FastQCFastQC Report
Thu 26 May 2016
SRR1926904_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926904_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences538038
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT14530.2700552749062334No Hit
CTGTAGGACGTGGAATATGGCAAGA12100.22489117868998101No Hit
GTCCTAAAGTGTGTATTTCTCATTT11360.2111375032990235No Hit
CTTTAGGACGTGAAATATGGCGAGG10320.1918080135603805No Hit
GTATCAACGCAGAGTACTTTTTTTT9050.1682037328218453No Hit
GTCCTACAGTGTGCATTTCTCATTT8410.1563086622134496No Hit
GTACATGGGAAGCAGTGGTATCAAC5620.10445358877997465No Hit
CTGTAGGACCTGGAATATGGCGAGA5530.10278084447566901No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGTTT704.5214802E-714.89876712
GTACTAG400.00514881314.3013291
GAATCAC400.00534024114.22287418
TGCCGGT759.806972E-713.91198310
GCCGGTT759.858541E-713.90551611
GCCCTAT500.001457279413.3479061
TAAGACT500.001468616213.3341924
TCCAACG1001.0286385E-813.27468218
CGGTTTC802.0355583E-613.03763413
GTAGCAC603.9747302E-412.707543
CCAACGA751.5034957E-512.64255319
AGGACCT4300.012.6206835
TGTAGGA7300.012.2723492
CCTTATA701.0560797E-412.2536982
CCACCTT1405.4569682E-1112.19103413
TTAGGCT550.003005571412.1219934
ACTGACC550.003033021212.1072938
CCAGTAC802.7654385E-511.9133183
TAGGACC2800.011.9055294
GTTATAG657.770367E-411.7344231
Produced by FastQC (version 0.11.5)