FastQCFastQC Report
Thu 26 May 2016
SRR1926904_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926904_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences538038
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT14770.27451592638438177No Hit
GTATCAACGCAGAGTACTTTTTTTT14440.26838253060192774No Hit
CTGTAGGACGTGGAATATGGCAAGA14370.26708150725413443No Hit
GTCCTAAAGTGTGTATTTCTCATTT12070.22433359725521246No Hit
CTTTAGGACGTGAAATATGGCGAGG11720.21782848051624606No Hit
GGTATCAACGCAGAGTACTTTTTTT9320.17322196573476223No Hit
GTCCTACAGTGTGCATTTCTCATTT8900.16541582564800256No Hit
TATCAACGCAGAGTACTTTTTTTTT7870.14627219638761574No Hit
CTGTAGGACCTGGAATATGGCGAGA7250.13474884673573242No Hit
GTACATGGGAAGCAGTGGTATCAAC7060.13121749764886495No Hit
CTGAAGGACCTGGAATATGGCGAGA6020.11188800791022196No Hit
CCCATGTACTCTGCGTTGATACCAC5990.11133042647545341No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAACGA402.7805803E-416.60637519
TAGGACC3200.014.8755134
TCCAACG801.3007775E-714.23403618
ATAGGAC551.9212326E-413.8477513
AGGACCC500.001473388813.328465
TAAGACT1009.71886E-913.328464
GACGTGG4100.012.7319937
GTTTAGG603.9892315E-412.7020631
ACTACAC604.011276E-412.6937713
CTAAGAC751.442264E-512.693773
AGGACCT5200.012.6327435
GGACCTG5050.012.630936
CTGTAGG8450.012.5141611
GTAGGAC8650.012.436963
GGACGTG8000.012.3764276
TGTAGGA9200.012.3223812
TCCTATA550.003000564212.1246972
CGTCAAG550.0030152812.1167813
AGGACGT8250.012.0013845
TAGGACG8250.012.0013844