##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926906_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 556083 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.81937048965712 31.0 30.0 33.0 26.0 34.0 2 30.102376803462793 31.0 30.0 34.0 26.0 34.0 3 30.15427553081105 31.0 30.0 34.0 26.0 34.0 4 33.75199205873943 35.0 35.0 37.0 28.0 37.0 5 33.805529030738214 37.0 35.0 37.0 30.0 37.0 6 33.76783861401985 37.0 35.0 37.0 29.0 37.0 7 33.76883486817616 37.0 35.0 37.0 29.0 37.0 8 33.807323726853724 37.0 35.0 37.0 29.0 37.0 9 35.32589199813697 39.0 35.0 39.0 30.0 39.0 10 35.21830374242694 39.0 35.0 39.0 29.0 39.0 11 35.26114806602612 39.0 35.0 39.0 30.0 39.0 12 35.15657195059011 39.0 35.0 39.0 29.0 39.0 13 35.167974924606575 39.0 35.0 39.0 29.0 39.0 14 36.26570314143752 40.0 36.0 41.0 29.0 41.0 15 36.2524155566705 40.0 36.0 41.0 29.0 41.0 16 36.2304817805975 40.0 36.0 41.0 29.0 41.0 17 36.20371059715906 40.0 36.0 41.0 29.0 41.0 18 36.16855217656357 40.0 36.0 41.0 29.0 41.0 19 36.247313800278015 40.0 37.0 41.0 29.0 41.0 20 36.18881354042472 40.0 37.0 41.0 29.0 41.0 21 36.151506160051646 40.0 37.0 41.0 29.0 41.0 22 36.0565365242239 40.0 36.0 41.0 28.0 41.0 23 35.989393669650035 40.0 36.0 41.0 28.0 41.0 24 35.906001442230746 40.0 36.0 41.0 27.0 41.0 25 35.79710223114176 40.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.07338453959507163 1101 2 0.17071142965941277 1101 3 0.2704702206279741 1101 4 0.34073112488224666 1101 5 -0.027212565560333246 1101 6 0.10665803410593355 1101 7 0.05340734469163522 1101 8 0.0421359724282766 1101 9 0.28907212114398817 1101 10 0.19382710221022847 1101 11 0.12075785264786276 1101 12 0.31750002032803337 1101 13 0.18034263959978603 1101 14 0.11623519103890345 1101 15 0.012425878617754904 1101 16 0.16713041667377837 1101 17 0.1907959915191242 1101 18 0.07678648322846726 1101 19 0.2508550010880697 1101 20 0.22931988230780576 1101 21 0.28525082940343793 1101 22 0.010045200850370861 1101 23 0.18180487049617255 1101 24 0.24921286679458632 1101 25 0.43673915339115155 1102 1 -0.6086796919471418 1102 2 -0.4593021606615153 1102 3 -0.28058130166072814 1102 4 -0.4831259348863668 1102 5 -0.2927694519947792 1102 6 -0.5271965494601432 1102 7 -0.25355827064469594 1102 8 -0.3892861740362221 1102 9 -0.05480618789761138 1102 10 -0.07132092287427838 1102 11 -0.2662538891590742 1102 12 -0.19765520301967854 1102 13 -0.2445175701288136 1102 14 -0.14344215401128224 1102 15 -0.20215578711793114 1102 16 -0.3278466352631355 1102 17 -0.12765805188928425 1102 18 -0.197696249577767 1102 19 -0.16937384609568085 1102 20 -0.08081584814315335 1102 21 -0.02707317306124679 1102 22 0.06538256905016482 1102 23 0.06036585130272698 1102 24 -0.1197312343726864 1102 25 -0.061043781542309716 1103 1 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-0.5504398031269844 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 13215.0 3 5.0 4 192.0 5 1315.0 6 1921.0 7 1447.0 8 1090.0 9 925.0 10 886.0 11 813.0 12 702.0 13 702.0 14 682.0 15 650.0 16 693.0 17 739.0 18 919.0 19 1034.0 20 1341.0 21 1702.0 22 2227.0 23 2609.0 24 3116.0 25 3724.0 26 4534.0 27 5063.0 28 5784.0 29 6888.0 30 8979.0 31 11248.0 32 14768.0 33 20059.0 34 27247.0 35 37721.0 36 55392.0 37 95825.0 38 219926.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.127578365941176 19.04115483428423 10.560475587778315 28.270791211996276 2 14.410181634894062 20.435995751651635 40.075246170185956 25.078576443268346 3 19.28978289529298 23.801661513779703 28.82451227430265 28.08404331662467 4 12.210407439177246 17.097447683169598 37.38075071527092 33.311394162382236 5 13.069546952105544 38.359805487217116 35.24644999784194 13.3241975628354 6 32.34258763074469 37.71437438315209 17.214725321121772 12.728312664981441 7 28.79313042868269 31.466349137256522 20.770596392899478 18.96992404116131 8 25.2606419426255 38.45648893676791 19.046779765876362 17.236089354730222 9 25.883715534869335 16.029444295572386 19.51071249368293 38.576127675875355 10 14.522223121397678 30.144677330887575 33.7305890498413 21.60251049787345 11 34.96973337313734 22.998320319103403 23.181754421301378 18.850191886457885 12 22.845274061286148 25.23701625665372 31.351963746223564 20.56574593583657 13 28.930421756620913 21.822798559197523 25.49314036314984 23.753639321031724 14 20.657707572430734 