##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926905_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 569471 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.855845161562222 31.0 30.0 33.0 26.0 34.0 2 30.15920564875121 31.0 30.0 34.0 26.0 34.0 3 30.22417647255084 31.0 30.0 34.0 26.0 34.0 4 33.806987537556786 35.0 35.0 37.0 28.0 37.0 5 33.77002516370456 37.0 35.0 37.0 29.0 37.0 6 33.711365460225366 37.0 35.0 37.0 29.0 37.0 7 33.70224998287885 37.0 35.0 37.0 29.0 37.0 8 33.734118155270416 37.0 35.0 37.0 29.0 37.0 9 35.25961111276957 39.0 35.0 39.0 29.0 39.0 10 35.16019077354246 39.0 35.0 39.0 29.0 39.0 11 35.18873305225376 39.0 35.0 39.0 29.0 39.0 12 35.079303423703756 39.0 35.0 39.0 29.0 39.0 13 35.09805240301964 39.0 35.0 39.0 29.0 39.0 14 36.20068273889276 40.0 36.0 41.0 29.0 41.0 15 36.162101318592164 40.0 36.0 41.0 29.0 41.0 16 36.13859353680872 40.0 36.0 41.0 28.0 41.0 17 36.11872948754195 40.0 36.0 41.0 28.0 41.0 18 36.08948831459372 40.0 36.0 41.0 28.0 41.0 19 36.157807860277345 40.0 37.0 41.0 28.0 41.0 20 36.12402036275772 40.0 36.0 41.0 28.0 41.0 21 36.07061817019655 40.0 36.0 41.0 28.0 41.0 22 35.98498255398431 40.0 36.0 41.0 27.0 41.0 23 35.91144237371174 40.0 36.0 41.0 27.0 41.0 24 35.81894424825847 40.0 36.0 41.0 27.0 41.0 25 35.70942155087792 40.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.09125300605601083 1101 2 0.20749881021396277 1101 3 0.5035115860791386 1101 4 0.139096910335887 1101 5 0.07873666304384841 1101 6 0.18157834238461845 1101 7 0.22070212190028116 1101 8 0.20508462854181886 1101 9 0.3676804587594802 1101 10 0.1282324834099171 1101 11 0.30025036302520647 1101 12 0.5175212324542926 1101 13 0.4838028354721686 1101 14 0.32403714262773775 1101 15 0.2972777655835941 1101 16 0.5362670784693933 1101 17 0.32397706425248884 1101 18 0.2728806385443576 1101 19 0.17556529366586915 1101 20 0.23458979136014335 1101 21 0.3825819794628629 1101 22 0.3843322880894604 1101 23 0.31819894477410315 1101 24 0.4211755714249108 1101 25 0.5637133887571721 1102 1 -0.423850455492758 1102 2 -0.45822863003714787 1102 3 -0.15380930813954663 1102 4 -0.19636189237584745 1102 5 -0.15967140453537354 1102 6 -0.27299785047988934 1102 7 -0.1434124787500366 1102 8 -0.09716078004984752 1102 9 -0.023679691366467637 1102 10 -0.18227436073297554 1102 11 -0.15337204541430793 1102 12 -0.19152098201617918 1102 13 0.004312722708647243 1102 14 -0.3363085302622437 1102 15 -0.5179712327314334 1102 16 -0.29718271893946735 1102 17 -0.4033575238873368 1102 18 -0.25256300505303386 1102 19 -0.33811453907669176 1102 20 -0.1350995876797043 1102 21 -0.1959594206983084 1102 22 -0.18618577041682727 1102 23 -0.023405216374605686 1102 24 -0.19400129430140112 1102 25 -0.23436748990792466 1103 1 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0.6328351811950341 2211 13 0.5050083945019708 2211 14 0.47002111054289486 2211 15 0.7820403077698899 2211 16 0.61768598516597 2211 17 0.6621276893590817 2211 18 0.5838808607815054 2211 19 0.6137135930348165 2211 20 0.48153273843152533 2211 21 0.5753817541247273 2211 22 0.4584199528938129 2211 23 0.6657468420488613 2211 24 0.6016747780899578 