##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926904_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 538038 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.837273575472363 31.0 30.0 33.0 25.0 34.0 2 30.080299161025803 31.0 30.0 33.0 26.0 34.0 3 30.177911225601164 31.0 30.0 34.0 26.0 34.0 4 33.77618123626956 35.0 35.0 37.0 28.0 37.0 5 33.76700158724849 37.0 35.0 37.0 28.0 37.0 6 33.748006646370705 37.0 35.0 37.0 29.0 37.0 7 33.73621379902535 37.0 35.0 37.0 29.0 37.0 8 33.76568569506243 37.0 35.0 37.0 29.0 37.0 9 35.29478029432865 39.0 35.0 39.0 30.0 39.0 10 35.17327586527345 39.0 35.0 39.0 29.0 39.0 11 35.216546043216276 39.0 35.0 39.0 29.0 39.0 12 35.105373598147345 39.0 35.0 39.0 29.0 39.0 13 35.127292124348095 39.0 35.0 39.0 29.0 39.0 14 36.20257676967054 40.0 36.0 41.0 29.0 41.0 15 36.19682810507808 40.0 36.0 41.0 29.0 41.0 16 36.1877990030444 40.0 36.0 41.0 29.0 41.0 17 36.16051654344117 40.0 36.0 41.0 29.0 41.0 18 36.13227504377014 40.0 36.0 41.0 29.0 41.0 19 36.19633929202027 40.0 37.0 41.0 29.0 41.0 20 36.16192536586635 40.0 37.0 41.0 29.0 41.0 21 36.102239990483945 40.0 36.0 41.0 28.0 41.0 22 36.01978298930559 40.0 36.0 41.0 28.0 41.0 23 35.94525107892007 40.0 36.0 41.0 28.0 41.0 24 35.87640278195964 40.0 36.0 41.0 27.0 41.0 25 35.75558789527877 40.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.07367026126534526 1101 2 -0.040661622979577317 1101 3 0.3590302949412383 1101 4 0.16040848488795234 1101 5 -0.09221112837803958 1101 6 0.030403555919654934 1101 7 -0.05337191140533548 1101 8 0.048398924135462096 1101 9 0.2837450163540751 1101 10 0.1419474969678589 1101 11 0.09647607710905248 1101 12 0.09254635522167831 1101 13 -0.021561847778400534 1101 14 0.19020107851566337 1101 15 0.22557955980962419 1101 16 0.36561496712081976 1101 17 0.25945113887085824 1101 18 0.23736261902858047 1101 19 0.21371747413581232 1101 20 0.22092001880020717 1101 21 0.1956052797669372 1101 22 0.18700367305066123 1101 23 0.2133046831511507 1101 24 0.4568311257493676 1101 25 0.3154718517293418 1102 1 -0.59978061339962 1102 2 -0.5745321260418166 1102 3 -0.2735025501686259 1102 4 -0.3455549045477042 1102 5 -0.6164534681332512 1102 6 -0.6928843317393785 1102 7 -0.499473713433197 1102 8 -0.5288432328457304 1102 9 -0.7177078821629763 1102 10 -0.47561336832769285 1102 11 -0.7170835862150184 1102 12 -0.5417336855462196 1102 13 -0.5596733275353998 1102 14 -0.5977764185955792 1102 15 -0.4013322099075083 1102 16 -0.5921348059605975 1102 17 -0.6491138372910754 1102 18 -0.5939152811148531 1102 19 -0.39817429886505806 1102 20 -0.21851517851828106 1102 21 -0.27545291981608955 1102 22 -0.42623102427247517 1102 23 -0.46529960491309197 1102 24 -0.4114275335567754 1102 25 -0.5127995707791229 1103 1 -1.0422067640037582 1103 2 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1110 9 0.6233784412792218 1110 10 0.4367959399812804 1110 11 0.6591006798277093 1110 12 0.7693148417003783 1110 13 0.5625727283534019 1110 14 0.5042570816389897 1110 15 0.5367245168416304 1110 16 0.6788485737451211 1110 17 0.38498387811920765 1110 18 0.7794792657291225 1110 19 0.5187375070613598 1110 20 0.7161897544606006 1110 21 0.5399771336178745 1110 22 0.7742363385208435 1110 23 0.6536846481473404 1110 24 0.6309906558254781 1110 25 0.5667273424135502 1111 1 0.3077052520105106 1111 2 0.1908351400267101 1111 3 0.1366863095293489 1111 4 0.10745323359367376 1111 5 0.10239138815901327 1111 6 0.12241251651679619 1111 7 0.16686249006990295 1111 8 -0.07448213963470351 1111 9 -0.15657268287910853 1111 10 0.05083048841069626 1111 11 0.21129289685992347 1111 12 0.1521266976549711 1111 13 -0.04879865957798302 1111 14 -0.023780856521476323 1111 15 6.973146638244998E-5 1111 16 0.04897199293021259 1111 17 -0.05316191384967084 1111 18 -0.06122712851085055 1111 19 -0.02229626309922139 1111 20 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2215 17 