##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926904_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 538038 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.139975243384296 31.0 28.0 31.0 16.0 34.0 2 28.356058494009716 31.0 28.0 33.0 16.0 34.0 3 27.8197264877202 31.0 28.0 31.0 16.0 34.0 4 31.53915522695423 35.0 32.0 37.0 19.0 37.0 5 31.445070794256168 35.0 32.0 37.0 18.0 37.0 6 31.191544091681255 35.0 32.0 37.0 16.0 37.0 7 31.302480493942806 35.0 32.0 37.0 17.0 37.0 8 30.932737092919087 35.0 32.0 37.0 11.0 37.0 9 33.11236009352499 37.0 33.0 39.0 17.0 39.0 10 33.258693623870435 37.0 33.0 39.0 17.0 39.0 11 33.42099256929808 38.0 34.0 39.0 18.0 39.0 12 33.28518431783629 38.0 33.0 39.0 17.0 39.0 13 33.39413758879484 38.0 34.0 39.0 18.0 39.0 14 34.40611815522324 39.0 34.0 41.0 17.0 41.0 15 33.975544478271054 39.0 33.0 41.0 13.0 41.0 16 34.05759630360681 39.0 33.0 41.0 17.0 41.0 17 34.284777283388905 39.0 34.0 41.0 17.0 41.0 18 34.339972269616645 39.0 34.0 41.0 17.0 41.0 19 33.71009296741122 39.0 33.0 41.0 10.0 41.0 20 33.995517045264464 39.0 34.0 41.0 11.0 41.0 21 33.97950516506269 39.0 34.0 41.0 10.0 41.0 22 34.0082280433724 39.0 34.0 41.0 10.0 41.0 23 34.00866295689152 39.0 34.0 41.0 2.0 41.0 24 32.69902497593107 38.0 32.0 40.0 2.0 41.0 25 33.27855095736732 39.0 33.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.10877660097609265 1101 2 -0.025241539732949292 1101 3 -0.8175126368839649 1101 4 -0.25645606450917313 1101 5 -0.12711717861085248 1101 6 -1.7877626301025984 1101 7 -0.3360569425594946 1101 8 0.18015701208346968 1101 9 0.28405557876694587 1101 10 -8.558913539492035 1101 11 -3.3320047777995434 1101 12 -1.1247362329116655 1101 13 -0.26982671726542407 1101 14 0.2051217570466335 1101 15 -1.2157021532853491 1101 16 0.378125833958741 1101 17 0.37922141640679286 1101 18 0.3237066030332585 1101 19 -0.22686299858364833 1101 20 0.5236089106564208 1101 21 0.443072339683539 1101 22 0.6520405609801045 1101 23 0.6483863736386937 1101 24 0.40923603185321156 1101 25 0.36259825341288376 1102 1 -0.42606198478674173 1102 2 -0.5470365664210526 1102 3 -0.6747707788467103 1102 4 -0.5893479521310354 1102 5 -0.6711827542095925 1102 6 -1.5507635935385018 1102 7 -0.45232599829937215 1102 8 -0.0016349786972860159 1102 9 -0.1599706539508432 1102 10 -0.1277744236845635 1102 11 -0.3436821785671924 1102 12 -0.05005291212468421 1102 13 -0.4118854961491394 1102 14 -1.0057065190226737 1102 15 -0.20922916511609202 1102 16 -0.4319905528158543 1102 17 -0.5017834069684568 1102 18 -0.7621522644493837 1102 19 -0.08062829816264383 1102 20 -0.29215953015732765 1102 21 -0.41064649192583147 1102 22 -0.3248772495730847 1102 23 -0.526678604072174 1102 24 -3.4734633234811554 1102 25 -1.7653203223128706 1103 1 -0.7830828209776222 1103 2 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24 -4.786507769092989 1105 25 -2.2840280838398996 1106 1 -0.24086679032933134 1106 2 -0.6962731091820267 1106 3 -0.3557924480111474 1106 4 -0.7104600323216843 1106 5 -0.1334582621574114 1106 6 0.02687810363369536 1106 7 -2.6932326882239472 1106 8 -0.5175336839524256 1106 9 -1.049656303657251 1106 10 -0.22834747726783178 1106 11 -0.5307384152082264 1106 12 -0.15335917947598432 1106 13 -0.46994939807282776 1106 14 -0.9484656522560968 1106 15 -0.21483071782719776 1106 16 -0.18095254578934572 1106 17 -0.5902412601979492 1106 18 -0.9677329341788905 1106 19 -0.22313622141154354 1106 20 -0.39286770891564515 1106 21 -0.5605617315887557 1106 22 -1.0357738665300644 1106 23 -1.2220880333525699 1106 24 -4.269771335175626 1106 25 -2.5635692228853095 1107 1 0.3029907507645859 1107 2 0.07523510960101731 1107 3 0.4873777913428867 1107 4 0.3575852430796438 1107 5 0.4578615494353997 1107 6 0.3735208989811909 1107 7 -1.9169782423910142 1107 8 -1.6431585037159557 1107 9 -0.243458572233628 1107 10 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0.5750548125693768 2215 4 0.8871048371751868 2215 5 