##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926909_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 636494 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.88503740805098 31.0 30.0 33.0 26.0 34.0 2 30.18410856975871 31.0 30.0 34.0 26.0 34.0 3 30.251105273576812 31.0 30.0 34.0 26.0 34.0 4 33.855748836595474 35.0 35.0 37.0 29.0 37.0 5 33.85022796758493 37.0 35.0 37.0 30.0 37.0 6 33.8053712996509 37.0 35.0 37.0 30.0 37.0 7 33.79731937771605 37.0 35.0 37.0 29.0 37.0 8 33.81933529616933 37.0 35.0 37.0 29.0 37.0 9 35.353778668769856 39.0 35.0 39.0 30.0 39.0 10 35.23928583772982 39.0 35.0 39.0 29.0 39.0 11 35.28332710127668 39.0 35.0 39.0 30.0 39.0 12 35.186118015252305 39.0 35.0 39.0 29.0 39.0 13 35.20198147979399 39.0 35.0 39.0 29.0 39.0 14 36.2864300370467 40.0 36.0 41.0 29.0 41.0 15 36.27725477380777 40.0 36.0 41.0 29.0 41.0 16 36.26162697527392 40.0 36.0 41.0 29.0 41.0 17 36.23197390705961 40.0 36.0 41.0 29.0 41.0 18 36.20489902497117 40.0 36.0 41.0 29.0 41.0 19 36.26957520416532 40.0 37.0 41.0 29.0 41.0 20 36.2289542399457 40.0 37.0 41.0 29.0 41.0 21 36.168790279248505 40.0 37.0 41.0 29.0 41.0 22 36.08604008835904 40.0 37.0 41.0 29.0 41.0 23 36.00994510553124 40.0 36.0 41.0 28.0 41.0 24 35.940508472978536 40.0 36.0 41.0 28.0 41.0 25 35.826937881582545 40.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.008759167599933448 1101 2 0.0939388662733407 1101 3 0.21398764143647853 1101 4 0.15185212102786494 1101 5 0.08878283237680762 1101 6 0.22992237241769686 1101 7 0.2067394951549133 1101 8 0.3163616562211189 1101 9 0.28308362413447696 1101 10 0.44005816607543125 1101 11 0.1393223319319432 1101 12 0.32726917745090134 1101 13 0.45638210174374194 1101 14 0.2925066966735699 1101 15 0.2486190036722178 1101 16 0.3992712189010845 1101 17 0.4506924643800687 1101 18 0.4480047464854735 1101 19 0.33287125248040184 1101 20 0.2993005766562007 1101 21 0.35046356600916795 1101 22 0.1818656421603535 1101 23 0.491388934247702 1101 24 0.31391020604844755 1101 25 0.22333167378976526 1102 1 -0.7678554851664607 1102 2 -0.7458761558774576 1102 3 -0.442009759302028 1102 4 -0.7278519056321215 1102 5 -0.7969802835757207 1102 6 -0.5673033989087628 1102 7 -0.5474656445334389 1102 8 -0.6437943433749425 1102 9 -0.7599139566204514 1102 10 -0.8167605047008735 1102 11 -0.9013405236553709 1102 12 -0.8957393360086243 1102 13 -0.7297614635473337 1102 14 -0.5076761736663116 1102 15 -0.6237219123040205 1102 16 -0.6517085534000984 1102 17 -0.8793665458808633 1102 18 -0.9606698496850044 1102 19 -0.8678701078290487 1102 20 -0.8894817659282168 1102 21 -0.9449872873125784 1102 22 -0.693942785242541 1102 23 -0.7335295095322891 1102 24 -0.7818354790457605 1102 25 -0.8073578330091493 1103 1 -0.9830453585841816 1103 2 -1.0085343837255607 1103 3 -0.8839928816648985 1103 4 -1.1044341841131384 1103 5 -1.1524655156432289 1103 6 -1.2751093060817595 1103 7 -1.252106994744409 1103 8 -0.9380981408433016 1103 9 -1.1544287245529432 