##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926907_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 567868 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.05092204526404 31.0 28.0 31.0 16.0 34.0 2 28.251343622109363 31.0 28.0 31.0 16.0 34.0 3 27.716768685680474 31.0 28.0 31.0 16.0 34.0 4 31.44691195841287 35.0 32.0 37.0 18.0 37.0 5 31.359317658329047 35.0 32.0 37.0 17.0 37.0 6 31.118073214197665 35.0 32.0 37.0 15.0 37.0 7 31.197896342107672 35.0 32.0 37.0 16.0 37.0 8 30.826190241394126 35.0 32.0 37.0 11.0 37.0 9 33.0215314122296 37.0 33.0 39.0 17.0 39.0 10 33.18548324610649 37.0 33.0 39.0 17.0 39.0 11 33.35055153662471 38.0 34.0 39.0 17.0 39.0 12 33.20929335690688 38.0 33.0 39.0 17.0 39.0 13 33.32418273260687 38.0 34.0 39.0 17.0 39.0 14 34.32807800404319 39.0 34.0 41.0 17.0 41.0 15 33.9003042960688 39.0 33.0 41.0 11.0 41.0 16 33.98024012622652 39.0 33.0 40.0 16.0 41.0 17 34.197366289348935 39.0 34.0 41.0 17.0 41.0 18 34.24605366035769 39.0 34.0 41.0 17.0 41.0 19 33.60537484063198 39.0 33.0 41.0 10.0 41.0 20 33.882617087069534 39.0 34.0 41.0 10.0 41.0 21 33.883210534842604 39.0 34.0 41.0 10.0 41.0 22 33.90774792733522 39.0 34.0 41.0 8.0 41.0 23 33.904992005184305 39.0 34.0 41.0 2.0 41.0 24 32.59543062824459 38.0 32.0 40.0 2.0 41.0 25 33.166256947036985 39.0 33.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.26621025011926847 1101 2 -0.22770637230336277 1101 3 -1.134285436343749 1101 4 -0.6560698178492217 1101 5 -0.665383188988315 1101 6 -2.4055048311333103 1101 7 -0.5429376103119452 1101 8 -0.1618169821337787 1101 9 -0.46811051447740937 1101 10 -9.717176702465906 1101 11 -4.247224224427125 1101 12 -1.4581167751288753 1101 13 -0.580621910541133 1101 14 -0.6589728558049472 1101 15 -2.2046716905766637 1101 16 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1103 2 0.22785877611697103 1103 3 0.3365253479080401 1103 4 0.031961796489536454 1103 5 -0.04335703556331083 1103 6 0.8886292490396457 1103 7 0.7275895994980459 1103 8 0.7418940527023601 1103 9 0.34710704467664044 1103 10 0.6379020175183712 1103 11 0.39509294375918813 1103 12 0.2525320066942811 1103 13 0.3938816587064835 1103 14 0.23048926524764113 1103 15 0.5947344876962006 1103 16 0.7198655279706614 1103 17 0.4794472253348516 1103 18 0.19600461055335217 1103 19 -1.0384206993730132 1103 20 -0.14279568061964198 1103 21 0.2289205943584065 1103 22 0.3592745413094036 1103 23 0.4344361159029404 1103 24 1.871825102263891 1103 25 1.1559621860538698 1104 1 -0.6698236968012559 1104 2 -0.6640352075176139 1104 3 -1.7357540602352657 1104 4 -0.8800730450980332 1104 5 -0.7826389555092739 1104 6 -1.8770188593689134 1104 7 -0.6019096582185348 1104 8 0.02054232293573932 1104 9 -0.12906038069045422 1104 10 0.014046224541566232 1104 11 -0.1565678714157528 1104 12 -0.2660813656646326 1104 13 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0.13546101287842305 2215 3 0.3995499798056592 2215 4 0.5499722297056948 2215 5 0.873224772610623 2215 6 1.1325288199517374 2215 7 0.4461404582993538 2215 8 -0.7625094150284788 2215 9 0.10295727673438648 2215 10 -0.24821454927798214 2215 11 -0.2709690800845834 2215 12 -0.32211427725432884 2215 13 -0.029396672979721927 2215 14 -0.06841487176024685 2215 15 -0.10249583117991534 2215 16 0.28470659030725187 2215 17 -0.3897259985789816 2215 18 -0.7607538367238789 2215 19 0.30921806615484115 2215 20 -3.9974437159474583 2215 21 -2.1147750659741043 2215 22 -0.988460550075061 2215 23 -0.8510787270358691 2215 24 -0.5994798003469164 