FastQCFastQC Report
Thu 26 May 2016
SRR1926907_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926907_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences567868
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT12700.22364352279050764No Hit
CTGTAGGACGTGGAATATGGCAAGA9910.1745123866814119No Hit
GTCCTAAAGTGTGTATTTCTCATTT8940.15743095226355422No Hit
GTCCTACAGTGTGCATTTCTCATTT8500.14968267273380434No Hit
CTTTAGGACGTGAAATATGGCGAGG7630.13436221093634437No Hit
GTACATGGGAAGCAGTGGTATCAAC7470.13154465474370805No Hit
GTATCAACGCAGAGTACTTTTTTTT7420.1306641684335092No Hit
CTGTAGGACCTGGAATATGGCGAGA6470.11393492853973106No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTTTC350.00219509316.2553713
GTATTGG400.00512764214.3104061
ATTAGGT400.0051669714.29394153
ATAATAC655.285594E-513.1944073
TAGGACC1900.013.0285574
GTCCTAT751.4118712E-512.7203621
GTCTAAT603.9416456E-412.7203621
TCCTATA603.980131E-412.7057272
TCCTACC550.002994434412.1281942
GACGTGA2450.012.0436187
GGCGAGG2450.011.99801119
TAGTCCA657.863485E-411.7179924
ACGTGAA2950.011.6063718
GTATTAT751.9896215E-411.4483261
TTAGGAC2750.011.4351543
CTATAGT752.027622E-411.4250424
TATAGGT752.027622E-411.4250424
TCCAACG752.105553E-411.37875818
GTCCTAC7300.011.3699121
GGACGTG4750.011.2216256
Produced by FastQC (version 0.11.5)