##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926905_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 569471 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.965936808020075 31.0 28.0 31.0 16.0 34.0 2 28.16328662916988 31.0 28.0 31.0 16.0 34.0 3 27.627752774065755 31.0 28.0 31.0 16.0 34.0 4 31.351234742418843 35.0 32.0 37.0 17.0 37.0 5 31.26743767461381 35.0 32.0 37.0 17.0 37.0 6 31.02593459544033 35.0 32.0 37.0 12.0 37.0 7 31.12541288318457 35.0 32.0 37.0 13.0 37.0 8 30.730182924152416 35.0 31.0 37.0 11.0 37.0 9 32.938356825896314 37.0 33.0 39.0 17.0 39.0 10 33.11140865821086 37.0 33.0 39.0 17.0 39.0 11 33.25521756156152 38.0 33.0 39.0 17.0 39.0 12 33.13164849483117 38.0 33.0 39.0 17.0 39.0 13 33.23763984469797 38.0 34.0 39.0 17.0 39.0 14 34.243903552595306 39.0 34.0 41.0 17.0 41.0 15 33.81198164612421 39.0 33.0 41.0 10.0 41.0 16 33.88603985101963 39.0 33.0 40.0 11.0 41.0 17 34.103571560272606 39.0 34.0 41.0 17.0 41.0 18 34.15397272205257 39.0 34.0 41.0 16.0 41.0 19 33.54068776109758 39.0 33.0 40.0 10.0 41.0 20 33.816598211322436 39.0 34.0 41.0 10.0 41.0 21 33.799262824621444 39.0 34.0 41.0 8.0 41.0 22 33.82404898581315 39.0 34.0 41.0 2.0 41.0 23 33.81983454820351 39.0 34.0 41.0 2.0 41.0 24 32.53079963685596 38.0 32.0 40.0 2.0 41.0 25 33.09270533530241 39.0 33.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.8626722574174011 1101 2 -0.9865351982439456 1101 3 -1.8930390300075963 1101 4 -1.3297509120124182 1101 5 -1.3432390447946716 1101 6 -2.9862887664270197 1101 7 -1.5514425474390237 1101 8 -0.8386465090658923 1101 9 -0.6771483519322601 1101 10 -10.048863678218922 1101 11 -4.553995856323308 1101 12 -2.226539315741036 1101 13 -1.6118309782536073 1101 14 -1.386555953999256 1101 15 -3.0371220520158673 1101 16 -1.3521303049731301 1101 17 -1.2732403445493645 1101 18 -1.220296135953184 1101 19 -1.654834880220605 1101 20 -0.7774353878147124 1101 21 -1.12003216087043 1101 22 -0.8040456389291393 1101 23 -0.8608212200639471 1101 24 -1.0186297461680134 1101 25 -0.8735156115997142 1102 1 -0.38423454785250755 1102 2 -0.6148297697031069 1102 3 -0.9081826471187959 1102 4 -0.7815136521491297 1102 5 -0.42189033444646284 1102 6 -0.8421884232336865 1102 7 -0.05337265772298849 1102 8 0.27045966672348243 1102 9 0.3656099306358911 1102 10 0.1048379070074219 1102 11 -0.06861256741538568 1102 12 0.04204891424921442 1102 13 -0.21588928167258103 1102 14 -0.3024623168825329 1102 15 0.19892198267540095 1102 16 0.10539318038339474 1102 17 -0.10369072240014532 1102 18 -0.22386754439833112 1102 19 0.20811883658009123 1102 20 0.14091672065467264 1102 21 -0.08520564538164876 1102 22 -0.13546388960115507 1102 23 -0.5896984875913205 1102 24 -3.2675562103750337 1102 25 -1.4290703844665877 1103 1 -0.5842334140640517 1103 2 -0.6802096809558158 1103 3 -0.4403886167984581 1103 4 -0.8111423789472134 1103 5 -0.9943032772085694 1103 6 -0.13490142311038866 1103 7 -0.07691577498931323 1103 8 -0.22887635841260945 1103 9 -0.5143360443442333 1103 10 -0.18660099809306274 1103 11 -0.2158803498243742 1103 12 -0.5283217920584775 1103 13 -0.33017232345693515 1103 14 -0.6338289996584479 1103 15 -0.17277355544185724 1103 16 -0.02202054894445382 1103 17 -0.27762804373988814 1103 18 -0.36213871541756504 1103 19 -2.266785464588139 1103 20 -0.9960803555883544 1103 21 -0.8365814268099143 1103 22 -0.6795377899326454 1103 23 -0.6247977436843684 