2215 25 -0.3541132483507283 2216 1 -1.0435231617239644 2216 2 -1.6113449578148789 2216 3 -0.6908670209962651 2216 4 -0.7558162079993416 2216 5 -0.8593333669242575 2216 6 -2.309502509785087 2216 7 -10.257044913866572 2216 8 -11.37407717765879 2216 9 -6.27899349602934 2216 10 -3.9852106125571254 2216 11 -3.1009792863979193 2216 12 -2.557066799671766 2216 13 -2.5694587310099024 2216 14 -2.416183607635439 2216 15 -3.630130335809209 2216 16 -2.6240744842717376 2216 17 -2.9834213219004084 2216 18 -2.6441899925888634 2216 19 -1.876730483090622 2216 20 -2.8269162682548163 2216 21 -5.608644443388993 2216 22 -4.323311920637138 2216 23 -4.040417230352922 2216 24 -4.003718154214383 2216 25 -3.519514757427057 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 33730.0 3 9.0 4 271.0 5 1491.0 6 2013.0 7 1578.0 8 1218.0 9 1164.0 10 1119.0 11 1101.0 12 1075.0 13 1061.0 14 1031.0 15 1074.0 16 1179.0 17 1337.0 18 1452.0 19 1894.0 20 2396.0 21 3030.0 22 3832.0 23 4521.0 24 5211.0 25 6062.0 26 7103.0 27 7547.0 28 8594.0 29 10216.0 30 11921.0 31 14598.0 32 18819.0 33 24614.0 34 33192.0 35 45979.0 36 66082.0 37 104701.0 38 135653.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.28824710828106 18.519355191681168 12.687736711505812 25.504660988531956 2 14.216346594635768 19.541261434082557 42.60390292754829 23.638489043733383 3 19.129540914292363 24.59371346859467 29.077191355358117 27.199554261754848 4 12.17718614695729 16.44136881003611 38.27508366066284 33.10636138234377 5 12.772931917621943 37.889890157893625 35.74483375724824 13.592344167236192 6 32.50828308875984 36.79100547273934 17.998037775464443 12.702673663036377 7 28.10952391044797 30.970547301641126 22.567979992794015 18.351948795116886 8 24.802765086020383 37.41537928976003 20.444964085319224 17.33689153890036 9 26.18857223376898 15.621235072392292 19.994363617148693 38.19582907669003 10 14.814475524721916 29.28295345240067 34.515022003615 21.387549019262412 11 34.495609979480946 22.513892191311548 24.18042676107192 18.810071068135585 12 23.50525051342659 24.67635630924661 31.65983859206628 20.158554585260518 13 29.0448143947133 22.08963583460734 25.45406189895802 23.41148787172135 14 20.534069332844858 22.481919986473013 27.321339876073086 29.662670804609036 15 23.143702555612307 31.457984747344874 22.83829719780897 22.560015499233845 16 21.782054398331624 28.20244549422445 28.35075334044929 21.664746766994636 17 21.549311403454695 28.938316051635493 27.876755397191367 21.63561714771845 18 21.51955362363766 27.707953192478673 29.829227225012854 20.943265958870814 19 23.86712323458643 26.87215745956591 27.09885611417016 22.1618631916775 20 23.51702042592015 27.60220445646833 27.855304803622666 21.02547031398886 21 22.76954291833399 26.888420785661953 26.95006481429296 23.391971481711096 22 22.877015369115934 28.365230501512954 27.08863925377183 21.669114875599284 23 22.070357564944157 28.007580707943713 27.637528026955195 22.284533700156935 24 23.480904306658307 27.09221307144417 27.929873258984237 21.497009362913282 25 22.72326185548853 27.47056492448593 27.724362643652857 22.081810576372682 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 162.0 1 162.0 2 215.0 3 268.0 4 268.0 5 268.0 6 385.5 7 503.0 8 503.0 9 503.0 10 751.0 11 999.0 12 999.0 13 999.0 14 1671.5 15 2344.0 16 2344.0 17 2344.0 18 4190.0 19 6036.0 20 6036.0 21 6036.0 22 9691.0 23 13346.0 24 13346.0 25 13346.0 26 19443.0 27 25540.0 28 25540.0 29 25540.0 30 33989.5 31 42439.0 32 42439.0 33 42439.0 