FastQCFastQC Report
Thu 26 May 2016
SRR1926907_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926907_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences567868
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT12430.21888889671543388No Hit
CTGTAGGACGTGGAATATGGCAAGA12090.21290158980608168No Hit
GTCCTACAGTGGACATTTCTAAATT11950.21043622813752494No Hit
CTTTAGGACGTGAAATATGGCGAGG9630.1695816633442983No Hit
GTCCTAAAGTGTGTATTTCTCATTT8910.1569026604774349No Hit
GTCCTACAGTGTGCATTTCTCATTT8750.15408510428479857No Hit
GGTATCAACGCAGAGTACTTTTTTT7760.13665147534286137No Hit
GTACATGGGAAGCAGTGGTATCAAC7510.13224904379186714No Hit
CTGTAGGACCTGGAATATGGCGAGA7290.1283749040269922No Hit
CCCATGTACTCTGCGTTGATACCAC7210.12696612593067402No Hit
TATCAACGCAGAGTACTTTTTTTTT6710.11816126282868554No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAGAC402.708804E-416.663663
CCTATAC551.1012713E-515.5816013
TAGGACC3450.014.34964
CCATATA400.005165627714.2945052
AGGCGTG400.00530668214.236597
GTATTAA551.9027462E-413.863791
GAGTAAG500.001460762113.3438981
GTAGGAC7350.012.9552263
TGTAGGA7550.012.874522
TAGGACA2150.012.8414814
CTGTAGG7250.012.75229551
TAGGCCT604.011603E-412.6938784
AGGACCT5750.012.4179255
GGACCTG5400.012.3401796
TTATACT853.837631E-612.3205284
GTATTAG550.002989594412.13081551
GTCCTAA2150.011.9696081
TTTATAC802.7916672E-511.9026143
TAAGACT657.8666926E-411.7174254
ACAGGTC658.08511E-411.6792488