##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926909_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 636494 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.908250509824132 31.0 28.0 31.0 16.0 34.0 2 28.10742913523144 31.0 28.0 31.0 16.0 34.0 3 27.5681954582447 31.0 27.0 31.0 16.0 34.0 4 31.282725367403305 35.0 32.0 37.0 17.0 37.0 5 31.187931072406023 35.0 32.0 37.0 17.0 37.0 6 30.954455187323052 35.0 32.0 37.0 11.0 37.0 7 31.051675271094464 35.0 32.0 37.0 13.0 37.0 8 30.64840674067627 35.0 31.0 37.0 10.0 37.0 9 32.85164667695218 37.0 32.0 39.0 17.0 39.0 10 33.01050913284336 37.0 33.0 39.0 17.0 39.0 11 33.18421226280216 37.0 33.0 39.0 17.0 39.0 12 33.04313316386329 37.0 33.0 39.0 17.0 39.0 13 33.15276970403492 38.0 33.0 39.0 17.0 39.0 14 34.136027990837306 39.0 34.0 40.0 17.0 41.0 15 33.70231298331171 39.0 33.0 40.0 10.0 41.0 16 33.77980625111941 39.0 33.0 40.0 11.0 41.0 17 33.99792456802421 39.0 33.0 40.0 16.0 41.0 18 34.04763438461321 39.0 34.0 41.0 11.0 41.0 19 33.4165192444862 39.0 33.0 40.0 10.0 41.0 20 33.71194072528571 39.0 33.0 40.0 10.0 41.0 21 33.69922418750216 39.0 33.0 40.0 8.0 41.0 22 33.71846867370313 39.0 34.0 41.0 2.0 41.0 23 33.70108594896417 39.0 34.0 41.0 2.0 41.0 24 32.402126335833486 38.0 32.0 40.0 2.0 41.0 25 32.957825211235296 39.0 33.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.45358822998326787 1101 2 -0.6344676447352491 1101 3 -1.3968008293640999 1101 4 -0.6859935567243625 1101 5 -0.5875397207548261 1101 6 -2.1220666634451746 1101 7 -0.7390633676457732 1101 8 -0.27060846537811045 1101 9 -0.4514918143126607 1101 10 -9.405867986197396 1101 11 -4.44146376349725 1101 12 -1.8913056435045945 1101 13 -1.1051952438131991 1101 14 -0.8065103005609302 1101 15 -2.193798427037777 1101 16 -0.6112737765346026 1101 17 -0.48284108116338587 1101 18 -0.3477562901692721 1101 19 -1.1502167480214496 1101 20 -0.4916309307852842 1101 21 -0.6773558764653913 1101 22 -0.4279301535658817 1101 23 -0.5508214884871023 1101 24 -0.48015876565055393 1101 25 -0.4125452165724752 1102 1 -0.324903930488631 1102 2 -0.3292091894517668 1102 3 -0.810015351859569 1102 4 -0.6247462624589808 1102 5 -0.30274935207241427 1102 6 -1.227554090834893 1102 7 -0.30015479403442313 1102 8 -0.056767617456859654 1102 9 0.08785714093350094 1102 10 -0.04917642457689908 1102 11 -0.49728861191024976 1102 12 -0.49929683587189544 1102 13 -0.5356595105536286 1102 14 -0.859304506666561 1102 15 -0.05011678388301277 1102 16 -0.36568966715486795 1102 17 -0.5522110675962963 1102 18 -0.6384147551121728 1102 19 -0.4026359930940444 1102 20 -0.4626471893415527 1102 21 -0.42136813514459703 1102 22 -0.5141382613298546 1102 23 -0.9554402268793396 1102 24 -4.4712929569158035 1102 25 -2.746511900362659 1103 1 -0.25106426804137527 1103 2 -0.48321762827033155 1103 3 -0.27309552063593756 1103 4 -0.1321302287037085 1103 5 -0.5896691832960421 1103 6 0.32940793448156214 1103 7 0.36862157727359346 1103 8 0.3250889226275291 1103 9 0.47498794262126154 1103 10 0.3664353897690944 1103 11 0.048070037878773064 1103 12 -0.04254578101957662 1103 13 0.04978352742105585 1103 14 -0.0850429032910327 1103 15 0.16190853257268856 1103 16 0.3083609657565276 1103 17 -0.017400941014017235 1103 18 -0.1320856411881266 1103 19 -1.4089651070180977 1103 20 -0.5997779910293204 1103 21 -0.29900526594628474 1103 22 -0.2831256031020075 1103 23 -0.2486891720270208 1103 24 1.2755424861221698 1103 25 0.437032403434813 1104 1 -0.7433478903510569 1104 2 -0.7348663890404623 1104 3 -1.973929651756741 1104 4 -1.2431556515310653 1104 5 -0.9073658822287811 1104 6 -2.3346570586042787 1104 7 -0.7676050652821651 1104 8 -0.27978024022748826 1104 9 -0.3951145287470297 1104 10 -0.2528870227402109 1104 11 -0.41417688620731496 1104 12 -0.32217028905063927 1104 13 -0.6034758776067761 1104 14 -0.4026997128502998 1104 15 -0.00788280168253408 1104 16 -0.5356981768878697 1104 17 -0.5248469573952548 1104 18 -0.6444513469641038 1104 19 -1.9185296923458992 1104 20 -0.7664136326277315 1104 21 -0.44472114723550504 1104 22 -0.39120807659773504 1104 23 -0.3137664687684989 1104 24 0.8429377986775748 1104 25 0.3197266203620899 1105 1 0.009882730333732326 1105 2 -0.07630474822463995 1105 3 0.43152922644301484 1105 4 0.010713157272622453 1105 5 0.0576980027165952 1105 6 -0.4255630432436739 1105 7 0.12611401731414773 1105 8 0.36937611696386696 1105 9 -0.6264516596119662 1105 10 -0.08791263844294406 1105 11 -0.12016064382368796 1105 12 0.009654466035030396 1105 13 0.0758814371974239 1105 14 -0.23563220379001848 1105 15 0.423659122537309 1105 16 -0.11103082831150601 1105 17 -0.1770134903253222 1105 18 -0.024756354591502827 1105 19 -0.6850740855491395 1105 20 -0.03188335671883635 1105 21 -0.06201880081950151 1105 22 0.04492461362421096 1105 23 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2216 12 -2.2641681256492525 2216 13 -2.0645235531611483 2216 14 -2.14701775707087 2216 15 -3.0337316138298718 2216 16 -2.2042759826499463 2216 17 -2.5237026956559774 2216 18 -2.161578947283772 2216 19 -1.4872096138822357 2216 20 -2.294123131412835 2216 21 -5.078088182810546 2216 22 -3.491624612088014 2216 23 -3.5680175928492552 2216 24 -3.542922597354039 2216 25 -3.2286219697944354 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 39735.0 3 8.0 4 297.0 5 1707.0 6 2149.0 7 1873.0 8 1382.0 9 1296.0 10 1255.0 11 1241.0 12 1251.0 13 1223.0 14 1225.0 15 1246.0 16 1329.0 17 1474.0 18 1729.0 19 2091.0 20 2636.0 21 3658.0 22 4443.0 23 5259.0 24 6202.0 25 7089.0 26 7932.0 27 8753.0 28 9819.0 29 11359.0 30 13761.0 31 16738.0 32 21338.0 33 28010.0 34 37758.0 35 52050.0 36 74727.0 37 116052.0 38 146399.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.07039033667955 18.78865265199703 12.657129721763882 25.483827289559535 2 13.836230630063714 19.70518367025879 43.20994257846299 23.248643121214503 3 18.94473697872829 24.970541177062866 29.188226539528312 26.896495304680528 4 11.908898108568275 16.738015246533845 38.76546781138821 32.58761883350967 5 12.42602341478746 38.05248683900369 36.283806081558886 13.237683664649957 6 32.048608546568 37.26492852192743 18.288706354531868 12.397756576972698 7 27.623055821230878 31.00932638568284 23.009969258694937 18.357648534391345 8 24.264410464868952 37.88402303468595 20.7580018749163 17.0935646255288 9 26.223621829473153 15.454398312928506 20.225686669212358 38.096293188385985 10 14.453121930289175 29.60203188334274 34.897847882223694 21.04699830414439 11 34.236817790404594 22.472296774923475 24.693740296800065 18.597145137871866 12 23.125684537044748 24.962797326750255 32.15458083940545 19.75693729679955 13 29.08058963194358 22.359156312804743 25.529347247080047 23.03090680817163 14 20.255913461462917 22.783777795586648 27.594058332429793 29.366250410520646 15 22.81028980337745 32.037863157745875 22.801018860868293 22.35082817800838 16 21.50476298230892 28.4199658686982 28.82083580832414 21.254435340668735 17 21.17946920899915 29.244261602115746 28.21483065695954 21.361438531925558 18 21.030086754568327 27.99276547667872 30.33220038561078 20.644947383142178 19 23.729482112409343 26.956555902158453 27.245868921244316 22.068093064187888 20 23.309343474024953 27.907696659228765 28.324428800402274 20.45853106634401 21 22.509047082541763 27.001396935541404 27.249199786924116 23.240356194992717 22 22.48647816235005 28.84606318885945 27.439666361816407 21.227792286974097 23 21.721626399528578 28.39237870752308 27.972500491062657 21.91349440188568 24 23.28278701404821 27.23058549923002 28.34045695967818 21.14617052704359 25 22.312924956276742 27.733980317070216 28.296372187451386 21.65672253920165 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 186.0 1 186.0 2 249.5 3 313.0 4 313.0 5 313.0 6 458.5 7 604.0 8 