##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926908_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 719517 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.970425994104378 31.0 28.0 31.0 16.0 34.0 2 28.223249763382935 31.0 28.0 33.0 16.0 34.0 3 27.680382812358847 31.0 28.0 31.0 16.0 34.0 4 31.35163449925436 35.0 32.0 37.0 17.0 37.0 5 31.24776065054752 35.0 32.0 37.0 17.0 37.0 6 30.983059469060496 35.0 32.0 37.0 11.0 37.0 7 31.08575474936659 35.0 32.0 37.0 11.0 37.0 8 30.71113677647644 35.0 31.0 37.0 10.0 37.0 9 32.881627536250015 37.0 33.0 39.0 17.0 39.0 10 33.03750849528225 37.0 33.0 39.0 17.0 39.0 11 33.190955877345495 38.0 33.0 39.0 17.0 39.0 12 33.063493982768996 38.0 33.0 39.0 16.0 39.0 13 33.17896866926007 38.0 33.0 39.0 17.0 39.0 14 34.182247257535266 39.0 34.0 41.0 17.0 41.0 15 33.75934133592396 39.0 33.0 41.0 10.0 41.0 16 33.83866538247185 39.0 33.0 40.0 11.0 41.0 17 34.061795621229244 39.0 34.0 41.0 16.0 41.0 18 34.103158090774784 39.0 34.0 41.0 11.0 41.0 19 33.45849229413621 39.0 33.0 41.0 10.0 41.0 20 33.75067302092932 39.0 33.0 41.0 10.0 41.0 21 33.750704986817546 39.0 34.0 41.0 8.0 41.0 22 33.783278226921674 39.0 34.0 41.0 2.0 41.0 23 33.76456706373859 39.0 34.0 41.0 2.0 41.0 24 32.457176133433954 38.0 32.0 40.0 2.0 41.0 25 33.040313154518934 39.0 33.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.48043635201990753 1101 2 -0.5603994610704035 1101 3 -1.4564618379834187 1101 4 -0.7629176528148314 1101 5 -0.7025567168887932 1101 6 -2.535807739242827 1101 7 -0.9384000083920192 1101 8 -0.3814487584319153 1101 9 -0.7485215208220595 1101 10 -9.370647923707395 1101 11 -4.0025059873571855 1101 12 -1.740107909872041 1101 13 -1.170304026558366 1101 14 -0.8358789046905528 1101 15 -1.919476662314537 1101 16 -0.8423749757357726 1101 17 -0.789981567088013 1101 18 -0.870424884666896 1101 19 -1.3378545316212183 1101 20 -0.6576476936846376 1101 21 -0.5997519632394344 1101 22 -0.40162796035875914 1101 23 -0.5525280152828174 1101 24 -0.6280610457844382 1101 25 -0.6548273027050584 1102 1 -0.1534558847124643 1102 2 -0.21097935347590635 1102 3 -0.47425535237093897 1102 4 -0.36468328046601783 1102 5 -0.2104715994630766 1102 6 -1.1359339980884293 1102 7 -0.0482177564931483 1102 8 0.44594841297070786 1102 9 0.46826192280062173 1102 10 0.369820282019667 1102 11 0.10831983532149536 1102 12 0.14474951245131962 1102 13 0.2231469820152583 1102 14 -0.024688278786030082 1102 15 0.41303573899894985 1102 16 0.2680565999546545 1102 17 -0.01252151578599836 1102 18 -0.23426744537594146 1102 19 0.3441453486075474 1102 20 0.47169775650549894 1102 21 0.2663587652691106 1102 22 0.1083030713706421 1102 23 -0.03396846402571896 1102 24 -3.6695642746173682 1102 25 -1.6670090355952354 1103 1 -0.4971015160419192 1103 2 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10 -0.31236811605742076 1107 11 -0.44278958787050726 1107 12 -0.2511228677118993 1107 13 -0.4158087660069185 1107 14 -0.39878157029404093 1107 15 -1.829046385937751 1107 16 -0.995345916140856 1107 17 -0.7333111988737073 1107 18 -0.9462169261764117 1107 19 0.19080392641513555 1107 20 0.3321600052083227 1107 21 -0.04512478409594678 1107 22 -0.22757858138713516 1107 23 -0.5478872203143084 1107 24 -4.657366866998057 1107 25 -2.6798690062649015 1108 1 0.0831735047437796 1108 2 -0.5119669406825764 1108 3 0.0935328664773536 1108 4 -0.11873135253528844 1108 5 0.16846159925810866 1108 6 -3.0160321096353044 1108 7 -1.5600578010187043 1108 8 -0.2442333134301009 1108 9 -0.274586806797231 1108 10 -0.09525822016131258 1108 11 -0.016215817482702732 1108 12 0.07809061187015942 1108 13 -0.006690318493419056 1108 14 -0.33385937859317494 1108 15 -1.433140758237613 1108 16 -0.2704599482792105 1108 17 -0.4594532648378902 1108 18 -0.30046038082682713 1108 19 -0.13252080032008706 1108 20 -1.345673035246918 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1110 