FastQCFastQC Report
Thu 26 May 2016
SRR1926908_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926908_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences719517
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT16310.22667984217190143No Hit
GTCCTAAAGTGTGTATTTCTCATTT12920.17956490256658286No Hit
GTCCTACAGTGTGCATTTCTCATTT12790.17775813497109866No Hit
CTGTAGGACGTGGAATATGGCAAGA12640.17567340313015536No Hit
CTTTAGGACGTGAAATATGGCGAGG10250.14245667579779211No Hit
GTATCAACGCAGAGTACTTTTTTTT8740.12147037526562957No Hit
CTGTAGGACCTGGAATATGGCGAGA8650.12021953616106361No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG653.4223976E-614.59201518
TAGGACC3100.012.589944
GAACAGT2000.012.37246
AGGCTTA550.003024347812.1128396
TGCCGGT550.00310103612.07241410
CCAACGA550.003102651312.07157619
CTATAGT1209.678843E-911.8990294
GGCGAGG3450.011.82164719
TGTAGGA7750.011.7997872
GGAGTGT907.296103E-611.6321716
GTAGGAC7700.011.505273
ATTATAC1001.862667E-611.4310433
AGGACCT5700.011.3538895
GTCCTAG1201.2097735E-711.1228331
GGACCTG5400.011.10343556
CGGTTTC600.00594079511.06561113
CTGTAGG8150.010.9961051
GGACGTG7100.010.9917316
AGGACGT7200.010.97125155
GATATAC2450.010.8958371
Produced by FastQC (version 0.11.5)