1103 10 -0.9718237958401161 1103 11 -1.0237033928536832 1103 12 -1.0508026271478599 1103 13 -1.0979049234629414 1103 14 -1.1690685787296005 1103 15 -1.315705034666891 1103 16 -1.0694300723874406 1103 17 -0.8171302589610363 1103 18 -1.1125685838622204 1103 19 -1.0556338209092218 1103 20 -1.1088910486286423 1103 21 -1.2951982577767112 1103 22 -1.221368945580089 1103 23 -1.2472425897010666 1103 24 -1.2755063651217071 1103 25 -1.3337291410260264 1104 1 -0.4469144505247584 1104 2 -0.4274171266961666 1104 3 -0.2069860029640509 1104 4 -0.242025929672117 1104 5 -0.1312665297197455 1104 6 -0.2163389625012897 1104 7 -0.21972125563863898 1104 8 -0.1539882943730646 1104 9 -0.1930682309884304 1104 10 -0.22810415608547885 1104 11 -0.05419217064328308 1104 12 -0.05554503043829584 1104 13 0.08023658630551722 1104 14 -0.2997816234411559 1104 15 -0.2725358682661536 1104 16 -0.17215407338024136 1104 17 -0.16033399615211152 1104 18 -0.2589936095687051 1104 19 -0.3064892263625367 1104 20 -0.25440748305051386 1104 21 -0.27055992142951624 1104 22 -0.12424275333103907 1104 23 -0.10452284357477026 1104 24 -0.004116796636083109 1104 25 -0.12895039408420672 1105 1 0.006981693452246418 1105 2 -0.20787380044646397 1105 3 -0.09266215170266534 1105 4 0.10675765511541613 1105 5 -0.03444159827812143 1105 6 -0.28199628524150455 1105 7 -0.33050255661652983 1105 8 -0.6646556253138343 1105 9 -0.5615441628092128 1105 10 -0.4484487997141784 1105 11 -0.446874059768291 1105 12 -0.23930573304042468 1105 13 -0.4016946436891047 1105 14 -0.3186473019266103 1105 15 -0.4163122665484451 1105 16 -0.4041536413081488 1105 17 -0.6861359748659481 1105 18 -0.5275694029500713 1105 19 -0.4963661739936356 1105 20 -0.5482032804880603 1105 21 -0.4981682260315239 1105 22 -0.31939605074654054 1105 23 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2211 14 -0.2551278998827655 2211 15 -0.1466158450844688 2211 16 -0.24311176680243562 2211 17 -0.2703093656102382 2211 18 -0.17822716505987302 2211 19 -0.20601834835616728 2211 20 -0.35805859866917444 2211 21 -0.3192052416265625 2211 22 -0.3890790326703595 2211 23 -0.19940113972081264 2211 24 -0.3407003955929113 2211 25 -0.21486027527806328 2212 1 -0.04408341519800629 2212 2 -0.07155924956473214 2212 3 0.03296463748630529 2212 4 0.017662411683836865 2212 5 -0.030893273621188655 2212 6 -0.14116559218228275 2212 7 -0.10206807786267547 2212 8 -0.39762458384031163 2212 9 -0.08267009114996426 2212 10 0.003838815277823926 2212 11 -0.03634318638938794 2212 12 -0.05231014425081071 2212 13 -0.2749828801218257 2212 14 -0.21178292881767646 2212 15 -0.15260847983505244 2212 16 -0.0910432795481455 2212 17 -0.1321439822824857 2212 18 -0.07288975054937197 2212 19 -0.20970575569271688 2212 20 -0.06510341190749358 2212 21 -0.14341012947389942 2212 22 -0.15290800583981223 2212 23 -0.38528281746724247 2212 24 -0.24643955098053993 2212 25 -0.33570775722363777 2213 1 0.14957156261855076 2213 2 0.20750663248245615 2213 3 0.2742369833624103 