##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926906_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 556083 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.003190171251415 31.0 28.0 31.0 16.0 34.0 2 28.202039983239914 31.0 28.0 31.0 16.0 34.0 3 27.652132865057915 31.0 27.0 31.0 16.0 34.0 4 31.402918269395037 35.0 32.0 37.0 18.0 37.0 5 31.30624385208683 35.0 32.0 37.0 17.0 37.0 6 31.077813923461065 35.0 32.0 37.0 15.0 37.0 7 31.172389733187313 35.0 32.0 37.0 16.0 37.0 8 30.7768336741098 35.0 31.0 37.0 11.0 37.0 9 33.010798747668964 37.0 33.0 39.0 17.0 39.0 10 33.19032410629349 37.0 33.0 39.0 17.0 39.0 11 33.313863218260586 37.0 33.0 39.0 17.0 39.0 12 33.2087368252581 38.0 33.0 39.0 17.0 39.0 13 33.31885707709101 38.0 34.0 39.0 17.0 39.0 14 34.32107617028393 39.0 34.0 41.0 17.0 41.0 15 33.893571643082055 39.0 33.0 40.0 11.0 41.0 16 33.97993824662865 39.0 33.0 40.0 17.0 41.0 17 34.19636277318314 39.0 34.0 41.0 17.0 41.0 18 34.2371984038354 39.0 34.0 41.0 17.0 41.0 19 33.61997579498025 39.0 33.0 40.0 10.0 41.0 20 33.902239413900446 39.0 34.0 41.0 10.0 41.0 21 33.892032304530076 39.0 34.0 41.0 10.0 41.0 22 33.91042704056768 39.0 34.0 41.0 8.0 41.0 23 33.89172119989282 39.0 34.0 41.0 2.0 41.0 24 32.590140320779454 38.0 32.0 40.0 2.0 41.0 25 33.1583360757297 39.0 33.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.3171923649698023 1101 2 -0.1958985314746542 1101 3 -1.245313413268839 1101 4 -0.4785900502002889 1101 5 -0.28034454567344724 1101 6 -2.135400236028257 1101 7 -0.24217965645985373 1101 8 -0.09774101109050903 1101 9 -0.1742531002385448 1101 10 -9.701465399763258 1101 11 -4.346518046596238 1101 12 -1.6237609798373285 1101 13 -0.7282710948493687 1101 14 -0.6035680232856464 1101 15 -2.0070810385041717 1101 16 -0.42870755356625523 1101 17 -0.15009087841777102 1101 18 -0.05935844633939524 1101 19 -0.8907361634598203 1101 20 -0.002247189743599165 1101 21 -0.029097476682807155 1101 22 0.15487857417764417 1101 23 0.11564251079908416 1101 24 0.05455503333819678 1101 25 -0.11283627771423 1102 1 -0.10588734864414207 1102 2 -0.2281309144452912 1102 3 -0.44709656702950085 1102 4 -0.1856020223516488 1102 5 -0.18101715138713814 1102 6 -0.644719107570932 1102 7 0.16514558410610292 1102 8 0.6155982344163355 1102 9 0.48727448638535265 1102 10 0.42726833936016817 1102 11 0.2002015608968648 1102 12 0.18883223767114998 1102 13 -0.06160682760376801 1102 14 -0.12747489503563258 1102 15 0.427313990663194 1102 16 0.24351809183002615 1102 17 0.09695495436369583 1102 18 -0.10522146051133774 1102 19 0.15920106768504638 1102 20 0.41152193604172993 1102 21 0.22805471763574303 1102 22 0.23861597001877755 1102 23 -0.23147357703124527 1102 24 -3.196076974171035 1102 25 -1.3362084624734933 1103 1 -0.13748492785715172 1103 2 -0.2124007644312691 1103 3 -0.0865342481211897 1103 4 -0.36260162511826266 1103 5 -0.6452164924470516 1103 6 0.07413312160936414 1103 7 0.16955020136005317 1103 8 0.20708604869804503 1103 9 0.08360212214570595 1103 10 0.23273643217257955 1103 11 -0.25280222450363965 1103 12 -0.45393665428136387 1103 13 -0.301303586646668 1103 14 -0.22731483334762004 1103 15 0.3079993935045806 1103 16 0.15421944242353902 1103 17 -0.2753870869485766 1103 18 -0.39177277371818064 1103 19 -1.734246918108017 1103 20 -0.3614285149349996 1103 21 -0.10403823497664888 1103 22 -0.2188055763874246 1103 23 -0.33245264049512 1103 24 1.1920755400826337 1103 25 0.5677369997192372 1104 1 -0.6736802337692644 1104 2 -0.7299816343834848 1104 3 -1.8472827490112884 1104 4 -1.0595325489447411 1104 5 -0.7312367070071559 1104 6 -2.185681363540077 1104 7 -0.9374839033902518 1104 8 -0.4332900756343854 1104 9 -0.4578181088109119 1104 10 -0.5882004529581266 1104 11 -0.6717975866323727 1104 12 -0.45569781562306844 1104 13 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5 -0.12663984932533623 2212 6 0.5048334403276016 2212 7 0.5985815563432553 2212 8 0.4899605964627085 2212 9 0.5446493161331318 2212 10 0.7749679443613999 