##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926907_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 567868 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.742991328970817 31.0 30.0 33.0 25.0 34.0 2 30.101967358611507 31.0 30.0 34.0 26.0 34.0 3 30.190537237527032 31.0 30.0 34.0 26.0 34.0 4 33.795230581754915 35.0 35.0 37.0 28.0 37.0 5 33.76687716159389 37.0 35.0 37.0 28.0 37.0 6 33.712121478935245 37.0 35.0 37.0 29.0 37.0 7 33.703184542886724 37.0 35.0 37.0 29.0 37.0 8 33.73902914057492 37.0 35.0 37.0 29.0 37.0 9 35.280526460374595 39.0 35.0 39.0 29.0 39.0 10 35.1729486429945 39.0 35.0 39.0 29.0 39.0 11 35.208351236554975 39.0 35.0 39.0 29.0 39.0 12 35.09490585840371 39.0 35.0 39.0 29.0 39.0 13 35.120633668387725 39.0 35.0 39.0 29.0 39.0 14 36.19637662273627 40.0 36.0 41.0 29.0 41.0 15 36.188462811780205 40.0 36.0 41.0 29.0 41.0 16 36.15918488099347 40.0 36.0 41.0 29.0 41.0 17 36.13315066177351 40.0 36.0 41.0 29.0 41.0 18 36.113510534138214 40.0 36.0 41.0 29.0 41.0 19 36.17588242338008 40.0 37.0 41.0 29.0 41.0 20 36.14004839152761 40.0 36.0 41.0 28.0 41.0 21 36.086988525502406 40.0 36.0 41.0 28.0 41.0 22 35.99774419407327 40.0 36.0 41.0 28.0 41.0 23 35.934291067642484 40.0 36.0 41.0 28.0 41.0 24 35.86086203131714 40.0 36.0 41.0 27.0 41.0 25 35.740858791127515 40.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.08386611293603607 1101 2 -0.007195293166986971 1101 3 0.27845220400200077 1101 4 0.18249105417459788 1101 5 0.07837768274668377 1101 6 0.2120949447031606 1101 7 0.34336179588341054 1101 8 0.01605868002182831 1101 9 0.1588065773213998 1101 10 0.14697794346260906 1101 11 0.2888821524907641 1101 12 0.23585924688534732 1101 13 0.14068015992222627 1101 14 0.1483059326431757 1101 15 0.12019619451618269 1101 16 0.2350714714262523 1101 17 0.2140622880608447 1101 18 0.2995892736559824 1101 19 0.029687082160641864 1101 20 0.0919394784654699 1101 21 0.07353778044658554 1101 22 -0.023795030249210924 1101 23 -0.15912772332059433 1101 24 -0.06254390884494399 1101 25 -0.08131131037520589 1102 1 -0.5768310898344069 1102 2 -0.36493795144919616 1102 3 -0.17321597203766004 1102 4 -0.16866138399425523 1102 5 -0.39329500015318075 1102 6 -0.2768951359526284 1102 7 -0.36468720134018895 1102 8 -0.16237046660844356 1102 9 -0.31560423574169505 1102 10 -0.21009718929843757 1102 11 -0.37398344528471483 1102 12 -0.24423191073996264 1102 13 -0.2013735227352953 1102 14 -0.2026849388390275 1102 15 -0.29781934139626287 1102 16 -0.4046730831386398 1102 17 -0.14032573559591555 1102 18 -0.04619744981413021 1102 19 -0.12291265338019741 1102 20 -0.36151443463716504 1102 21 -0.24314993240689375 1102 22 -0.31190669834021634 1102 23 -0.22050605722791516 1102 24 -0.22053486961399926 1102 25 -0.38719956575261705 1103 1 -0.40922913017289275 1103 2 -0.2251713858761768 1103 3 -0.07300883609851283 1103 4 -0.3074834662406616 1103 5 -0.16983137619327948 1103 6 -0.2966684882321857 1103 7 -0.49583464960922896 1103 8 -0.276901795035549 1103 