FastQCFastQC Report
Thu 26 May 2016
SRR1926906_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926906_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences556083
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT8200.1474600014746No Hit
GTCCTACAGTGGACATTTCTAAATT7380.13271400132714No Hit
CTGTAGGACGTGGAATATGGCAAGA5740.10322200103222001No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG701.3369572E-917.61119318
AAAGGCG456.6514994E-414.8072115
GTATTAG400.00512677214.3106961
CCAACGA551.9866276E-413.79338219
CGGTTTC551.9881446E-413.7921413
GCCGGTT500.001519900613.27374111
GTCCTAC5000.012.9750311
GGACGTG3250.012.8871566
ACGTGGA1501.8189894E-1212.6792988
TGCGCCA604.153345E-412.64165812
GTGCGCC604.153345E-412.64165811
AGGACGT3500.012.5105835
GACGTGG1600.012.4924567
TAGGACC1700.012.324174
AGTACTG701.0684244E-412.2386135
GTAGGAC3750.012.2018523
TAGGACG3750.012.1897244
TGTATTA550.00298225212.1347192
GCGCCAC550.003107776212.06812313
TTAGGAC2500.011.8205443
Produced by FastQC (version 0.11.5)