2212 11 0.5046758097801032 2212 12 0.44479089546452144 2212 13 0.26215337562398133 2212 14 0.07064903089563757 2212 15 -0.9964613158588449 2212 16 0.13729022802091606 2212 17 0.17009163645568037 2212 18 0.11653435867909678 2212 19 0.9843334936414863 2212 20 -0.2817260750176942 2212 21 -0.15617989231147789 2212 22 0.28089158745097365 2212 23 0.4205084241960648 2212 24 1.2769854160409437 2212 25 0.8971850572607991 2213 1 -0.5622615401337647 2213 2 -0.6101051048439459 2213 3 0.24964539014177944 2213 4 -7.193574279007581 2213 5 -1.6537122405261329 2213 6 -3.4568174093411663 2213 7 -1.4344060275961752 2213 8 -3.3623273696737073 2213 9 -1.234033367364784 2213 10 -0.2979888225110088 2213 11 -0.35649405736214135 2213 12 -0.2876976078339837 2213 13 -0.4031810687324864 2213 14 -0.5120046805374656 2213 15 -2.5061368945900426 2213 16 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0.03860905862903863 2215 3 0.3811669896259424 2215 4 0.7972203968122926 2215 5 0.8860542751223122 2215 6 1.2323820001606194 2215 7 0.7459540890490395 2215 8 -0.10948699084340774 2215 9 0.23612174749875692 2215 10 0.00398944347013952 2215 11 0.2433246238100324 2215 12 -0.29421587425146356 2215 13 -0.1639650544984761 2215 14 -0.17677695330110055 2215 15 -0.24268645547365963 2215 16 0.0401899109399011 2215 17 -0.3662348191299998 2215 18 -1.0060255565724745 2215 19 0.013302508707070615 2215 20 -3.406424049907585 2215 21 -1.9543352112185488 2215 22 -1.1598957699411727 2215 23 -0.9224385185479562 2215 24 -1.128508236292177 2215 25 -0.998854211445412 2216 1 -0.770357786439618 2216 2 -1.3559649649687238 2216 3 -0.4425029246411789 2216 4 -0.6096099388569236 2216 5 -0.45890266980681105 2216 6 -2.1233811545075305 2216 7 -10.430340628334271 2216 8 -11.62361075177223 2216 9 -6.427761037074983 2216 10 -3.996809881230174 2216 11 -2.8332313477821067 2216 12 -2.6562533741335663 2216 13 -2.169779611168 2216 14 -2.2643277658299894 2216 15 -3.5473447029011993 2216 16 -2.626274763152548 2216 17 -2.908301934110149 2216 18 -2.614585168746732 2216 19 -1.720642618477516 2216 20 -2.6725166489625423 2216 21 -5.3717784096491386 2216 22 -4.089192081258101 2216 23 -3.8364415555102376 2216 24 -4.040971571103121 2216 25 -3.3501963224171405 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 33318.0 3 9.0 4 245.0 5 1388.0 6 1796.0 7 1526.0 8 1206.0 9 1166.0 10 1117.0 11 1087.0 12 1011.0 13 1022.0 14 1056.0 15 1037.0 16 1105.0 17 1212.0 18 1459.0 19 1825.0 20 2317.0 21 2975.0 22 3807.0 23 4481.0 24 5120.0 25 6039.0 26 6821.0 27 7452.0 28 8161.0 29 9918.0 30 11848.0 31 14404.0 32 18470.0 33 24152.0 34 33017.0 35 46046.0 36 65911.0 37 102963.0 38 129596.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.72693460961827 18.233551493484025 12.571358551493484 25.46815534540422 2 14.367301816505734 19.558089833221402 42.40457898442043 23.67002936585243 3 19.48181490817429 24.130176449405834 28.944184371624054 27.443824270795826 4 12.088127638592463 16.326611408437017 38.3272088595522 33.25805209341832 5 12.92805416353387 37.65545574570192 35.79430376331514 13.622186327449068 6 32.78651370106954 36.84287209816851 17.804735026315697 12.565879174446254 7 28.234833645372266 31.04643067144285 22.11778838349404 18.600947299690848 8 24.758720075607958 37.411148927113715 20.238294925140547 17.59183607213778 9 25.914361053453295 15.912238606235679 19.77185491094715 38.40154542936388 10 14.356311851576006 29.695592650759984 34.5683409880421 21.379754509621907 11 34.81074586489967 22.842157518723965 23.49812580489665 18.848970811479717 12 22.684341640211166 25.113631253765973 31.65792810698515 20.54409899903771 13 28.810800407929076 21.958023594416336 25.506359053129707 23.724816944524882 14 20.44624709966365 22.185009982553016 27.5727107577747 29.796032160008636 15 23.384833986834057 30.659556099140257 23.01431706176481 22.941292852260872 16 22.038074955572025 28.661620703796704 27.471958212520413 21.828346128110855 17 21.64514545032673 28.728890231903918 