0.28720035532712984 2215 18 0.28189212803537345 2215 19 0.3589025859103714 2215 20 0.4113090997037645 2215 21 0.29983276828390615 2215 22 0.624860431485196 2215 23 0.46661538765915367 2215 24 0.5588053668784951 2215 25 0.6468506271575194 2216 1 -0.31704816558178095 2216 2 -0.10850526864873444 2216 3 -1.5548118480813393 2216 4 -1.4705731500593089 2216 5 -0.3453251656956482 2216 6 -0.08260845960392516 2216 7 -0.05570638019717933 2216 8 0.07139760790744276 2216 9 2.1650843693521438E-4 2216 10 -0.11384136424069169 2216 11 -0.09210183172662312 2216 12 0.006106045879846533 2216 13 0.013600647066844829 2216 14 -0.06435064133678026 2216 15 -0.12173799008558461 2216 16 0.14060494006188407 2216 17 0.07140141595662897 2216 18 -0.017576060563108342 2216 19 0.05278176594300277 2216 20 -0.3076627482161314 2216 21 -0.0620534160940025 2216 22 0.07497317949339788 2216 23 0.06301742909654706 2216 24 -0.04447380417420277 2216 25 0.047666054677016234 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 12986.0 3 15.0 4 229.0 5 1384.0 6 1863.0 7 1357.0 8 1071.0 9 965.0 10 870.0 11 725.0 12 686.0 13 647.0 14 637.0 15 714.0 16 720.0 17 767.0 18 813.0 19 1029.0 20 1309.0 21 1643.0 22 2038.0 23 2619.0 24 3037.0 25 3670.0 26 4227.0 27 4643.0 28 5590.0 29 6838.0 30 8722.0 31 11085.0 32 14777.0 33 19442.0 34 26356.0 35 36523.0 36 53971.0 37 92595.0 38 211475.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.35811198023266 19.76641634973914 10.72114346955635 28.154328200471856 2 13.816802982830861 21.082057617456407 40.75809896627943 24.34304043343331 3 18.502662862135306 25.28780288314078 29.324199979552194 26.88533427517171 4 12.188544303562201 17.77904162902992 37.99452826752014 32.03788579988774 5 12.682127144681301 38.94988448320406 35.4414451587961 12.926543213318539 6 31.354022902756533 38.188854489164086 17.794770412921036 12.662352195158341 7 27.733245094175473 31.904961965452884 21.875627402720045 18.4861655376516 8 24.50686632415593 38.96573901276608 19.837431318170665 16.689963344907323 9 26.08622916995827 15.914198351255147 20.133832727678282 37.8657397511083 10 15.091186879200619 30.041299886992206 33.81163385475524 21.055879379051923 11 33.85320538198607 22.79500461881316 24.84791372763314 18.503876271567627 12 23.50233069906103 25.09293058457055 31.852701306232294 19.552037410136126 13 29.11061382737894 22.71677998044733 25.668266317742 22.50433987443173 14 20.411011861615723 23.39054118854059 27.25513811292139 28.9433088369223 15 22.33419699712649 32.49761160407272 23.15071242969566 22.017478969105124 16 21.132532762860272 28.78466562459924 28.946551605570665 21.136250006969835 17 20.8416505897353 29.70905400733777 28.220497436984004 21.228797965942924 18 20.795224120274256 28.05457617227068 30.684320966773658 20.465878740681404 19 23.26279888854814 27.137268021597112 27.519585157099446 22.080347932755302 20 23.14366165769885 28.114713726802158 28.286263904764557 20.455360710734432 21 22.14171261167146 27.145597644443285 27.43520917834345 23.277480565541804 22 22.668101509558806 29.041071448485052 27.208858853835604 21.08196818812054 23 21.477930844776186 28.787664765926447 28.032139113367876 21.70226527592949 24 22.78909668090358 27.402711332656803 28.80261245488237 21.00557953155725 25 22.140892193308552 28.028996282527878 28.434572490706323 21.39553903345725 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 222.0 1 222.0 2 223.0 3 224.0 4 224.0 5 224.0 6 375.5 7 527.0 8 527.0 9 527.0 10 836.0 11 1145.0 12 1145.0 13 1145.0 14 1802.5 15 2460.0 16 2460.0 17 2460.0 18 4338.0 19 6216.0 20 6216.0 21 6216.0 22 9903.0 23 13590.0 24 13590.0 25 13590.0 26 20018.5 27 26447.0 28 26447.0 29 26447.0 30 35409.0 31 44371.0 32 44371.0 33 44371.0 34 49620.0 35 54869.0 36 54869.0 37 54869.0 38 58394.5 39 61920.0 40 61920.0 41 61920.0 42 64874.5 43 67829.0 44 67829.0 45 67829.0 46 70179.5 47 72530.0 48 72530.0 49 72530.0 50 68644.5 51 64759.0 52 64759.0 53 64759.0 54 56486.5 55 48214.0 56 48214.0 57 48214.0 58 40734.0 59 33254.0 60 33254.0 61 33254.0 62 26886.5 63 20519.0 64 20519.0 65 20519.0 66 15813.0 67 11107.0 68 11107.0 69 11107.0 70 8026.0 71 4945.0 72 4945.0 73 4945.0 74 3434.5 75 1924.0 76 1924.0 77 1924.0 78 1308.5 79 693.0 80 693.0 81 693.0 82 442.0 83 191.0 84 191.0 85 191.0 86 126.0 87 61.0 88 61.0 89 61.0 90 38.5 91 16.0 92 16.0 93 16.0 94 10.5 95 5.0 96 5.0 97 5.0 98 2.