACTTTTTTTTT 815 0.12974194883550633 No Hit CTGAAGGACCTGGAATATGGCGAGA 708 0.11270834328286929 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGGCTTA 40 0.0052086087 14.277006 6 GACAATC 40 0.0053115627 14.234955 7 TCCAACG 110 1.8735591E-10 13.805791 18 GTACTAA 70 7.0240185E-6 13.612363 1 GTGTAAG 70 7.0240185E-6 13.612363 1 GATATAC 225 0.0 13.551866 1 TAGGACC 340 0.0 13.15724 4 CCAACGA 80 2.0141833E-6 13.050786 19 GGCGAGG 360 0.0 12.655308 19 TATTTAG 55 0.0030001511 12.12544 2 AGGACGT 890 0.0 11.763824 5 TCTTAGG 65 7.82226E-4 11.7257 2 CTGTAGG 1025 0.0 11.713273 1 TTAGGAC 480 0.0 11.700149 3 GACGTGA 430 0.0 11.696939 7 GGACGTG 880 0.0 11.681187 6 ACTGTTC 255 0.0 11.535907 8 TGTAGGA 1045 0.0 11.487259 2 TGGCGAG 910 0.0 11.160313 18 GGACCTG 590 0.0 11.131226 6 >>END_MODULE