0.9487843612229021 2215 6 1.557386143412316 2215 7 0.8746237528777172 2215 8 -0.19373064488335956 2215 9 0.3094524434908905 2215 10 0.126599470017851 2215 11 0.029920158918201878 2215 12 -0.21963277210536347 2215 13 0.22456494035109387 2215 14 0.3836609865378833 2215 15 0.027743539598546363 2215 16 0.31812503561819483 2215 17 -0.042504733513304416 2215 18 -0.3862025926111059 2215 19 0.7739546122583718 2215 20 -3.127106085895573 2215 21 -1.622489313542097 2215 22 -0.6522483171724787 2215 23 -0.3381299297141993 2215 24 -0.3114480083556259 2215 25 -0.21818486361145517 2216 1 -0.21580070906057003 2216 2 -0.7673328735295044 2216 3 0.06012193754505546 2216 4 -0.1521125120492961 2216 5 0.0581998176777212 2216 6 -1.8520699721693603 2216 7 -10.116864021945556 2216 8 -11.33308294249819 2216 9 -6.092973135340422 2216 10 -3.4640392025489604 2216 11 -2.1485646774724607 2216 12 -2.017685374538928 2216 13 -1.7588421448135634 2216 14 -1.8215728159136333 2216 15 -2.787867320129962 2216 16 -1.725305397364096 2216 17 -2.1516520392849046 2216 18 -1.7635754143545057 2216 19 -1.186634720582731 2216 20 -2.083467368429112 2216 21 -5.060113722986991 2216 22 -3.538422717388002 2216 23 -3.5354364496421624 2216 24 -3.340271088770894 2216 25 -2.781631809686978 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 30974.0 3 18.0 4 242.0 5 1408.0 6 1764.0 7 1468.0 8 1280.0 9 1065.0 10 1023.0 11 1019.0 12 996.0 13 910.0 14 1031.0 15 1038.0 16 1092.0 17 1253.0 18 1345.0 19 1719.0 20 2256.0 21 2866.0 22 3613.0 23 4200.0 24 4817.0 25 5696.0 26 6691.0 27 7137.0 28 8013.0 29 9547.0 30 11386.0 31 14029.0 32 17981.0 33 23077.0 34 31372.0 35 43723.0 36 63036.0 37 98793.0 38 130160.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.569525668437244 19.032116277163993 12.95993473936721 25.43842331503155 2 13.708409246132403 19.95314032407244 43.363996211028585 22.974454218766574 3 18.848344874946726 25.216858882141548 29.568180506987314 26.36661573592441 4 11.985611537553032 17.056354191272067 38.823739475088956 32.134294796085946 5 12.509946086633203 38.189998140918384 36.0381111730805 13.261944599367911 6 31.587479526977912 37.385550926468156 18.55594781958829 12.471021726965642 7 27.19330293732781 31.37449345168638 23.236540820244784 18.195662790741025 8 24.060259693780548 37.964091724171354 21.002911582581206 16.972736999466893 9 26.088427533591606 15.753478134782837 20.53382211878081 37.624272212844744 10 14.701928636901984 29.51838926374151 34.8225590834466 20.957123015909907 11 33.70437912105276 22.70015745279669 25.148948114934967 18.446515311215585 12 23.345013802782862 24.79258646490747 32.247204587914894 19.615195144394768 13 28.88837664957068 22.711142258566426 25.65197427980288 22.74850681206001 14 20.111687186646616 23.25800149089753 27.602053051413655 29.028258271042194 15 22.63059077220508 32.333345726289174 23.136966947986764 21.89909655351898 16 21.11518234090136 28.499538036254396 29.28720016508 21.098079457764243 17 20.956599978435694 29.37969163044731 28.45372783617077 21.20998055494622 18 20.74951900287204 28.00453577968008 30.921934398497985 20.32401081894989 19 23.641001234439372 26.87080773978613 27.457724169727975 22.03046685604652 20 23.219261735110244 28.054586317728656 28.3997940279252 20.326357919235896 21 22.337227783509466 27.27546496389036 27.15351941183578 23.233787840764393 22 22.5554293532514 29.13333717517107 27.457304479892624 20.8539289916849 23 21.594696737326025 28.65524145200239 28.116698547960432 21.633363262711157 24 23.215241735199204 27.29986764421575 28.603534940439896 20.881355680145145 25 22.18832780302819 27.857680617907032 28.50365743709859 21.450334141966188 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 196.0 1 196.0 2 271.0 3 346.0 4 346.0 5 346.0 6 485.0 7 624.0 8 624.0 9 624.0 10 910.5 11 1197.0 12 1197.0 13 1197.0 14 1981.0 15 2765.0 16 2765.0 17 2765.0 18 4782.5 19 6800.0 20 6800.0 21 6800.0 22 10460.0 23 14120.0 24 14120.0 25 