27.56766187677957 22.058302440989777 18 21.35902947021952 28.426126604135003 28.947003965934332 21.267839959711143 19 23.54191849421516 27.279938697979624 27.031888562712254 22.146254245092962 20 22.888146641269564 27.798312495840594 28.228012712939034 21.085528149950807 21 22.926765219549658 27.091670728570683 27.145090012392554 22.836474039487104 22 22.42937029982211 28.041074675832746 27.58564868525423 21.94390633909091 23 22.46298348106366 27.616157764475652 27.499244560838033 22.421614193622656 24 22.986465218759836 27.321372363865283 27.844057736409013 21.84810468096587 25 22.673379162484846 27.33057782861912 27.897866462342037 22.098176546554 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 135.0 1 135.0 2 202.5 3 270.0 4 270.0 5 270.0 6 413.0 7 556.0 8 556.0 9 556.0 10 849.0 11 1142.0 12 1142.0 13 1142.0 14 1845.0 15 2548.0 16 2548.0 17 2548.0 18 4339.5 19 6131.0 20 6131.0 21 6131.0 22 9622.0 23 13113.0 24 13113.0 25 13113.0 26 18839.0 27 24565.0 28 24565.0 29 24565.0 30 32305.5 31 40046.0 32 40046.0 33 40046.0 34 45886.5 35 51727.0 36 51727.0 37 51727.0 38 56998.0 39 62269.0 40 62269.0 41 62269.0 42 66038.5 43 69808.0 44 69808.0 45 69808.0 46 72070.0 47 74332.0 48 74332.0 49 74332.0 50 71906.5 51 69481.0 52 69481.0 53 69481.0 54 61931.0 55 54381.0 56 54381.0 57 54381.0 58 46634.0 59 38887.0 60 38887.0 61 38887.0 62 31553.0 63 24219.0 64 24219.0 65 24219.0 66 18594.0 67 12969.0 68 12969.0 69 12969.0 70 9425.0 71 5881.0 72 5881.0 73 5881.0 74 4120.0 75 2359.0 76 2359.0 77 2359.0 78 1610.0 79 861.0 80 861.0 81 861.0 82 575.0 83 289.0 84 289.0 85 289.0 86 187.5 87 86.0 88 86.0 89 86.0 90 54.0 91 22.0 92 22.0 93 22.0 94 13.0 95 4.0 96 4.0 97 4.0 98 3.0 99 2.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.20482553863362124 2 0.12156458658149952 3 0.12282339147213636 4 0.06995358606538953 5 0.02463661000246366 6 0.025355927082827565 7 0.2994157347014744 8 0.29563932002956395 9 0.02175934168100805 10 0.03884312233965074 11 0.019961048980098293 12 0.021219853870735123 13 0.018702244089461462 14 0.020320707520280246 15 0.01852241481937049 16 0.022838317301553904 17 0.021579512410917077 18 0.017623268468915612 19 0.026434902703373418 20 0.02050053679037122 21 0.018702244089461462 22 0.021579512410917077 23 0.020680366060462196 24 0.01942156116982537 25 0.018882073359552443 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 556083.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 88.78073125464762 #Duplication Level Percentage of deduplicated Percentage of total 1 94.70151241079648 84.07669522751598 2 4.036812894092081 7.167824013513706 3 0.6211485548088276 1.6543806874108586 4 0.22201717494043666 0.7884338856921198 5 0.11679053943310022 0.5184374747247698 6 0.07033003787286077 0.37463713149117844 7 0.04032600809768882 0.2506120741245559 8 0.03074967034317066 0.21839825751248337 9 0.025919100643977573 0.2071005037771623 >10 0.11535151299062182 1.9308395795531996 >50 0.008913330878044894 0.5798302993390928 >100 0.008913313290494537 1.5635970731730615 >500 0.0012154518122861517 0.669213792171717 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 820 0.1474600014746 No Hit GTCCTACAGTGGACATTTCTAAATT 738 0.13271400132714 No Hit CTGTAGGACGTGGAATATGGCAAGA 574 0.10322200103222001 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCCAACG 70 1.3369572E-9 17.611193 18 AAAGGCG 45 6.6514994E-4 14.807211 5 GTATTAG 40 0.005126772 14.310696 1 CCAACGA 55 1.9866276E-4 13.793382 19 CGGTTTC 55 1.9881446E-4 13.79214 13 GCCGGTT 50 0.0015199006 13.273741 11 GTCCTAC 500 0.0 12.975031 1 GGACGTG 325 0.0 12.887156 6 ACGTGGA 150 1.8189894E-12 12.679298 8 TGCGCCA 60 4.153345E-4 12.641658 12 GTGCGCC 60 4.153345E-4 12.641658 11 AGGACGT 350 0.0 12.510583 5 GACGTGG 160 0.0 12.492456 7 TAGGACC 170 0.0 12.32417 4 AGTACTG 70 1.0684244E-4 12.238613 5 GTAGGAC 375 0.0 12.201852 3 TAGGACG 375 0.0 12.189724 4 TGTATTA 55 0.002982252 12.134719 2 GCGCCAC 55 0.0031077762 12.068123 13 TTAGGAC 250 0.0 11.820544 3 >>END_MODULE