9 -0.19264042444041252 1103 10 -0.32723433025691406 1103 11 -0.32894092232655936 1103 12 -0.3862960592557272 1103 13 -0.5085115827269533 1103 14 -0.5827475744847916 1103 15 -0.41436830112140655 1103 16 -0.33634225947282914 1103 17 -0.2614316859822665 1103 18 -0.2174034334270587 1103 19 0.02326734987176593 1103 20 -0.455381655551534 1103 21 -0.4664749944416329 1103 22 -0.569298199404173 1103 23 -0.6615444233283156 1103 24 -0.7874991616403264 1103 25 -0.6272566871993135 1104 1 -0.5086838508843599 1104 2 -0.4041158658908124 1104 3 -0.11276708388749412 1104 4 -0.1932182564664373 1104 5 -0.2025537621947251 1104 6 -0.15662669521316275 1104 7 -0.2902636542458623 1104 8 -0.14380861911789822 1104 9 -0.1301676004565664 1104 10 -0.25408106807959996 1104 11 -0.23423259397696228 1104 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0.48629214667141696 1107 8 0.32711511113519975 1107 9 0.29873404724175145 1107 10 0.2962922969620152 1107 11 0.06949018502762527 1107 12 0.2843852594255907 1107 13 0.2628831763601198 1107 14 0.2844848853630566 1107 15 0.1461253590645626 1107 16 -0.016944119151617087 1107 17 -0.07699888801100485 1107 18 -0.04982946893002094 1107 19 0.20477876153702113 1107 20 0.17333178485421996 1107 21 0.031283247316608254 1107 22 0.20340171606497393 1107 23 0.15781652341724595 1107 24 0.05700464224809565 1107 25 0.12305269572545541 1108 1 0.06193671022371561 1108 2 0.08596860026380782 1108 3 -0.04379219342033025 1108 4 0.004967890441470502 1108 5 -0.052337106775929954 1108 6 -0.02086087588610752 1108 7 -0.051595884216617094 1108 8 -0.2214537434336563 1108 9 -0.4525516670439629 1108 10 -0.3158158111460878 1108 11 -0.42042101188357606 1108 12 -0.4175503905100584 1108 13 -0.3094977760208337 1108 14 -0.5022062343280638 1108 15 -0.5497446538054476 1108 16 -0.4991667703742664 1108 17 -0.34092552893764605 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0.31574408994127623 2215 18 0.20632275094313002 2215 19 0.02348126833255293 2215 20 0.11408805184304072 2215 21 0.2712059292858058 2215 22 0.4820885176354963 2215 23 0.33937254878722456 2215 24 0.4419590366908608 2215 25 0.2842511252029496 2216 1 -0.2402534109319241 2216 2 -0.04929273866132178 2216 3 -1.645949307430893 2216 4 -1.3920886488564506 2216 5 -0.733915236629926 2216 6 -0.3126537463427752 2216 7 -0.5188605626881895 2216 8 -0.38043168689435447 2216 9 -0.686387873940518 2216 10 -0.5730094271759114 2216 11 -0.7726169578295199 2216 12 -0.5019127701310566 2216 13 -0.5652449024576143 2216 14 -0.7218008289226603 2216 15 -0.8022687296053874 2216 16 -0.8499453506787091 2216 17 -0.7263227796366252 2216 18 -0.6459932620334641 2216 19 -0.764546361615686 2216 20 -0.6717137816482435 2216 21 -0.6378940925848653 2216 22 -0.8243150327035025 2216 23 -0.8439765800310326 2216 24 -0.9649744217913181 2216 25 -0.9943106047671577 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 13901.0 3 10.0 4 218.0 5 1440.0 6 1982.0 7 1386.0 8 1096.0 9 1005.0 10 941.0 11 847.0 12 738.0 13 737.0 14 691.0 15 761.0 16 691.0 17 779.0 18 847.0 19 1031.0 20 1298.0 21 1786.0 22 2300.0 23 2754.0 24 3224.0 25 3858.0 26 4516.0 27 5048.0 28 6004.0 29 7281.0 30 9148.0 31 11978.0 32 15486.0 33 20259.0 34 27533.0 35 38659.0 36 56746.0 37 97504.0 38 223385.