2211 25 0.40143762862858523 2212 1 0.17602486630399383 2212 2 0.29048065553879 2212 3 0.07559730314023838 2212 4 0.35090230612416917 2212 5 0.2813969799198759 2212 6 0.39437301714956874 2212 7 0.1215620532898285 2212 8 0.0790998230488924 2212 9 0.17498450739007865 2212 10 0.07117000290152475 2212 11 0.17447005964631757 2212 12 0.2112550320524491 2212 13 0.07346423109684252 2212 14 0.15291488615141446 2212 15 0.11922160930307513 2212 16 0.12932576546356955 2212 17 0.3204302307120983 2212 18 0.0943630743690349 2212 19 0.2666812105766567 2212 20 0.2538014868360463 2212 21 -0.016581862518350476 2212 22 0.11567786087432097 2212 23 0.15890202304218093 2212 24 0.258712701339995 2212 25 0.12841657950140473 2213 1 0.1144311202442303 2213 2 0.04737694156128924 2213 3 0.02138668096845109 2213 4 0.19236828351247226 2213 5 0.2035904822104655 2213 6 -0.12690424927333055 2213 7 -0.04025374687549288 2213 8 -0.14121007201828206 2213 9 -0.25760420917922033 2213 10 -0.1737514726024756 2213 11 -0.3048305749941207 2213 12 -0.17426802094633587 2213 13 -0.42922213324000325 2213 14 -0.2765916914134152 2213 15 -0.385279014129587 2213 16 -0.20741893535880251 2213 17 -0.3091141731041489 2213 18 -0.3022101474469068 2213 19 -0.31527916992740757 2213 20 -0.3055612108761636 2213 21 -0.28146369050697473 2213 22 -0.4087936223350255 2213 23 -0.447988659091223 2213 24 -0.37316081888304353 2213 25 -0.09722401507122669 2214 1 0.09922916118634006 2214 2 0.10851495608913453 2214 3 0.03624011849138142 2214 4 0.08298296544071349 2214 5 0.0021542066216468925 2214 6 0.09890101890482583 2214 7 0.15539065109941674 2214 8 0.14394015910652058 2214 9 0.16407474745737005 2214 10 -0.03574153056710827 2214 11 -0.12360396262344153 2214 12 -0.10908532228906154 2214 13 0.061881395999115796 2214 14 -0.11131911088659763 2214 15 -0.03943100620530515 2214 16 -0.21368773717844647 2214 17 -0.08038774416586136 2214 18 0.02104816204021631 2214 19 -0.0703831760907363 2214 20 -0.09419665735059368 2214 21 -0.061509915468455745 2214 22 0.11228734472051372 2214 23 0.13631686589226888 2214 24 -0.11506227900483879 2214 25 0.04556434064085835 2215 1 0.18556113965011178 2215 2 0.15075057941768577 2215 3 0.18939683173722344 2215 4 0.29118900302285056 2215 5 0.24057361402118005 2215 6 0.2090040952557075 2215 7 -0.036036699554991 2215 8 0.2250386218166156 2215 9 0.052852575415499814 2215 10 0.09110149052634853 2215 11 -0.05882370829759509 2215 12 0.08297186560389491 2215 13 0.0743231720294375 2215 14 0.36361580224235723 2215 15 0.5043686944836452 2215 16 0.25421712972553934 2215 17 0.33295927069342923 2215 18 0.16577014811959856 2215 19 -0.16977237786889532 2215 20 0.09968584092261068 2215 21 0.009027427570345026 2215 22 -0.06706351336353578 2215 23 -0.01908135949426537 2215 24 0.0868332100765059 2215 25 -0.020048317205876742 2216 1 -0.12828307106809334 2216 2 0.09788873506373719 2216 3 -1.9211966961943645 2216 4 -1.2816779195056895 2216 5 -0.2630283091746861 2216 6 0.13261012005412454 2216 7 0.055324869731165904 2216 8 0.07058073323046443 2216 9 -0.05082682249528858 2216 10 -0.1700664245249044 2216 11 -0.21651079508555426 2216 12 -0.04349095792628077 