1103 24 0.9453210754487813 1103 25 0.04698187751224481 1104 1 -0.30315905577561963 1104 2 -0.3254423105901658 1104 3 -1.664229600343024 1104 4 -0.8552004806826758 1104 5 -0.43449633340411253 1104 6 -1.899848782243371 1104 7 -0.39912662137490074 1104 8 0.2865755603144322 1104 9 0.031148549956235172 1104 10 0.10552675177422799 1104 11 0.12385317548204 1104 12 0.06520415862144091 1104 13 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24 -4.873065985559617 1105 25 -2.3190407962134003 1106 1 0.11528982789065623 1106 2 -0.45347424057112207 1106 3 -0.05122664236255403 1106 4 -0.580998392537559 1106 5 -0.025695328501157633 1106 6 0.3739035307893026 1106 7 -2.664391286339715 1106 8 -0.15807778868674305 1106 9 -0.3683179330184885 1106 10 -0.06527703552131214 1106 11 0.049302612925480105 1106 12 -0.0989340425482439 1106 13 -0.30059003474431734 1106 14 -0.49413889238788755 1106 15 0.3872691883828949 1106 16 0.2439583805419332 1106 17 -0.21054763876954752 1106 18 -0.4892262443586972 1106 19 0.32817432607672004 1106 20 0.1502706643724636 1106 21 -0.36122625576611256 1106 22 -0.5424397120347635 1106 23 -0.6701582577062624 1106 24 -3.532796004271191 1106 25 -1.726097732861355 1107 1 0.23526738120154178 1107 2 0.23965242841761736 1107 3 0.6350499163277981 1107 4 0.22798415322659693 1107 5 0.4741382563898995 1107 6 0.406582814043297 1107 7 -1.7853402394725748 1107 8 -1.6301316607406164 1107 9 -0.14183470998354153 1107 10 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1110 8 1.2619444582543018 1110 9 0.947329082183586 1110 10 0.9235253374857351 1110 11 0.5722911186726023 1110 12 0.6924267286790453 1110 13 0.6880269470873444 1110 14 0.6324462021616171 1110 15 1.2308731928322771 1110 16 1.1655749915912494 1110 17 0.7695784272297104 1110 18 0.9355049384369991 1110 19 -1.6151330524920589 1110 20 0.24133480971914878 1110 21 0.21259651027022386 1110 22 0.7909677777687207 1110 23 0.6995117460015621 1110 24 -0.19026726863900478 1110 25 0.22295677214420806 1111 1 0.5832185584029048 1111 2 0.3588136021444903 1111 3 0.9847383372857053 1111 4 0.9684499345238571 1111 5 1.056118664279733 1111 6 1.0856589003205706 1111 7 1.2327645900650666 1111 8 1.553726220221943 1111 9 0.7356858557330099 1111 10 0.7476456790679435 1111 11 0.513117476095232 1111 12 0.6555446201248714 1111 13 0.5855349428559649 1111 14 0.8027430675230249 1111 15 -0.3113253896065089 1111 16 0.21358180936204718 1111 17 0.49107794042303965 1111 18 0.6862339097513299 1111 19 1.1955065842238142 1111 20 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0.4207234747036104 2215 2 -0.10018270452096445 2215 3 0.3041547578464332 2215 4 0.6142994131050763 2215 5 0.8349107321049054 2215 6 1.1776652862751966 2215 7 0.718471827557881 2215 8 -0.6985166601600987 2215 9 -0.09109745234973587 2215 10 -0.20968664889644373 2215 11 -0.17483531810900388 2215 12 -0.5667501345022714 2215 13 -0.211457328370237 2215 14 -0.4080364890441004 2215 15 -0.29486247305911206 2215 16 0.058587429653741196 2215 17 -0.4504640442031942 2215 18 -1.2253808943064541 2215 19 0.11908341698580927 2215 20 -3.8344733272680784 2215 21 -2.1923652317326763 2215 22 -1.1117187088999643 2215 23 -1.170680723745967 2215 24 -1.2383341442920894 2215 25 -1.050444650311718 2216 1 -0.15085762797642843 2216 2 -0.9626195121720826 2216 3 0.16115205925236964 2216 4 -0.08642683096631743 2216 5 0.008558261214886898 2216 6 -1.7084826904303085 2216 7 -9.448063001883813 2216 8 -10.599199284013846 2216 9 -5.721871841422544 2216 10 -3.4086078623197515 2216 11 -2.459040567509227 2216 12 -1.8073774843787334 2216 13 -1.66252293122443 2216 14 -1.8704628799268264 2216 15 -2.891414522721057 2216 16 -1.745170419544003 2216 17 -2.3986666633694007 2216 18 -2.027003305983648 2216 19 -1.5269515377826295 2216 20 -2.286525267879476 2216 21 -4.894487973816744 2216 22 -3.413596656926803 2216 23 -3.1428191235350305 2216 24 -3.4698891399463427 2216 25 -2.9907305254993055 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 35305.0 3 5.0 4 255.0 5 1505.0 6 1917.0 7 1580.0 8 1397.0 9 1141.0 10 1136.0 11 1118.0 12 1070.0 13 1084.0 14 1066.0 15 1087.0 16 1161.0 17 1244.0 18 1503.0 19 1802.0 20 2395.0 21 3053.0 22 3862.0 23 4552.0 24 5365.0 25 6147.0 26 6909.0 27 7610.0 28 8437.0 29 9958.0 30 11893.0 31 14563.0 32 18575.0 33 24555.0 34 33488.0 35 45901.0 36 66399.0 37 104823.0 38 135610.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.25777934998583 18.517853654415763 12.948365284942176 25.276001710656224 2 14.211417449508668 19.647620002074685 42.333247181153425 23.807715367263228 3 19.261593211636992 24.432163843456916 29.200440273025542 27.105802671880554 4 12.150792339836295 16.568846933790685 38.392897935945065 32.88746279042796 5 12.96779304082382 37.55833416761951 35.823370200000355 13.650502591556322 6 32.49736105817615 36.7978263198661 18.050888985293938 12.653923636663816 7 27.77442144327863 31.16694172953189 22.50483928637479 18.55379754081469 8 24.661360686313706 37.17045300438369 20.599450145917395 17.56873616338521 9 25.839000435570668 16.02338030939568 20.045910553455762 38.09170870157789 10 14.458216516649452 29.67459966131945 34.37467062965071 21.492513192380393 11 34.42141553722376 23.02851693330125 23.664997602659728 18.88506992681526 12 22.839509425280088 24.98344670365472 31.71945280159052 20.457591069474667 13 28.716547298317835 21.96260533276196 25.60863807680554 23.712209292114665 14 20.540939267273174 22.416312480241665 27.448101443675576 29.594646808809582 15 23.4023196835901 30.62182553899977 23.170316199147152 22.805538578262976 16 22.04301641831939 28.582418277235654 27.59868906358674 21.77587624085822 17 21.68168326395841 28.718934963204944 27.523578692238793 22.075803080597854 18 21.452682529312174 28.38152542730089 28.93720977611049 21.228582267276444 19 23.662269184899735 27.121356094150954 27.130489460622016 22.08588526032729 20 23.096999000662127 27.761922241326925 27.95406207135167 21.18701668665928 21 22.895364480334955 27.088126508203576 27.140990470436577 22.875518541024896 22 22.539587197212388 28.018040802562805 27.43336564941269 22.009006350812115 23 22.478116394087564 27.585988872028327 27.362410779520037 22.573483954364075 24 22.932407417164573 27.538629601919272 27.752194484546404 21.776768496369748 25 22.791257233681954 27.351264961317913 27.70322365359116 22.154254151408974 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 186.0 1 186.0 2 237.5 3 289.0 4 289.0 5 289.0 6 448.5 7 608.0 8 608.0 9 608.0 10 904.5 11 1201.0 12 1201.0 13 1201.0 14 1963.0 15 2725.0 16 2725.0 17 2725.0 18 4692.5 19 6660.0 20 6660.0 21 6660.0 22 10273.5 23 13887.0 24 13887.0 25 13887.0 26 19802.0 27 25717.0 28 25717.0 29 25717.0 30 33911.0 31 42105.0 32 42105.0 33 42105.0 34 47724.5 35 53344.0 36 53344.0 37 53344.0 38 58071.5 39 