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.76276553169808 19.302340751501646 10.692669923538155 28.242223793262117 2 14.171076801573898 20.525020537536975 40.159787326312376 25.14411533457675 3 18.607297089758852 24.425842039634514 29.260040719423447 27.706820151183187 4 12.253551881775342 17.158565018631087 37.40552381891567 33.1823592806779 5 13.066601859336394 38.4664965474247 35.1349066721612 13.331994921077703 6 32.09990847377367 37.5731061315504 17.502182140724223 12.824803253951705 7 28.53177398900944 31.545723545159927 21.18782584190503 18.7346766239256 8 25.147410021768074 38.404977774020615 19.326211157371205 17.121401046840106 9 26.22013934385809 15.775866320068193 19.604476192488956 38.39951814358476 10 15.010090906209165 29.692799706958674 33.560980420472035 21.736128966360123 11 34.57700008981451 22.68784880168076 24.048098309902315 18.687052798602416 12 23.70233013538453 24.96706958004029 31.115196596368143 20.215403688207033 13 29.246688479324344 22.146597965012873 25.330271578154 23.276441977508778 14 20.795714489484332 22.70390778122342 26.900665120529982 29.599712608762264 15 22.89953332746324 31.45355287487893 22.736285991018754 22.910627806639077 16 21.913345448910302 28.42810954735641 27.85807869489878 21.80046630883451 17 21.50446934851057 29.122436904351012 27.629308219515803 21.743785527622617 18 21.47662116281363 27.919184875251823 29.656572700505755 20.947621261428793 19 23.628420034972976 27.06936659464276 27.04436073939849 22.257852630985777 20 23.632595128411026 27.618760830057905 27.797853007058027 20.950791034473042 21 22.867352833431962 26.82232225885536 26.815453546369096 23.49487136134359 22 23.114702712602224 28.38723083533497 26.7437150887178 21.754351363345002 23 22.08333993699229 28.133524223101535 27.47263003484971 22.310505805056465 24 23.07807448209795 27.154373903794543 28.12854395739855 21.63900765670895 25 22.639658630825014 27.530374354341347 27.639737879756478 22.19022913507716 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 211.0 1 211.0 2 199.0 3 187.0 4 187.0 5 187.0 6 337.0 7 487.0 8 487.0 9 487.0 10 723.5 11 960.0 12 960.0 13 960.0 14 1555.5 15 2151.0 16 2151.0 17 2151.0 18 3832.0 19 5513.0 20 5513.0 21 5513.0 22 9102.0 23 12691.0 24 12691.0 25 12691.0 26 18634.5 27 24578.0 28 24578.0 29 24578.0 30 32924.5 31 41271.0 32 41271.0 33 41271.0 34 47249.5 35 53228.0 36 53228.0 37 53228.0 38 58282.5 39 63337.0 40 63337.0 41 63337.0 42 67248.5 43 71160.0 44 71160.0 45 71160.0 46 74303.5 47 77447.0 48 77447.0 49 77447.0 50 74373.5 51 71300.0 52 71300.0 53 71300.0 54 63228.0 55 55156.0 56 55156.0 57 55156.0 58 47324.0 59 39492.0 60 39492.0 61 39492.0 62 32325.5 63 25159.0 64 25159.0 65 25159.0 66 19368.5 67 13578.0 68 13578.0 69 13578.0 70 9909.0 71 6240.0 72 6240.0 73 6240.0 74 4361.0 75 2482.0 76 2482.0 77 2482.0 78 1684.0 79 886.0 80 886.0 81 886.0 82 573.0 83 260.0 84 260.0 85 260.0 86 167.0 87 74.0 88 74.0 89 74.0 90 45.0 91 16.0 92 16.0 93 16.0 94 10.0 95 4.0 96 4.0 97 4.0 98 2.