8 1.4414142514800226 1110 9 1.359338527355483 1110 10 0.9820117137763162 1110 11 0.756134774006 1110 12 0.87525914774357 1110 13 0.6765774645761766 1110 14 0.3690304662370636 1110 15 1.0486321715519082 1110 16 0.6560817631972071 1110 17 0.38496215995854755 1110 18 0.6294290031031124 1110 19 -1.363115361696643 1110 20 0.361191306433831 1110 21 0.35649413714361344 1110 22 0.5022957453633126 1110 23 0.6179952244597118 1110 24 -0.27011372516681575 1110 25 -0.04672873618450524 1111 1 0.5583351708019073 1111 2 0.03456466982217066 1111 3 0.8396892427435994 1111 4 0.6855086283936984 1111 5 0.6553532958238186 1111 6 0.7080153204552531 1111 7 0.6462350295480235 1111 8 1.2124178530097467 1111 9 0.4191484259928444 1111 10 0.2514803953194047 1111 11 0.16577127940829683 1111 12 0.30036069603005444 1111 13 0.11896487036089809 1111 14 0.3708379143490461 1111 15 -0.7634132745550986 1111 16 -0.6774057799654543 1111 17 -0.27371307117054755 1111 18 0.03128546172265345 1111 19 0.5863251016754276 1111 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-2.113695147421147 2216 16 -1.3728300880069852 2216 17 -1.4924084783054994 2216 18 -1.5182222169906794 2216 19 -0.8482946002309362 2216 20 -1.8832503118795003 2216 21 -4.452021879071314 2216 22 -3.1043587336129193 2216 23 -3.1338941325071445 2216 24 -3.159876065203381 2216 25 -2.4262184428850837 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 45861.0 3 17.0 4 350.0 5 1857.0 6 2433.0 7 1968.0 8 1628.0 9 1545.0 10 1327.0 11 1369.0 12 1414.0 13 1351.0 14 1396.0 15 1380.0 16 1485.0 17 1668.0 18 1867.0 19 2297.0 20 2972.0 21 3887.0 22 4750.0 23 5711.0 24 6767.0 25 7745.0 26 8736.0 27 9565.0 28 10574.0 29 12712.0 30 14935.0 31 18397.0 32 23565.0 33 30719.0 34 41334.0 35 57271.0 36 83220.0 37 131085.0 38 174359.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.62405806668143 19.02207441882488 12.921006418585348 25.432861095908343 2 13.807003897547787 19.890017073513512 43.26598085039177 23.036998178546927 3 18.688624962602713 25.123045774280406 29.464331684373846 26.723997578743035 4 11.968356974274744 16.898950100205695 39.044200378014 32.08849254750557 5 12.477670782888417 38.07081124739174 36.20884994627087 13.242668023448982 6 31.66073081831299 37.534718457291085 18.414892167738454 12.389658556657464 7 27.28715658335714 31.3772897346033 23.31115307167592 18.02440061036364 8 24.111960181035826 37.849196263349164 21.020890464405948 17.017953091209062 9 25.967113484750126 15.63339695266638 20.624757604780765 37.77473195780273 10 14.431540588751353 29.588836164287574 35.00725059819173 20.972372648769344 11 33.72662458524407 22.779431164068896 25.06064089608131 18.43330335460572 12 23.109022922197973 24.85967277832608 32.38702233837001 19.644281961105936 13 28.715933511444224 22.58055996508211 25.839933722730823 22.86357280074284 14 20.083598836789047 22.942139707640383 27.86952242739662 29.10473902817395 15 22.57249771337861 32.11959377371761 23.199129276816024 22.10877923608775 16 21.12223195295884 28.596410748293643 29.068907516298985 21.21244978244853 17 20.9602422082318 29.38887738208265 28.398303541516945 21.252576868168607 18 20.82648740766688 28.254977022710403 30.485845266789546 20.43269030283317 19 23.48541718797787 27.197152945755832 27.434870853838238 21.88255901242806 20 23.025928948037848 28.15361369161886 28.468186196897644 20.352271163445646 21 22.416997379779122 27.29894843655521 27.247100034055922 23.03695414960975 22 22.317765999296626 29.021213776652754 27.749165613692696 20.911854610357928 23 21.65125295219084 28.507147116757324 28.109305083120073 21.73229484793176 24 23.164400185155067 27.299947038978477 28.514297390731404 21.021355385135053 25 22.168643548295176 27.96320261774064 28.434568299372113 21.43358553459207 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 185.0 1 185.0 2 259.0 3 333.0 4 333.0 