22.38029215063219 27.13084197862549 29.831158298311582 15 22.917232387717483 30.888639122420535 23.03574158162118 23.158386908240793 16 22.143356813126143 28.879425839041435 27.10756166817424 21.86965567965818 17 21.640834192018097 28.887953776684416 27.44050798172215 22.030704049575334 18 21.47446675742116 28.377262942855243 28.970164311186004 21.1781059885376 19 23.242034012908146 27.29268832170737 27.214642418487887 22.250635246896596 20 22.94278330956573 27.932002467275336 27.970126546767638 21.155087676391304 21 22.913785320285818 27.008609418023482 27.107167201120824 22.970438060569872 22 22.436578712170665 28.127275964199587 27.320561858571473 22.115583465058275 23 22.299629010881507 27.68391655172538 27.48988007142844 22.52657436596467 24 22.634750567810922 27.30851329747538 28.286604697500195 21.77013143721351 25 22.652401685469034 27.425379509286085 27.670862355160626 22.251356450084256 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 204.0 1 204.0 2 195.0 3 186.0 4 186.0 5 186.0 6 333.0 7 480.0 8 480.0 9 480.0 10 764.0 11 1048.0 12 1048.0 13 1048.0 14 1699.0 15 2350.0 16 2350.0 17 2350.0 18 4035.5 19 5721.0 20 5721.0 21 5721.0 22 9108.0 23 12495.0 24 12495.0 25 12495.0 26 17962.0 27 23429.0 28 23429.0 29 23429.0 30 31535.5 31 39642.0 32 39642.0 33 39642.0 34 45338.0 35 51034.0 36 51034.0 37 51034.0 38 55903.0 39 60772.0 40 60772.0 41 60772.0 42 65319.0 43 69866.0 44 69866.0 45 69866.0 46 72633.0 47 75400.0 48 75400.0 49 75400.0 50 73304.0 51 71208.0 52 71208.0 53 71208.0 54 63328.0 55 55448.0 56 55448.0 57 55448.0 58 47651.5 59 39855.0 60 39855.0 61 39855.0 62 32061.5 63 24268.0 64 24268.0 65 24268.0 66 18747.0 67 13226.0 68 13226.0 69 13226.0 70 9602.5 71 5979.0 72 5979.0 73 5979.0 74 4140.0 75 2301.0 76 2301.0 77 2301.0 78 1543.5 79 786.0 80 786.0 81 786.0 82 539.0 83 292.0 84 292.0 85 292.0 86 179.0 87 66.0 88 66.0 89 66.0 90 45.0 91 24.0 92 24.0 93 24.0 94 13.5 95 3.0 96 3.0 97 3.0 98 1.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.11581004993858832 2 0.10304217176212904 3 0.015105658687641953 4 0.0 5 0.004855390292456342 6 0.3334034667486688 7 0.022298829491280977 8 0.009351122044730731 9 0.008991463504548781 10 0.003236926861637561 11 0.0044957317522743906 12 5.394878102729268E-4 13 7.193170803639025E-4 14 0.0 15 0.001438634160727805 16 3.5965854018195126E-4 17 0.0 18 7.193170803639025E-4 19 0.0 20 7.193170803639025E-4 21 0.01150907328582244 22 0.0 23 3.5965854018195126E-4 24 0.0 25 0.006473853723275122 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 556083.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 86.35625153428298 #Duplication Level Percentage of deduplicated Percentage of total 1 93.30782763140385 80.57714233055032 2 5.094029153966668 8.798025258858326 3 0.790305906389603 2.0474356692363016 4 0.29831530379546695 1.0304556564434955 5 0.14156121123360874 0.6112347782393642 6 0.0801499366296166 0.4152868852826408 7 0.058621460492811424 0.3543630711336979 8 0.04034299609624477 0.2787095934827128 9 0.028003029046908705 0.21764129580870126 >10 0.13914731792119814 2.216718527405312 >50 0.00980496189614961 0.6330576364736763 >100 0.010430781691131475 1.8575538863096617 >500 0.0012516938028964554 0.739166287984436 >1k 2.086156338160759E-4 0.22320912279130298 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 1239 0.2228084656427188 No Hit GGTATCAACGCAGAGTACTTTTTTT 906 0.1629253187024239 No Hit GTCCTACAGTGGACATTTCTAAATT 779 0.14008700140087 No Hit CTGTAGGACGTGGAATATGGCAAGA 720 0.12947707446550244 No Hit TATCAACGCAGAGTACTTTTTTTTT 644 0.11581004993858832 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCTGTAT 55 1.8951208E-4 13.870253 1 TCCAACG 70 7.3463398E-6 13.552734 18 GTCTTAG 65 5.2514748E-5 13.203413 1 CCTGTAC 95 7.074959E-8 13.03838 3 TAGGACC 205 0.0 13.012741 4 GACGTGG 230 0.0 12.790161 7 CGTGGAA 225 0.0 12.646943 9 GGACGTG 355 0.0 12.345085 6 ACTAGGA 70 1.05178406E-4 12.259204 2 GTATACC 55 0.0029972442 12.126604 3 TTAAAGC 55 0.0029992734 12.125509 4 CCTGGAC 105 2.605866E-7 11.796629 3 CTGTACC 105 2.60894E-7 11.795563 4 CTAGGAC 90 7.1961276E-6 11.6453905 3 TGTAGAA 230 0.0 11.607748 2 CTGTAGG 515 0.0 11.47999 1 TGTAGGA 565 0.0 11.475676 2 GTATAAG 75 1.9981718E-4 11.442958 1 GTAGGAC 525 0.0 11.433656 3 TAGGACG 415 0.0 11.248967 4 >>END_MODULE