2213 4 0.1037972944550063 2213 5 0.052070954413487414 2213 6 0.29923055402424836 2213 7 0.2171711877631992 2213 8 0.22019828274343922 2213 9 0.17231329447070465 2213 10 0.07408144090666013 2213 11 -0.004697898747146212 2213 12 0.009797932750807092 2213 13 0.027307862234629 2213 14 0.09025163651674717 2213 15 0.09670188419620729 2213 16 0.017152528801744893 2213 17 -0.08709580986421628 2213 18 0.0999387779178349 2213 19 -0.051763339832021416 2213 20 -0.21216572975263404 2213 21 -0.06559543085317188 2213 22 -0.4284709455639586 2213 23 -0.20871930227297497 2213 24 -0.21872788007519262 2213 25 -0.0968653803464008 2214 1 0.39493873687496617 2214 2 0.1811157004233408 2214 3 0.2058370657719486 2214 4 0.22245438041032628 2214 5 0.3319290509699968 2214 6 0.17631564187354343 2214 7 0.11968757027357668 2214 8 0.11104720943148294 2214 9 0.11534227877313441 2214 10 -0.11825085748815667 2214 11 -0.19969224212745473 2214 12 0.056800285760019165 2214 13 -0.032928812179015665 2214 14 -0.11053125410627729 2214 15 -0.20637726872047324 2214 16 -0.09382926519609924 2214 17 -0.08799400155314174 2214 18 0.12286887077314645 2214 19 0.28425794207004884 2214 20 0.0842845154880365 2214 21 0.1476482881302985 2214 22 -0.013652770646096712 2214 23 -0.09988630086694883 2214 24 -0.21928812036406242 2214 25 -0.14033821953574943 2215 1 0.45936657351276367 2215 2 0.34434953345626695 2215 3 0.31108610960190575 2215 4 0.33849781470935625 2215 5 0.3614977853085932 2215 6 0.27920921025619094 2215 7 0.2568617031262619 2215 8 0.17911508651417307 2215 9 0.014950139150329278 2215 10 0.2653109308795365 2215 11 0.18229307461761124 2215 12 0.10236585484605598 2215 13 0.14023951771063992 2215 14 0.27750584968762837 2215 15 0.37460308033654144 2215 16 0.21132745876750647 2215 17 0.3168919174691496 2215 18 0.09525204218036265 2215 19 0.1621750300376945 2215 20 0.11819755896629403 2215 21 0.0568653277399207 2215 22 0.031408995740683565 2215 23 0.23517326742776845 2215 24 0.05590860887495808 2215 25 0.055938212521219555 2216 1 0.22273900163420635 2216 2 0.3871902724594598 2216 3 -1.7030416298257478 2216 4 -0.9809526242692641 2216 5 -0.05499351330856683 2216 6 0.13538328259271282 2216 7 0.25661149648238535 2216 8 0.3644833594181378 2216 9 0.36790860931542113 2216 10 0.2186089672041831 2216 11 0.3136274355853743 2216 12 0.3123401639852972 2216 13 0.37230081833838824 2216 14 0.2918319672400713 2216 15 0.29741343710441015 2216 16 0.5017101408933655 2216 17 0.2316483010895496 2216 18 0.3992263066398536 2216 19 0.282566424716336 2216 20 0.5084377126646586 2216 21 0.5651234873806956 2216 22 0.7198329172830285 2216 23 0.5752443082427305 2216 24 0.6521465732651919 2216 25 0.6685021604246231 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 14944.0 3 16.0 4 214.0 5 1612.0 6 2191.0 7 1558.0 8 1271.0 9 1049.0 10 967.0 11 891.0 12 798.0 13 787.0 14 770.0 15 813.0 16 872.0 17 878.0 18 997.0 19 1174.0 20 1459.0 21 1902.0 22 2427.0 23 3039.0 24 3606.0 25 4190.0 26 5200.0 27 5596.0 28 6618.0 29 7806.0 30 10246.0 31 12857.0 32 17136.0 33 22512.0 34 30302.0 35 42670.0 36 62728.0 37 108025.0 38 256373.