2216 13 -0.11055819074428541 2216 14 -0.27037801931189165 2216 15 -0.17059883449613267 2216 16 -0.24676432517147617 2216 17 -0.4164458341741337 2216 18 -0.21487484515833444 2216 19 0.07745659897658186 2216 20 0.08995267295547649 2216 21 -0.058108644722025815 2216 22 0.06307122102514029 2216 23 -0.28740754757553333 2216 24 -0.18550994431627998 2216 25 -0.4594308632593851 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 13844.0 3 9.0 4 207.0 5 1582.0 6 2063.0 7 1548.0 8 1144.0 9 980.0 10 924.0 11 892.0 12 774.0 13 759.0 14 710.0 15 733.0 16 778.0 17 797.0 18 935.0 19 1025.0 20 1367.0 21 1801.0 22 2232.0 23 2753.0 24 3305.0 25 3820.0 26 4632.0 27 5028.0 28 5870.0 29 7213.0 30 9231.0 31 12038.0 32 15369.0 33 20241.0 34 27050.0 35 38168.0 36 56199.0 37 96208.0 38 227242.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.96725691874702 19.206016317045002 10.73054022744013 28.09618653676785 2 14.182828795983623 20.668130384568734 40.0554783070327 25.09356251241494 3 18.783545495419546 24.217915753386162 29.228664362389704 27.76987438880459 4 12.175159051119373 17.286042660644704 37.54045421101338 32.99834407722255 5 13.150325052770587 38.20379807742964 35.1922745967133 13.453602273086474 6 31.984959862425733 37.74323933834671 17.3830231099396 12.888777689287956 7 28.17878288115976 31.947569292592924 21.056236728543052 18.817411097704266 8 25.218687820392365 37.978184781329745 19.488812280587755 17.31431511769014 9 25.829355587635096 16.228851212992915 19.654629815004093 38.2871633843679 10 14.784187632693127 30.091157654802064 33.46579963579084 21.65885507671397 11 34.439158540247504 23.152611605457302 23.441843639090404 18.96638621520479 12 22.87819508734468 25.227229624842835 31.322216524896923 20.572358762915563 13 28.867996094600574 21.99473894933517 25.48589209578062 23.651372860283633 14 20.827472610449384 22.314998709323948 27.232913468511892 29.624615211714772 15 23.07262746420255 30.62881286284757 23.157796766727426 23.140762906222452 16 22.148195496115648 28.805859545116107 27.193466136577555 21.85247882219069 17 21.635517875361522 28.760024654460015 27.446876135922636 22.157581334255827 18 21.41173867539528 28.322223548996604 28.99390308147253 21.27213469413558 19 23.06126749433684 27.33963158726535 27.37369835109839 22.22540256729942 20 22.99641594684152 27.862193946269052 27.97809179460794 21.163298312281484 21 22.860620238358674 27.05841649191948 27.212088641608446 22.868874628113396 22 22.54232436770266 28.065344855137486 27.335369140834214 22.056961636325642 23 22.38428295734111 27.641442672234405 27.3099069136093 22.664367456815185 24 22.67349873830274 27.290590741231775 28.070086097448332 21.965824423017153 25 22.674796376753058 27.389477972864285 27.76950445530123 22.16622119508143 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 215.0 1 215.0 2 207.0 3 199.0 4 199.0 5 199.0 6 356.0 7 513.0 8 513.0 9 513.0 10 831.0 11 1149.0 12 1149.0 13 1149.0 14 1826.5 15 2504.0 16 2504.0 17 2504.0 18 4399.0 19 6294.0 20 6294.0 21 6294.0 22 9791.0 23 13288.0 24 13288.0 25 13288.0 26 19143.0 27 24998.0 28 24998.0 29 24998.0 30 33130.0 31 41262.0 32 41262.0 33 