FastQCFastQC Report
Thu 26 May 2016
SRR1926909_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926909_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences636494
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT15180.23849399994344014No Hit
CTGTAGGACGTGGAATATGGCAAGA11650.18303393276291685No Hit
GTCCTACAGTGTGCATTTCTCATTT11130.17486417782414287No Hit
GTCCTAAAGTGTGTATTTCTCATTT10910.1714077430423539No Hit
CTTTAGGACGTGAAATATGGCGAGG9540.14988358099212248No Hit
GTATCAACGCAGAGTACTTTTTTTT8580.13480095648977053No Hit
CTGTAGGACCTGGAATATGGCGAGA8440.13260140708317752No Hit
GTACATGGGAAGCAGTGGTATCAAC7400.1162618972056296No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTC307.6172577E-419.0363645
TAGGACC2800.014.96540454
AATCCCG1250.014.41306719
ACTGTTC2250.014.3716948
TAAGACC551.9167803E-413.8522754
ATAAGAC500.001457208913.3486183
AGGACCT5250.012.8722075
TCTAAAC603.9622094E-412.7129693
GATATAC2300.012.43954851
AAATCCC1457.2759576E-1212.42505718
GGACCTG4800.012.2943176
GTATAAG550.002977068512.1379831
AATGCCG550.00310817712.068356518
GTCCTAA4250.011.8931841
TGTAGGA8600.011.8629732
CACTGTT2350.011.7458417
CTTAGAC657.770349E-411.7350483
TTGGACC657.847983E-411.7211564
CTGTAGG8150.011.7018241
ATTTAGA1800.011.6563181
Produced by FastQC (version 0.11.5)