FastQCFastQC Report
Thu 26 May 2016
SRR1926905_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926905_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences569471
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT8020.14083245678884437No Hit
GTCCTACAGTGGACATTTCTAAATT7550.1325791831366303No Hit
GTACATGGGAAGCAGTGGTATCAAC6430.11291180762497124No Hit
CTGTAGGACGTGGAATATGGCAAGA5920.10395612770448363No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTTAAT359.593036E-519.084511
GCGTCGT307.550128E-419.0609234
AGCGTCG350.002120790516.3393753
CGTCGTC350.002133335516.324965
TGGTCTA350.00219313716.2575510
ATCTAGT456.529457E-414.843511
TAGCGTC400.00515977314.2969532
TAGGACG3000.014.29569154
AGGACGT2950.013.8808285
TGTAGAA2100.013.6161462
GGACGTG2800.013.6041346
GGCGAGG1351.8189894E-1213.34255919
ACGTGAA1650.013.2626368
GACGTGA1550.012.9019847
TTAGGAC1850.012.8801383
GTCTAGA1051.8641003E-812.7230081
AATCCCG1207.6033757E-1012.64031919
GACGTGG1501.2732926E-1112.0623327
TGTAGGA4050.012.002382
CTGTAGG3900.011.7443151
Produced by FastQC (version 0.11.5)