62799.0 40 62799.0 41 62799.0 42 66772.0 43 70745.0 44 70745.0 45 70745.0 46 73301.5 47 75858.0 48 75858.0 49 75858.0 50 73081.0 51 70304.0 52 70304.0 53 70304.0 54 62724.5 55 55145.0 56 55145.0 57 55145.0 58 47265.5 59 39386.0 60 39386.0 61 39386.0 62 32179.0 63 24972.0 64 24972.0 65 24972.0 66 19217.0 67 13462.0 68 13462.0 69 13462.0 70 9876.5 71 6291.0 72 6291.0 73 6291.0 74 4367.5 75 2444.0 76 2444.0 77 2444.0 78 1686.0 79 928.0 80 928.0 81 928.0 82 603.5 83 279.0 84 279.0 85 279.0 86 189.0 87 99.0 88 99.0 89 99.0 90 62.5 91 26.0 92 26.0 93 26.0 94 16.0 95 6.0 96 6.0 97 6.0 98 5.5 99 5.0 100 5.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.22266278704271156 2 0.12467711261855302 3 0.12871594866112587 4 0.06971382212614866 5 0.02142339118234291 6 0.020018578645795836 7 0.3023858984917581 8 0.29571303894315953 9 0.018086961408043604 10 0.03564711811488206 11 0.015804141036154604 12 0.017208953572701684 13 0.014926133200812684 14 0.01597974260322299 15 0.014574930066675915 16 0.018789367676317142 17 0.01773575827390684 18 0.014574930066675915 19 0.022828203718889988 20 0.016506547304428146 21 0.014926133200812684 22 0.01668214887149653 23 0.01668214887149653 24 0.01562853946908622 25 0.01510173476788107 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 569471.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 87.35026335772172 #Duplication Level Percentage of deduplicated Percentage of total 1 94.07596200766606 82.17560057000654 2 4.446202103437441 7.767538493538336 3 0.7357189896736555 1.9279574251581226 4 0.27081617764179966 0.9462345775417099 5 0.12703161438254526 0.5548122485535939 6 0.07185023541334475 0.37656821914019806 7 0.048476333123555954 0.2964094325471483 8 0.03669606507877643 0.2564328759058573 9 0.026609251973033207 0.20918926508367844 >10 0.1386869839823408 2.2385459598050095 >50 0.009666183086276574 0.5971966216678045 >100 0.010874408938165834 1.8876275818498052 >500 0.0014096456029957841 0.765886729202219 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 802 0.14083245678884437 No Hit GTCCTACAGTGGACATTTCTAAATT 755 0.1325791831366303 No Hit GTACATGGGAAGCAGTGGTATCAAC 643 0.11291180762497124 No Hit CTGTAGGACGTGGAATATGGCAAGA 592 0.10395612770448363 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 1.7560156706838452E-4 5 0.0 0.0 0.0 0.0 1.7560156706838452E-4 6 0.0 0.0 0.0 0.0 1.7560156706838452E-4 7 0.0 0.0 0.0 0.0 1.7560156706838452E-4 8 0.0 0.0 0.0 0.0 1.7560156706838452E-4 9 0.0 0.0 0.0 0.0 1.7560156706838452E-4 10 0.0 0.0 0.0 0.0 1.7560156706838452E-4 11 0.0 0.0 0.0 0.0 1.7560156706838452E-4 12 0.0 0.0 0.0 0.0 1.7560156706838452E-4 13 0.0 0.0 0.0 0.0 1.7560156706838452E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCTTAAT 35 9.593036E-5 19.08451 1 GCGTCGT 30 7.550128E-4 19.060923 4 AGCGTCG 35 0.0021207905 16.339375 3 CGTCGTC 35 0.0021333355 16.32496 5 TGGTCTA 35 0.002193137 16.25755 10 ATCTAGT 45 6.529457E-4 14.84351 1 TAGCGTC 40 0.005159773 14.296953 2 TAGGACG 300 0.0 14.2956915 4 AGGACGT 295 0.0 13.880828 5 TGTAGAA 210 0.0 13.616146 2 GGACGTG 280 0.0 13.604134 6 GGCGAGG 135 1.8189894E-12 13.342559 19 ACGTGAA 165 0.0 13.262636 8 GACGTGA 155 0.0 12.901984 7 TTAGGAC 185 0.0 12.880138 3 GTCTAGA 105 1.8641003E-8 12.723008 1 AATCCCG 120 7.6033757E-10 12.640319 19 GACGTGG 150 1.2732926E-11 12.062332 7 TGTAGGA 405 0.0 12.00238 2 CTGTAGG 390 0.0 11.744315 1 >>END_MODULE