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.11569590116012876 2 0.10812371889241867 3 0.014087780963181586 4 0.0 5 0.005459015123232864 6 0.3363457704959603 7 0.01901850430029514 8 0.011270224770545267 9 0.011270224770545267 10 0.005282917861193094 11 0.005459015123232864 12 7.043890481590792E-4 13 3.521945240795396E-4 14 1.760972620397698E-4 15 0.003169750716715856 16 7.043890481590792E-4 17 0.0 18 7.043890481590792E-4 19 1.760972620397698E-4 20 7.043890481590792E-4 21 0.013383391915022507 22 0.0 23 1.760972620397698E-4 24 0.0 25 0.0065155986954714825 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 567868.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 83.24660472535616 #Duplication Level Percentage of deduplicated Percentage of total 1 92.29855244386303 76.83541112016822 2 5.744181204537042 9.563671644098308 3 0.9845168234745676 2.4587304854775174 4 0.34636086917425696 1.1533346547392067 5 0.17832306834672826 0.742239499203638 6 0.1056853035914041 0.527876561601173 7 0.05562707327896165 0.32415354868975 8 0.04682326401754225 0.3118302201295466 9 0.03037528347043939 0.22757752948358811 >10 0.176080856216036 2.6992984131770132 >50 0.016313205123661297 0.9317416688167034 >100 0.014830152388213983 2.4562449723286734 >500 0.0016948745586268835 1.1246840086459164 >1k 6.355779594850812E-4 0.6432056734407492 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 1243 0.21888889671543388 No Hit CTGTAGGACGTGGAATATGGCAAGA 1209 0.21290158980608168 No Hit GTCCTACAGTGGACATTTCTAAATT 1195 0.21043622813752494 No Hit CTTTAGGACGTGAAATATGGCGAGG 963 0.1695816633442983 No Hit GTCCTAAAGTGTGTATTTCTCATTT 891 0.1569026604774349 No Hit GTCCTACAGTGTGCATTTCTCATTT 875 0.15408510428479857 No Hit GGTATCAACGCAGAGTACTTTTTTT 776 0.13665147534286137 No Hit GTACATGGGAAGCAGTGGTATCAAC 751 0.13224904379186714 No Hit CTGTAGGACCTGGAATATGGCGAGA 729 0.1283749040269922 No Hit CCCATGTACTCTGCGTTGATACCAC 721 0.12696612593067402 No Hit TATCAACGCAGAGTACTTTTTTTTT 671 0.11816126282868554 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTTAGAC 40 2.708804E-4 16.66366 3 CCTATAC 55 1.1012713E-5 15.581601 3 TAGGACC 345 0.0 14.3496 4 CCATATA 40 0.0051656277 14.294505 2 AGGCGTG 40 0.005306682 14.23659 7 GTATTAA 55 1.9027462E-4 13.86379 1 GAGTAAG 50 0.0014607621 13.343898 1 GTAGGAC 735 0.0 12.955226 3 TGTAGGA 755 0.0 12.87452 2 TAGGACA 215 0.0 12.841481 4 CTGTAGG 725 0.0 12.7522955 1 TAGGCCT 60 4.011603E-4 12.693878 4 AGGACCT 575 0.0 12.417925 5 GGACCTG 540 0.0 12.340179 6 TTATACT 85 3.837631E-6 12.320528 4 GTATTAG 55 0.0029895944 12.1308155 1 GTCCTAA 215 0.0 11.969608 1 TTTATAC 80 2.7916672E-5 11.902614 3 TAAGACT 65 7.8666926E-4 11.717425 4 ACAGGTC 65 8.08511E-4 11.679248 8 >>END_MODULE