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.68700964508843 19.428907582058752 10.640740903857408 28.243341868995415 2 13.892943197045668 20.79057960529213 40.68419595729556 24.63228124036665 3 18.611839169322245 24.72799954745159 29.254740758898546 27.405420524327617 4 11.888250321291324 17.442898126298125 38.036650777540714 32.63220077486984 5 12.712189297049303 38.73739119504761 35.460987336203374 13.089432171699714 6 31.63998757621002 38.01222513547134 17.758609544560887 12.589177743757753 7 28.04936688571886 31.840352746323724 21.601469566829778 18.50881080112763 8 24.833329405202416 38.74551211042762 19.645211215599392 16.775947268770576 9 26.185582884742896 15.712135353325854 19.867135526162798 38.23514623576845 10 14.847390731889728 29.93705830041699 33.99024607910606 21.22530488858722 11 34.095144123959486 22.799175446920966 24.47797682829876 18.627703600820784 12 23.39290988731987 25.17062272581588 31.684765872941057 19.75170151392319 13 29.37346618778732 22.2480934380332 25.486656422922106 22.891783951257374 14 20.41338959990196 23.035095381888908 27.258858685235055 29.292656332974076 15 22.625655754060215 31.974245892650373 22.966435761633104 22.43366259165631 16 21.606746992494788 28.61941488567788 28.470473266707618 21.30336485511971 17 21.11067992892302 29.44541416794843 28.035343672279193 21.408562230849356 18 21.021854231802543 28.100834262910652 30.248707756602617 20.628603748684192 19 23.356145874575017 27.103247173570132 27.388246827925567 22.152360123929288 20 23.28328287438471 27.848783407777955 28.31273340864207 20.55520030919526 21 22.45070803190386 26.952351621276783 27.201875514603746 23.39506483221561 22 22.713332725838736 28.834207392371336 27.173704701065525 21.278755180724406 23 21.77632467863012 28.47520950708098 27.78188011198849 21.966585702300414 24 22.946871288248712 27.25297411436436 28.562181457111794 21.23797314027513 25 22.3501000851588 27.89264963281159 28.12031587316052 21.636934408869084 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 236.0 1 236.0 2 244.5 3 253.0 4 253.0 5 253.0 6 400.5 7 548.0 8 548.0 9 548.0 10 861.5 11 1175.0 12 1175.0 13 1175.0 14 1980.0 15 2785.0 16 2785.0 17 2785.0 18 4930.0 19 7075.0 20 7075.0 21 7075.0 22 11213.0 23 15351.0 24 15351.0 25 15351.0 26 22596.5 27 29842.0 28 29842.0 29 29842.0 30 39918.0 31 49994.0 32 49994.0 33 49994.0 34 56499.5 35 63005.0 36 63005.0 37 63005.0 38 67853.5 39 72702.0 40 72702.0 41 72702.0 42 76281.5 43 79861.0 44 79861.0 45 79861.0 46 83036.5 47 86212.0 48 86212.0 49 86212.0 50 82041.5 51 77871.0 52 77871.0 53 77871.0 54 68046.5 55 58222.0 56 58222.0 57 58222.0 58 49932.0 59 41642.0 60 41642.0 61 41642.0 62 33798.5 63 25955.0 64 25955.0 65 25955.0 66 19900.5 67 13846.0 68 13846.0 69 13846.0 70 10032.5 71 6219.0 72 6219.0 73 6219.0 74 4328.5 75 2438.0 76 2438.0 77 2438.0 78 1670.0 79 902.0 80 902.0 81 902.0 82 583.5 83 265.0 84 265.0 85 265.0 86 168.0 87 71.0 88 71.0 89 71.0 90 45.5 91 20.0 92 20.0 93 20.0 94 12.0 95 4.0 96 4.0 97 4.0 98 2.