FastQCFastQC Report
Thu 26 May 2016
SRR1926906_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926906_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences556083
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT12390.2228084656427188No Hit
GGTATCAACGCAGAGTACTTTTTTT9060.1629253187024239No Hit
GTCCTACAGTGGACATTTCTAAATT7790.14008700140087No Hit
CTGTAGGACGTGGAATATGGCAAGA7200.12947707446550244No Hit
TATCAACGCAGAGTACTTTTTTTTT6440.11581004993858832No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGTAT551.8951208E-413.8702531
TCCAACG707.3463398E-613.55273418
GTCTTAG655.2514748E-513.2034131
CCTGTAC957.074959E-813.038383
TAGGACC2050.013.0127414
GACGTGG2300.012.7901617
CGTGGAA2250.012.6469439
GGACGTG3550.012.3450856
ACTAGGA701.05178406E-412.2592042
GTATACC550.002997244212.1266043
TTAAAGC550.002999273412.1255094
CCTGGAC1052.605866E-711.7966293
CTGTACC1052.60894E-711.7955634
CTAGGAC907.1961276E-611.64539053
TGTAGAA2300.011.6077482
CTGTAGG5150.011.479991
TGTAGGA5650.011.4756762
GTATAAG751.9981718E-411.4429581
GTAGGAC5250.011.4336563
TAGGACG4150.011.2489674
Produced by FastQC (version 0.11.5)