34 48415.5 35 54392.0 36 54392.0 37 54392.0 38 59068.0 39 63744.0 40 63744.0 41 63744.0 42 67355.5 43 70967.0 44 70967.0 45 70967.0 46 73772.5 47 76578.0 48 76578.0 49 76578.0 50 73248.5 51 69919.0 52 69919.0 53 69919.0 54 61986.0 55 54053.0 56 54053.0 57 54053.0 58 46530.5 59 39008.0 60 39008.0 61 39008.0 62 31637.0 63 24266.0 64 24266.0 65 24266.0 66 18778.5 67 13291.0 68 13291.0 69 13291.0 70 9734.0 71 6177.0 72 6177.0 73 6177.0 74 4307.0 75 2437.0 76 2437.0 77 2437.0 78 1685.5 79 934.0 80 934.0 81 934.0 82 631.0 83 328.0 84 328.0 85 328.0 86 209.0 87 90.0 88 90.0 89 90.0 90 63.0 91 36.0 92 36.0 93 36.0 94 23.0 95 10.0 96 10.0 97 10.0 98 5.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.22012157754971226 2 0.12397247247599795 3 0.1266139314065945 4 0.06991061302978861 5 0.02535800573372685 6 0.025534102995766618 7 0.2944346221304951 8 0.29320194129621674 9 0.022540449541090534 10 0.041206759317306135 11 0.01813801799009629 12 0.02007508787253376 13 0.018314115252136057 14 0.02007508787253376 15 0.01725753167989744 16 0.022892644065170074 17 0.020955574182732608 18 0.0169053371558179 19 0.02711897835412455 20 0.01919460156233491 21 0.016200948107658823 22 0.018666309776215598 23 0.01954679608641445 24 0.0169053371558179 25 0.01637704536969859 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 567868.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 85.84994572982008 #Duplication Level Percentage of deduplicated Percentage of total 1 93.72966867619611 80.46686969125453 2 4.7108527536524045 8.088529064844648 3 0.762456234813902 1.963704791404093 4 0.26262619032198337 0.9018577674548667 5 0.149853294130078 0.643244858425098 6 0.07943812002508693 0.4091854975819587 7 0.05645662013558315 0.33927584423102164 8 0.040918460269357 0.2810278074777678 9 0.026721881638363126 0.2064664879606935 >10 0.15207335442607522 2.417289724198358 >50 0.013543790315195014 0.7813213960689932 >100 0.013543749186817187 2.186989676769623 >500 0.0016416665680373472 1.0904999274144576 >1k 2.052083210046684E-4 0.22373746491379012 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 1270 0.22364352279050764 No Hit CTGTAGGACGTGGAATATGGCAAGA 991 0.1745123866814119 No Hit GTCCTAAAGTGTGTATTTCTCATTT 894 0.15743095226355422 No Hit GTCCTACAGTGTGCATTTCTCATTT 850 0.14968267273380434 No Hit CTTTAGGACGTGAAATATGGCGAGG 763 0.13436221093634437 No Hit GTACATGGGAAGCAGTGGTATCAAC 747 0.13154465474370805 No Hit GTATCAACGCAGAGTACTTTTTTTT 742 0.1306641684335092 No Hit CTGTAGGACCTGGAATATGGCGAGA 647 0.11393492853973106 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 1.760972620397698E-4 0.0 0.0 10 0.0 0.0 1.760972620397698E-4 0.0 0.0 11 0.0 0.0 1.760972620397698E-4 0.0 0.0 12 0.0 0.0 1.760972620397698E-4 0.0 0.0 13 0.0 0.0 1.760972620397698E-4 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGTTTC 35 0.002195093 16.25537 13 GTATTGG 40 0.005127642 14.310406 1 ATTAGGT 40 0.00516697 14.2939415 3 ATAATAC 65 5.285594E-5 13.194407 3 TAGGACC 190 0.0 13.028557 4 GTCCTAT 75 1.4118712E-5 12.720362 1 GTCTAAT 60 3.9416456E-4 12.720362 1 TCCTATA 60 3.980131E-4 12.705727 2 TCCTACC 55 0.0029944344 12.128194 2 GACGTGA 245 0.0 12.043618 7 GGCGAGG 245 0.0 11.998011 19 TAGTCCA 65 7.863485E-4 11.717992 4 ACGTGAA 295 0.0 11.606371 8 GTATTAT 75 1.9896215E-4 11.448326 1 TTAGGAC 275 0.0 11.435154 3 CTATAGT 75 2.027622E-4 11.425042 4 TATAGGT 75 2.027622E-4 11.425042 4 TCCAACG 75 2.105553E-4 11.378758 18 GTCCTAC 730 0.0 11.369912 1 GGACGTG 475 0.0 11.221625 6 >>END_MODULE