604.0 9 604.0 10 945.0 11 1286.0 12 1286.0 13 1286.0 14 2162.0 15 3038.0 16 3038.0 17 3038.0 18 5185.5 19 7333.0 20 7333.0 21 7333.0 22 11753.0 23 16173.0 24 16173.0 25 16173.0 26 23398.0 27 30623.0 28 30623.0 29 30623.0 30 40694.5 31 50766.0 32 50766.0 33 50766.0 34 57649.5 35 64533.0 36 64533.0 37 64533.0 38 69130.5 39 73728.0 40 73728.0 41 73728.0 42 77006.0 43 80284.0 44 80284.0 45 80284.0 46 82300.5 47 84317.0 48 84317.0 49 84317.0 50 80249.5 51 76182.0 52 76182.0 53 76182.0 54 66944.0 55 57706.0 56 57706.0 57 57706.0 58 49050.0 59 40394.0 60 40394.0 61 40394.0 62 32850.5 63 25307.0 64 25307.0 65 25307.0 66 19527.0 67 13747.0 68 13747.0 69 13747.0 70 9963.0 71 6179.0 72 6179.0 73 6179.0 74 4321.0 75 2463.0 76 2463.0 77 2463.0 78 1692.5 79 922.0 80 922.0 81 922.0 82 602.5 83 283.0 84 283.0 85 283.0 86 190.5 87 98.0 88 98.0 89 98.0 90 62.0 91 26.0 92 26.0 93 26.0 94 14.0 95 2.0 96 2.0 97 2.0 98 1.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.21209940706432423 2 0.13260140708317752 3 0.13527228850546902 4 0.06520092883829227 5 0.022623936753527918 6 0.02278104742542742 7 0.2895549683107775 8 0.2826420987471995 9 0.019638833987437432 10 0.03723522924018137 11 0.01665373122134694 12 0.018067727268442437 13 0.015239735174251446 14 0.016810841893246442 15 0.015239735174251446 16 0.020110166003135928 17 0.019324612643638432 18 0.015868177861849443 19 0.02498059683202041 20 0.017910616596542937 21 0.015868177861849443 22 0.018224837940341937 23 0.018696169956040433 24 0.017910616596542937 25 0.016496620549447442 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 636494.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 84.96596573182954 #Duplication Level Percentage of deduplicated Percentage of total 1 93.52910112764245 79.46790401340087 2 4.866152554770952 8.269147024290472 3 0.7830258392505531 1.9959163987469868 4 0.2648218273455574 0.9000336922913243 5 0.14396099238198495 0.6115892372723953 6 0.09503824976429268 0.48450100036115695 7 0.056793627919419803 0.337786781051139 8 0.04465287490095758 0.30351797109299467 9 0.026267235569192263 0.20086389335176952 >10 0.15839292435429464 2.533909973800356 >50 0.01645099830712118 0.9734142186207856 >100 0.013493344445261696 2.437280297114101 >500 0.0011090420087227556 0.716625426469599 >1k 7.393613391485037E-4 0.7675100721360794 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 1518 0.23849399994344014 No Hit CTGTAGGACGTGGAATATGGCAAGA 1165 0.18303393276291685 No Hit GTCCTACAGTGTGCATTTCTCATTT 1113 0.17486417782414287 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1091 0.1714077430423539 No Hit CTTTAGGACGTGAAATATGGCGAGG 954 0.14988358099212248 No Hit GTATCAACGCAGAGTACTTTTTTTT 858 0.13480095648977053 No Hit CTGTAGGACCTGGAATATGGCGAGA 844 0.13260140708317752 No Hit GTACATGGGAAGCAGTGGTATCAAC 740 0.1162618972056296 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 1.5711067189949944E-4 1.5711067189949944E-4 0.0 0.0 0.0 11 1.5711067189949944E-4 1.5711067189949944E-4 0.0 0.0 0.0 12 1.5711067189949944E-4 1.5711067189949944E-4 0.0 0.0 0.0 13 1.5711067189949944E-4 1.5711067189949944E-4 0.0 1.5711067189949944E-4 1.5711067189949944E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTCGTC 30 7.6172577E-4 19.036364 5 TAGGACC 280 0.0 14.9654045 4 AATCCCG 125 0.0 14.413067 19 ACTGTTC 225 0.0 14.371694 8 TAAGACC 55 1.9167803E-4 13.852275 4 ATAAGAC 50 0.0014572089 13.348618 3 AGGACCT 525 0.0 12.872207 5 TCTAAAC 60 3.9622094E-4 12.712969 3 GATATAC 230 0.0 12.4395485 1 AAATCCC 145 7.2759576E-12 12.425057 18 GGACCTG 480 0.0 12.294317 6 GTATAAG 55 0.0029770685 12.137983 1 AATGCCG 55 0.003108177 12.0683565 18 GTCCTAA 425 0.0 11.893184 1 TGTAGGA 860 0.0 11.862973 2 CACTGTT 235 0.0 11.745841 7 CTTAGAC 65 7.770349E-4 11.735048 3 TTGGACC 65 7.847983E-4 11.721156 4 CTGTAGG 815 0.0 11.701824 1 ATTTAGA 180 0.0 11.656318 1 >>END_MODULE