5 333.0 6 509.5 7 686.0 8 686.0 9 686.0 10 1006.0 11 1326.0 12 1326.0 13 1326.0 14 2355.5 15 3385.0 16 3385.0 17 3385.0 18 6002.5 19 8620.0 20 8620.0 21 8620.0 22 13839.0 23 19058.0 24 19058.0 25 19058.0 26 27621.5 27 36185.0 28 36185.0 29 36185.0 30 48171.5 31 60158.0 32 60158.0 33 60158.0 34 67710.0 35 75262.0 36 75262.0 37 75262.0 38 80499.0 39 85736.0 40 85736.0 41 85736.0 42 88710.0 43 91684.0 44 91684.0 45 91684.0 46 93167.0 47 94650.0 48 94650.0 49 94650.0 50 89455.0 51 84260.0 52 84260.0 53 84260.0 54 73435.5 55 62611.0 56 62611.0 57 62611.0 58 53073.0 59 43535.0 60 43535.0 61 43535.0 62 35180.5 63 26826.0 64 26826.0 65 26826.0 66 20679.0 67 14532.0 68 14532.0 69 14532.0 70 10509.0 71 6486.0 72 6486.0 73 6486.0 74 4554.0 75 2622.0 76 2622.0 77 2622.0 78 1763.5 79 905.0 80 905.0 81 905.0 82 621.0 83 337.0 84 337.0 85 337.0 86 218.0 87 99.0 88 99.0 89 99.0 90 63.0 91 27.0 92 27.0 93 27.0 94 17.0 95 7.0 96 7.0 97 7.0 98 4.5 99 2.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2009681494669341 2 0.11952462554741584 3 0.12258223224746602 4 0.06893513287385844 5 0.023348996618564957 6 0.022098157513998976 7 0.2657338186589059 8 0.2597575873815351 9 0.018623604445760143 10 0.036969244646061175 11 0.01639989048208729 12 0.01765072958665327 13 0.01598294411389863 14 0.017928693832112375 15 0.01598294411389863 16 0.020430372041244337 17 0.01890156869121925 18 0.015427015622980416 19 0.024599835723130934 20 0.017789711709382823 21 0.015565997745709968 22 0.017511747463923713 23 0.01890156869121925 24 0.016677854727546397 25 0.01639989048208729 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 719517.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 84.89485148927074 #Duplication Level Percentage of deduplicated Percentage of total 1 93.28858152747203 79.19720274419464 2 5.0588451924864195 8.589398226466917 3 0.8227124202916865 2.095321462171236 4 0.2794704627687168 0.949024137295519 5 0.14864867922350056 0.6309753773377663 6 0.08575650177006745 0.4368171289205556 7 0.05754540808685801 0.3419716211396303 8 0.04188836537307214 0.28448852459802165 9 0.025034228519033447 0.19127494010446297 >10 0.16028491317398233 2.530541461177254 >50 0.016352323948344277 0.9756388026384437 >100 0.013408330826263212 2.4738552615226372 >500 6.540649155929555E-4 0.4024300762239621 >1k 8.175811444911944E-4 0.9010602362089473 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 1631 0.22667984217190143 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1292 0.17956490256658286 No Hit GTCCTACAGTGTGCATTTCTCATTT 1279 0.17775813497109866 No Hit CTGTAGGACGTGGAATATGGCAAGA 1264 0.17567340313015536 No Hit CTTTAGGACGTGAAATATGGCGAGG 1025 0.14245667579779211 No Hit GTATCAACGCAGAGTACTTTTTTTT 874 0.12147037526562957 No Hit CTGTAGGACCTGGAATATGGCGAGA 865 0.12021953616106361 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 1.389821227295533E-4 0.0 0.0 0.0 0.0 7 1.389821227295533E-4 0.0 0.0 0.0 0.0 8 1.389821227295533E-4 0.0 0.0 0.0 0.0 9 1.389821227295533E-4 0.0 0.0 0.0 0.0 10 1.389821227295533E-4 0.0 0.0 0.0 0.0 11 1.389821227295533E-4 0.0 0.0 0.0 0.0 12 1.389821227295533E-4 0.0 0.0 0.0 0.0 13 1.389821227295533E-4 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCCAACG 65 3.4223976E-6 14.592015 18 TAGGACC 310 0.0 12.58994 4 GAACAGT 200 0.0 12.3724 6 AGGCTTA 55 0.0030243478 12.112839 6 TGCCGGT 55 0.003101036 12.072414 10 CCAACGA 55 0.0031026513 12.071576 19 CTATAGT 120 9.678843E-9 11.899029 4 GGCGAGG 345 0.0 11.821647 19 TGTAGGA 775 0.0 11.799787 2 GGAGTGT 90 7.296103E-6 11.632171 6 GTAGGAC 770 0.0 11.50527 3 ATTATAC 100 1.862667E-6 11.431043 3 AGGACCT 570 0.0 11.353889 5 GTCCTAG 120 1.2097735E-7 11.122833 1 GGACCTG 540 0.0 11.1034355 6 CGGTTTC 60 0.005940795 11.065611 13 CTGTAGG 815 0.0 10.996105 1 GGACGTG 710 0.0 10.991731 6 AGGACGT 720 0.0 10.9712515 5 GATATAC 245 0.0 10.895837 1 >>END_MODULE