FastQCFastQC Report
Thu 26 May 2016
SRR1926903_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926903_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences310881
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT5000.16083324487504866No Hit
GTCCTACAGTGTGCATTTCTCATTT3400.10936660651503308No Hit
GTCCTACAGTGGACATTTCTAAATT3320.1067932745970323No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCTATA453.4170916E-516.949722
GTCCTAT400.00514230614.3013281
TAGAGCT400.005186649514.2828414
GGTATCA1700.014.0209091
TCCTGGG852.560846E-713.46007252
TAGGACC852.6015368E-713.4426754
TGGAGTG500.001472694813.3263455
GTGTAGC853.7602767E-612.33841
GTCCTAC2100.011.350261
AGGACCT1604.3655746E-1111.3035965
GACGTGA855.185187E-511.1986097
GGACGTG1451.1586963E-911.1599926
ATCCTGG600.005702926311.1232541
GTGTTAG600.005702926311.1232541
CCTATAG600.005702926311.1232543
TGAACAG600.005772012311.1052885
AGGCTGA600.005772012311.1052886
GTCCACT600.005940746511.06240311
GTGCAGA951.2975799E-511.0396211
GTCCTAA951.2975799E-511.0396211
Produced by FastQC (version 0.11.5)