41262.0 34 46890.0 35 52518.0 36 52518.0 37 52518.0 38 57434.0 39 62350.0 40 62350.0 41 62350.0 42 66358.5 43 70367.0 44 70367.0 45 70367.0 46 73500.5 47 76634.0 48 76634.0 49 76634.0 50 74048.5 51 71463.0 52 71463.0 53 71463.0 54 63826.0 55 56189.0 56 56189.0 57 56189.0 58 48388.0 59 40587.0 60 40587.0 61 40587.0 62 32986.5 63 25386.0 64 25386.0 65 25386.0 66 19608.5 67 13831.0 68 13831.0 69 13831.0 70 9963.5 71 6096.0 72 6096.0 73 6096.0 74 4281.0 75 2466.0 76 2466.0 77 2466.0 78 1644.0 79 822.0 80 822.0 81 822.0 82 538.0 83 254.0 84 254.0 85 254.0 86 163.5 87 73.0 88 73.0 89 73.0 90 41.0 91 9.0 92 9.0 93 9.0 94 6.5 95 4.0 96 4.0 97 4.0 98 2.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.10852176844826163 2 0.10536094024103072 3 0.01668214887149653 4 0.0 5 0.004390039176709613 6 0.3376818134725034 7 0.019316172377522294 8 0.009131281487555995 9 0.00930688305462438 10 0.0031608282072309213 11 0.005619250146188305 12 5.268047012051535E-4 13 5.268047012051535E-4 14 3.5120313413676904E-4 15 0.002985226640162537 16 0.0012292109694786917 17 0.0 18 5.268047012051535E-4 19 1.7560156706838452E-4 20 7.024062682735381E-4 21 0.013521320664265608 22 0.0 23 0.0 24 0.0 25 0.006497257981530227 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 569471.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 84.75229701767003 #Duplication Level Percentage of deduplicated Percentage of total 1 92.63098769865054 78.5068898247617 2 5.55301200994134 9.412610464184747 3 0.9207119760501016 2.340973645857723 4 0.32960121456684177 1.1173784013741506 5 0.15752311509459832 0.6675222918823008 6 0.08981765028025063 0.4567351304388609 7 0.06110575776332542 0.3625197332013007 8 0.03934613525992587 0.26677402736373257 9 0.03189172016854107 0.24326068861157493 >10 0.15922269628396726 2.503340936965533 >50 0.012248254227488814 0.715386399730709 >100 0.01204061084802954 1.8876924966776059 >500 0.002283564126291416 1.2595759701288989 >1k 2.0759673875376508E-4 0.25933998882106396 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 1474 0.2588367098587988 No Hit GGTATCAACGCAGAGTACTTTTTTT 958 0.16822630125151236 No Hit CTGTAGGACGTGGAATATGGCAAGA 759 0.13328158940490387 No Hit TATCAACGCAGAGTACTTTTTTTTT 746 0.13099876903301486 No Hit GTCCTACAGTGGACATTTCTAAATT 740 0.12994515963060455 No Hit GTACATGGGAAGCAGTGGTATCAAC 713 0.12520391731975816 No Hit CCCATGTACTCTGCGTTGATACCAC 614 0.1078193621799881 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCCAACG 80 7.852577E-9 15.411603 18 GTCTAAA 45 6.5270835E-4 14.844224 1 TTACCCT 60 2.4852332E-5 14.296391 4 TTGTACC 55 1.9034832E-4 13.863166 4 ACTGTTC 255 0.0 13.76119 8 GTTACAA 160 0.0 13.633342 15 CCAACGA 70 7.3699302E-6 13.548662 19 GACGTGA 120 5.2750693E-11 13.436936 7 GGACGTG 260 0.0 13.19667 6 TAGGACC 220 0.0 12.996718 4 GATGTAG 60 3.9331656E-4 12.72362 1 CCCTTAA 60 3.9331656E-4 12.72362 1 GTAGGAC 480 0.0 12.710146 3 TCTAGCC 60 3.9684994E-4 12.710146 3 CGTTTCC 75 1.5003663E-5 12.645418 14 CACTGTT 280 0.0 12.533612 7 TGTAGGA 510 0.0 12.532081 2 GTGCAAG 70 1.0438877E-4 12.269206 1 TCCTATA 70 1.0473386E-4 12.264872 2 ATCCCGT 70 1.10575544E-4 12.193796 10 >>END_MODULE