FastQCFastQC Report
Thu 26 May 2016
SRR1926908_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926908_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences719517
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT16370.22751373490827875No Hit
CTGTAGGACGTGGAATATGGCAAGA16140.22431714608549905No Hit
GTATCAACGCAGAGTACTTTTTTTT15320.21292061202167564No Hit
GTCCTACAGTGTGCATTTCTCATTT13240.18401233049392857No Hit
GTCCTAAAGTGTGTATTTCTCATTT12880.17900897407566466No Hit
CTTTAGGACGTGAAATATGGCGAGG12240.17011411822097322No Hit
CTGTAGGACCTGGAATATGGCGAGA10380.14426344339327632No Hit
GGTATCAACGCAGAGTACTTTTTTT9470.13161607022488697No Hit
TATCAACGCAGAGTACTTTTTTTTT7560.10507048478354229No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG250.005960708519.03755
TCCAACG653.401894E-614.6004718
ATTATAC400.005204466614.2791213
GGCGAGG3550.013.36662619
GCCTTAA500.001465254513.3392691
GGTCTAT500.001465254513.3392691
GACGTGG4500.013.076447
TAGGACC3600.012.6916684
TGTAGGA9900.012.6060632
GTAGGAC9700.012.5617013
AGGACGT8800.012.4392765
CTGTAGG9650.012.2432961
GGACGTG8600.012.1751476
GTACTAG550.002998720612.1266081
GTATCAC550.002998720612.1266081
CGTGGAA4850.011.9354319
GTGTAGG1052.6100133E-711.7966331
ACGTGGA5000.011.7671618
TCAACAC657.8780926E-411.7162033
TAGGACA2850.011.6896944
Produced by FastQC (version 0.11.5)