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.10891424025812303 2 0.10445358877997465 3 0.01542641969526316 4 0.0 5 0.0039030700433798356 6 0.34532876859998735 7 0.01970121069515536 8 0.009293023912809133 9 0.00985060534757768 10 0.004088930521636018 11 0.0039030700433798356 12 3.7172095651236527E-4 13 7.434419130247305E-4 14 0.0 15 0.0037172095651236527 16 9.293023912809132E-4 17 0.0 18 1.8586047825618263E-4 19 5.57581434768548E-4 20 5.57581434768548E-4 21 0.014125396347469883 22 0.0 23 3.7172095651236527E-4 24 0.0 25 0.007062698173734941 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 538038.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 81.42677045527331 #Duplication Level Percentage of deduplicated Percentage of total 1 92.13790828489843 75.0249230814345 2 5.829717006551814 9.493900570233953 3 0.9594956203508929 2.343858888934567 4 0.3751988008296539 1.2220490652100016 5 0.18176629442243974 0.7400321166220813 6 0.10952663248495556 0.5351039977254936 7 0.07971190453263853 0.4543478067052273 8 0.04980778863728734 0.32445498978025333 9 0.03944223713327696 0.289048859036442 >10 0.19539931860984688 3.0192415651529134 >50 0.021926674363270025 1.3054009010708834 >100 0.01735860484204853 3.0199584778560467 >500 0.0015988188670251378 0.9747260981904584 >1k 0.0011420134764465269 1.252953582047151 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 1477 0.27451592638438177 No Hit GTATCAACGCAGAGTACTTTTTTTT 1444 0.26838253060192774 No Hit CTGTAGGACGTGGAATATGGCAAGA 1437 0.26708150725413443 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1207 0.22433359725521246 No Hit CTTTAGGACGTGAAATATGGCGAGG 1172 0.21782848051624606 No Hit GGTATCAACGCAGAGTACTTTTTTT 932 0.17322196573476223 No Hit GTCCTACAGTGTGCATTTCTCATTT 890 0.16541582564800256 No Hit TATCAACGCAGAGTACTTTTTTTTT 787 0.14627219638761574 No Hit CTGTAGGACCTGGAATATGGCGAGA 725 0.13474884673573242 No Hit GTACATGGGAAGCAGTGGTATCAAC 706 0.13121749764886495 No Hit CTGAAGGACCTGGAATATGGCGAGA 602 0.11188800791022196 No Hit CCCATGTACTCTGCGTTGATACCAC 599 0.11133042647545341 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.8586047825618266E-4 2 0.0 0.0 0.0 0.0 1.8586047825618266E-4 3 0.0 0.0 0.0 0.0 1.8586047825618266E-4 4 0.0 0.0 0.0 0.0 1.8586047825618266E-4 5 0.0 0.0 0.0 0.0 1.8586047825618266E-4 6 0.0 0.0 0.0 0.0 1.8586047825618266E-4 7 0.0 0.0 0.0 0.0 1.8586047825618266E-4 8 0.0 0.0 0.0 0.0 1.8586047825618266E-4 9 0.0 0.0 0.0 1.8586047825618266E-4 1.8586047825618266E-4 10 0.0 0.0 0.0 1.8586047825618266E-4 1.8586047825618266E-4 11 0.0 0.0 0.0 1.8586047825618266E-4 1.8586047825618266E-4 12 0.0 0.0 0.0 1.8586047825618266E-4 1.8586047825618266E-4 13 0.0 0.0 0.0 1.8586047825618266E-4 1.8586047825618266E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCAACGA 40 2.7805803E-4 16.606375 19 TAGGACC 320 0.0 14.875513 4 TCCAACG 80 1.3007775E-7 14.234036 18 ATAGGAC 55 1.9212326E-4 13.847751 3 AGGACCC 50 0.0014733888 13.32846 5 TAAGACT 100 9.71886E-9 13.32846 4 GACGTGG 410 0.0 12.731993 7 GTTTAGG 60 3.9892315E-4 12.702063 1 ACTACAC 60 4.011276E-4 12.693771 3 CTAAGAC 75 1.442264E-5 12.69377 3 AGGACCT 520 0.0 12.632743 5 GGACCTG 505 0.0 12.63093 6 CTGTAGG 845 0.0 12.514161 1 GTAGGAC 865 0.0 12.43696 3 GGACGTG 800 0.0 12.376427 6 TGTAGGA 920 0.0 12.322381 2 TCCTATA 55 0.0030005642 12.124697 2 CGTCAAG 55 0.00301528 12.116781 3 AGGACGT 825 0.0 12.001384 5 TAGGACG 825 0.0 12.001384 4 >>END_MODULE