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.11469079048663458 2 0.10714947823545862 3 0.015396845846150947 4 0.0 5 0.005341762844582982 6 0.3498854663201853 7 0.018224837940341937 8 0.009269529642070468 9 0.008798197626371969 10 0.005027541500783982 11 0.004399098813185984 12 3.1422134379899887E-4 13 0.0012568853751959955 14 0.0 15 0.002042438734693493 16 4.7133201569849836E-4 17 1.5711067189949944E-4 18 6.284426875979977E-4 19 3.1422134379899887E-4 20 4.7133201569849836E-4 21 0.012883075095758954 22 0.0 23 0.0 24 3.1422134379899887E-4 25 0.0056559841883819795 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 636494.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 82.18747495393607 #Duplication Level Percentage of deduplicated Percentage of total 1 91.93101731459393 75.55578183033053 2 6.1017080998705095 10.02967963268673 3 0.9828914289557537 2.423440940992184 4 0.34989412547600135 1.1502765869635292 5 0.16886270232789877 0.6939199559114072 6 0.10175625497171904 0.5017853794136696 7 0.05742732558396312 0.3303864818172451 8 0.045810121766502454 0.3012014588256946 9 0.03743825127066451 0.2769259804764215 >10 0.1862792829146116 2.8556170006425745 >50 0.018935810362826527 1.1035461243604445 >100 0.015492785472464346 2.673328330926426 >500 0.0011476137384090306 0.6894753650445045 >1k 0.0013388826948105357 1.4146349316086402 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 1571 0.24682086555411362 No Hit GTATCAACGCAGAGTACTTTTTTTT 1470 0.23095268769226418 No Hit CTGTAGGACGTGGAATATGGCAAGA 1405 0.2207404940187967 No Hit GTCCTACAGTGTGCATTTCTCATTT 1198 0.1882185849356003 No Hit CTTTAGGACGTGAAATATGGCGAGG 1179 0.18523348216950986 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1149 0.18052016201252485 No Hit CTGTAGGACCTGGAATATGGCGAGA 1027 0.16135266004078594 No Hit GTACATGGGAAGCAGTGGTATCAAC 872 0.1370005058963635 No Hit GGTATCAACGCAGAGTACTTTTTTT 855 0.134329624474072 No Hit TATCAACGCAGAGTACTTTTTTTTT 805 0.12647409087909706 No Hit CCCATGTACTCTGCGTTGATACCAC 751 0.11799011459652409 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGACCGT 50 8.5168016E-5 15.2381735 6 ATGTCGA 40 0.005316437 14.2330265 17 TGTCGAG 40 0.005316437 14.2330265 18 GACGAAA 40 0.005324758 14.229673 11 TAGGACC 355 0.0 13.95044 4 GTATTAG 50 0.0014576033 13.348142 1 CCCCAGT 50 0.0014576033 13.348142 1 GACGTGG 320 0.0 13.342415 7 GTAGGAC 810 0.0 13.168792 3 TGTAGGA 895 0.0 12.783534 2 CCTTACA 75 1.4215089E-5 12.712514 2 ATAGGAC 90 5.226648E-7 12.698478 3 CTGTAGG 870 0.0 12.602924 1 GGACGTG 725 0.0 12.479538 6 AGGACGT 740 0.0 12.226575 5 TAGGACG 760 0.0 12.155451 4 CTAAACT 55 0.0030079598 12.121274 4 AACTGCC 55 0.0030943227 12.075558 7 TAGGACT 95 1.0056083E-6 12.030137 4 TAGCACT 105 2.62311E-7 11.791444 4 >>END_MODULE