FastQCFastQC Report
Thu 26 May 2016
SRR1926905_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926905_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences569471
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT14740.2588367098587988No Hit
GGTATCAACGCAGAGTACTTTTTTT9580.16822630125151236No Hit
CTGTAGGACGTGGAATATGGCAAGA7590.13328158940490387No Hit
TATCAACGCAGAGTACTTTTTTTTT7460.13099876903301486No Hit
GTCCTACAGTGGACATTTCTAAATT7400.12994515963060455No Hit
GTACATGGGAAGCAGTGGTATCAAC7130.12520391731975816No Hit
CCCATGTACTCTGCGTTGATACCAC6140.1078193621799881No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG807.852577E-915.41160318
GTCTAAA456.5270835E-414.8442241
TTACCCT602.4852332E-514.2963914
TTGTACC551.9034832E-413.8631664
ACTGTTC2550.013.761198
GTTACAA1600.013.63334215
CCAACGA707.3699302E-613.54866219
GACGTGA1205.2750693E-1113.4369367
GGACGTG2600.013.196676
TAGGACC2200.012.9967184
GATGTAG603.9331656E-412.723621
CCCTTAA603.9331656E-412.723621
GTAGGAC4800.012.7101463
TCTAGCC603.9684994E-412.7101463
CGTTTCC751.5003663E-512.64541814
CACTGTT2800.012.5336127
TGTAGGA5100.012.5320812
GTGCAAG701.0438877E-412.2692061
TCCTATA701.0473386E-412.2648722
ATCCCGT701.10575544E-412.19379610
Produced by FastQC (version 0.11.5)