14120.0 26 20700.5 27 27281.0 28 27281.0 29 27281.0 30 36112.5 31 44944.0 32 44944.0 33 44944.0 34 50244.5 35 55545.0 36 55545.0 37 55545.0 38 59112.5 39 62680.0 40 62680.0 41 62680.0 42 65469.0 43 68258.0 44 68258.0 45 68258.0 46 69550.5 47 70843.0 48 70843.0 49 70843.0 50 66889.0 51 62935.0 52 62935.0 53 62935.0 54 55178.0 55 47421.0 56 47421.0 57 47421.0 58 39954.5 59 32488.0 60 32488.0 61 32488.0 62 26529.5 63 20571.0 64 20571.0 65 20571.0 66 15806.0 67 11041.0 68 11041.0 69 11041.0 70 8070.0 71 5099.0 72 5099.0 73 5099.0 74 3486.0 75 1873.0 76 1873.0 77 1873.0 78 1273.5 79 674.0 80 674.0 81 674.0 82 450.0 83 226.0 84 226.0 85 226.0 86 151.5 87 77.0 88 77.0 89 77.0 90 50.0 91 23.0 92 23.0 93 23.0 94 14.0 95 5.0 96 5.0 97 5.0 98 5.5 99 6.0 100 6.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.20704857277738745 2 0.12805786951850984 3 0.13140335812712114 4 0.07155628412863033 5 0.025648745999353205 6 0.025091164564584656 7 0.2906857879926697 8 0.2899423460796449 9 0.01970121069515536 10 0.03661451421646798 11 0.018400187347362082 12 0.01914362926038681 13 0.01765674543433735 14 0.019887071173411545 15 0.018214326869105898 16 0.02174567595597337 17 0.020816373564692455 18 0.017285024477824987 19 0.026392187912377938 20 0.018771908303874447 21 0.017285024477824987 22 0.018771908303874447 23 0.01951535021689918 24 0.017470884956081168 25 0.017285024477824987 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 538038.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 84.29239124600817 #Duplication Level Percentage of deduplicated Percentage of total 1 93.72369906701917 79.00194710780309 2 4.616537011891865 7.782778880161333 3 0.7603625968154895 1.92278344498806 4 0.3013537108187212 1.016072995830722 5 0.1543962157390851 0.6507213111991024 6 0.09271837300697941 0.46892720239185787 7 0.06388252246778521 0.376936740434552 8 0.043219279372475404 0.29144451249881814 9 0.03051296543721621 0.23148097386387778 >10 0.17606043173822133 2.7819439221253175 >50 0.020943760141749607 1.2523183517056937 >100 0.01432992244366232 2.6936246469956635 >500 0.0011023017264353715 0.631268915201341 >1k 8.818413811482971E-4 0.8977509948006118 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 1453 0.2700552749062334 No Hit CTGTAGGACGTGGAATATGGCAAGA 1210 0.22489117868998101 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1136 0.2111375032990235 No Hit CTTTAGGACGTGAAATATGGCGAGG 1032 0.1918080135603805 No Hit GTATCAACGCAGAGTACTTTTTTTT 905 0.1682037328218453 No Hit GTCCTACAGTGTGCATTTCTCATTT 841 0.1563086622134496 No Hit GTACATGGGAAGCAGTGGTATCAAC 562 0.10445358877997465 No Hit CTGTAGGACCTGGAATATGGCGAGA 553 0.10278084447566901 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 1.8586047825618266E-4 0.0 0.0 0.0 0.0 6 1.8586047825618266E-4 0.0 0.0 0.0 0.0 7 1.8586047825618266E-4 0.0 0.0 0.0 0.0 8 1.8586047825618266E-4 0.0 0.0 0.0 0.0 9 1.8586047825618266E-4 0.0 0.0 1.8586047825618266E-4 0.0 10 1.8586047825618266E-4 0.0 0.0 1.8586047825618266E-4 0.0 11 1.8586047825618266E-4 0.0 0.0 1.8586047825618266E-4 0.0 12 1.8586047825618266E-4 0.0 0.0 1.8586047825618266E-4 0.0 13 1.8586047825618266E-4 0.0 0.0 1.8586047825618266E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCGGTTT 70 4.5214802E-7 14.898767 12 GTACTAG 40 0.005148813 14.301329 1 GAATCAC 40 0.005340241 14.222874 18 TGCCGGT 75 9.806972E-7 13.911983 10 GCCGGTT 75 9.858541E-7 13.905516 11 GCCCTAT 50 0.0014572794 13.347906 1 TAAGACT 50 0.0014686162 13.334192 4 TCCAACG 100 1.0286385E-8 13.274682 18 CGGTTTC 80 2.0355583E-6 13.037634 13 GTAGCAC 60 3.9747302E-4 12.70754 3 CCAACGA 75 1.5034957E-5 12.642553 19 AGGACCT 430 0.0 12.620683 5 TGTAGGA 730 0.0 12.272349 2 CCTTATA 70 1.0560797E-4 12.253698 2 CCACCTT 140 5.4569682E-11 12.191034 13 TTAGGCT 55 0.0030055714 12.121993 4 ACTGACC 55 0.0030330212 12.107293 8 CCAGTAC 80 2.7654385E-5 11.913318 3 TAGGACC 280 0.0 11.905529 4 GTTATAG 65 7.770367E-4 11.734423 1 >>END_MODULE