##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926903_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 310881 Sequences flagged as poor quality 0 Sequence length 25 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.797327594803157 31.0 28.0 31.0 16.0 34.0 2 27.77737140577906 31.0 28.0 31.0 16.0 34.0 3 27.719863870741538 31.0 28.0 31.0 12.0 34.0 4 31.34904030802783 35.0 32.0 37.0 17.0 37.0 5 31.099501095274398 35.0 32.0 37.0 16.0 37.0 6 30.56026582518713 35.0 30.0 37.0 11.0 37.0 7 30.647521077196743 35.0 31.0 37.0 10.0 37.0 8 30.40065812963803 35.0 30.0 37.0 10.0 37.0 9 32.61350162924077 37.0 32.0 39.0 15.0 39.0 10 32.80181805900007 37.0 33.0 39.0 15.0 39.0 11 32.97685287939758 37.0 33.0 39.0 15.0 39.0 12 32.819207349435956 37.0 33.0 39.0 10.0 39.0 13 32.93814031735616 38.0 33.0 39.0 11.0 39.0 14 33.94154354881771 39.0 33.0 41.0 11.0 41.0 15 33.478263386955135 39.0 33.0 40.0 10.0 41.0 16 33.56972925331558 39.0 33.0 40.0 10.0 41.0 17 33.80668487298998 39.0 33.0 41.0 10.0 41.0 18 33.85580656263972 39.0 33.0 41.0 10.0 41.0 19 33.24474959872106 39.0 32.0 40.0 7.0 41.0 20 33.48797449828069 39.0 33.0 41.0 2.0 41.0 21 33.4741910891949 39.0 33.0 41.0 2.0 41.0 22 33.494938577783785 39.0 33.0 41.0 2.0 41.0 23 33.49515731099681 39.0 34.0 41.0 2.0 41.0 24 32.185775264490275 38.0 31.0 40.0 2.0 41.0 25 32.74840855504196 39.0 33.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.2749374531355002 1101 2 -0.03747757211949221 1101 3 -0.39477042810245777 1101 4 0.1000412317003061 1101 5 -0.3667991626441136 1101 6 -2.7260217055650564 1101 7 -0.5245039317088747 1101 8 -0.14664784346981463 1101 9 0.2897097233629964 1101 10 -8.875100875257303 1101 11 -3.604228659788607 1101 12 -1.1385090111142588 1101 13 -0.6809282719390666 1101 14 0.1861845377287139 1101 15 -1.4013650657450114 1101 16 0.16272968737349913 1101 17 0.36979225582182806 1101 18 0.43942963787107914 1101 19 -0.8301546797703026 1101 20 0.10950953802500152 1101 21 0.297438081352027 1101 22 0.5382578091931691 1101 23 0.5413115321686064 1101 24 0.7279897924935668 1101 25 0.6683458517473468 1102 1 0.3288313852903002 1102 2 0.3379165989268529 1102 3 0.10167008790327614 1102 4 0.19545451407011072 1102 5 0.4302146930653947 1102 6 -0.05077086944465492 1102 7 0.7301544533842907 1102 8 1.0590855535679324 1102 9 0.8606127333964366 1102 10 0.7619989814077712 1102 11 0.6641420998864973 1102 12 0.5334929000180892 1102 13 0.6635533334121035 1102 14 0.2767244326164331 1102 15 0.7454098984212578 1102 16 0.6091463142249083 1102 17 0.2647277551051559 1102 18 0.04573637461259494 1102 19 0.43190934316328367 1102 20 0.37362802823044916 1102 21 0.4295928830720399 1102 22 0.18527166490267177 1102 23 -0.0028356250220298307 1102 24 -3.4200990751605147 1102 25 -1.4458681950753949 1103 1 0.19266468223618105 1103 2 -0.1956981307143657 1103 3 0.6000035425281212 1103 4 0.5335473263351069 1103 5 -0.10168254876562344 1103 6 0.4327747240347115 1103 7 0.37001569589454064 1103 8 0.5956306861395539 1103 9 0.818772013410026 1103 10 1.003116803982543 1103 11 0.4994185232423902 1103 12 0.2263148683367433 1103 13 0.2869189302876336 1103 14 0.20091723760393876 1103 15 0.8019365946830561 1103 16 0.7317526262351919 1103 17 0.4132225274409862 1103 18 0.4937726650329921 1103 19 -1.4126386033714198 1103 20 0.02660330902030239 1103 21 0.1159427363093144 1103 22 0.5195995886345699 1103 23 0.38585515439722684 1103 24 2.3316705679998577 1103 25 1.2640316181356752 1104 1 0.41189397487463 1104 2 0.1777398191848576 1104 3 -0.9621617324502836 1104 4 0.16960644909161005 1104 5 0.2505921417037129 1104 6 -1.1434130099128836 1104 7 0.11462650307373323 1104 8 0.4555260695736649 1104 9 0.7353618972760358 1104 10 0.6901165160470413 1104 11 0.8022930793418297 1104 12 0.7049692497553082 1104 13 0.6560282498000589 1104 14 0.2796627985982809 1104 15 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1.0114845806577968 2215 5 1.2265013060806282 2215 6 1.7328436572925447 2215 7 1.071099753437398 2215 8 -0.3810263791090591 2215 9 0.45277744600724645 2215 10 0.10234376673396639 2215 11 -0.163514258624879 2215 12 -0.4167052289904518 2215 13 0.07347612437578732 2215 14 0.06043700257436058 2215 15 -0.00955966732897906 2215 16 0.17524787066103187 2215 17 -0.12956122728791541 2215 18 -0.835970394454769 2215 19 0.43577388011332374 2215 20 -3.7483213978081977 2215 21 -2.1885001762849576 2215 22 -1.2240179302605299 2215 23 -1.1230815501006717 2215 24 -1.1327262249623864 2215 25 -0.9537892702827158 2216 1 -0.5926612563436819 2216 2 -1.2736121550047272 2216 3 -0.6931188687231362 2216 4 -0.9055356618317276 2216 5 -0.4908197495238191 2216 6 -1.6780880752070928 2216 7 -9.170148423950387 2216 8 -10.940118422667847 2216 9 -5.7896726702458565 2216 10 -3.6833433088108443 2216 11 -2.739748708951307 2216 12 -2.42248504429908 2216 13 -1.859143051475094 2216 14 -2.0865148016475352 2216 15 -2.9925157801404616 2216 16 -2.081678055751407 2216 17 -2.3887728079360464 2216 18 -2.0563915328156632 2216 19 -1.792115042345202 2216 20 -2.7620221872784256 2216 21 -5.327235447607876 2216 22 -3.748483625743077 2216 23 -3.728576315427034 2216 24 -3.82489851600236 2216 25 -3.4254550393265504 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 20812.0 3 2.0 4 161.0 5 999.0 6 1197.0 7 1024.0 8 735.0 9 713.0 10 671.0 11 618.0 12 628.0 13 580.0 14 580.0 15 617.0 16 677.0 17 731.0 18 838.0 19 1048.0 20 1287.0 21 1751.0 22 2157.0 23 2548.0 24 2992.0 25 3546.0 26 4007.0 27 4221.0 28 4599.0 29 5606.0 30 6676.0 31 8122.0 32 10254.0 33 13191.0 34 17769.0 35 24798.0 36 36011.0 37 56460.0 38 72255.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.84387189952094 17.453271178681728 12.096945782316872 25.605911139480455 2 15.236368087409163 18.6221202906767 41.91523218059063 24.226279441323506 3 19.969977708770887 22.65072349856331 28.76663788993545 28.61266090273035 4 11.744331277442283 15.573696612286417 37.87485675289391 34.80711535737739 5 13.549478940442523 37.42748762101484 35.54884189325277 13.474191545289873 6 33.464495994337376 36.13139860364853 17.266175476979505 13.137929925034586 7 29.33019665550113 30.456754506554105 21.283262661271067 18.9297861766737 8 25.314646508641687 37.351387800007096 20.034457059341637 17.299508632009577 9 25.908397732464238 15.814195906338677 19.193943800631875 39.083462560565216 10 14.518495636728703 29.369706798465778 34.07759929981723 22.034198264988287 11 35.99073448508831 22.379435704404337 22.631341891065855 18.998487919441494 12 23.023244591007803 24.554009490871067 31.206305799083083 21.216440119038044 13 29.3848163328336 21.249284183840892 25.192546503921708 24.1733529794038 14 20.73262147354333 21.531774705706354 27.045095534843917 30.690508285906397 15 23.700600959991764 30.158668880052502 22.57878752782818 23.561942632127554 16 22.606040717576505 28.359457442153758 26.669755231256275 22.364746609013462 17 22.26790724011016 28.254253210820274 26.901075849998712 22.576763699070856 18 21.861829786960243 27.89688386727321 28.330234144269934 21.91105220149662 19 23.927619979150847 26.812074490675798 26.811109252133175 22.44919627804018 20 23.359467745931042 27.260326417419222 27.72585569557538 21.654350141074353 21 23.419177377065004 26.680071420528577 26.748918236363345 23.151832966043077 22 23.066999115257783 27.37295906056463 27.212418563500364 22.34762326067723 23 22.816228963378343 27.07905040424416 27.132778040517714 22.971942591859783 24 23.24713512678793 26.826215922325098 27.55940469832773 22.367244252559242 25 22.81088941364201 26.79404440955623 27.577420745478292 22.81764543132347 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 83.0 1 83.0 2 107.5 3 132.0 4 132.0 5 132.0 6 210.5 7 289.0 8 289.0 9 289.0 10 422.5 11 556.0 12 556.0 13 556.0 14 927.5 15 1299.0 16 1299.0 17 1299.0 18 2200.5 19 3102.0 20 3102.0 21 3102.0 22 4865.5 23 6629.0 24 6629.0 25 6629.0 26 9441.0 27 12253.0 28 12253.0 29 12253.0 30 15937.0 31 19621.0 32 19621.0 33 19621.0 34 22998.0 35 26375.0 36 26375.0 37 26375.0 38 29329.0 39 32283.0 40 32283.0 41 32283.0 42 35081.0 43 37879.0 44 37879.0 45 37879.0 46 39773.5 47 41668.0 48 41668.0 49 41668.0 50 41121.5 51 40575.0 52 40575.0 53 40575.0 54 36848.0 55 33121.0 56 33121.0 57 33121.0 58 28554.5 59 23988.0 60 23988.0 61 23988.0 62 19877.5 63 15767.0 64 15767.0 65 15767.0 66 12252.0 67 8737.0 68 8737.0 69 8737.0 70 6396.5 71 4056.0 72 4056.0 73 4056.0 74 2853.0 75 1650.0 76 1650.0 77 1650.0 78 1112.0 79 574.0 80 574.0 81 574.0 82 375.5 83 177.0 84 177.0 85 177.0 86 108.5 87 40.0 88 40.0 89 40.0 90 29.0 91 18.0 92 18.0 93 18.0 94 12.5 95 7.0 96 7.0 97 7.0 98 4.5 99 2.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.22098487845831682 2 0.13928159006179214 3 0.1431415879387933 4 0.07366162615277227 5 0.023159987262007006 6 0.02283832077225691 7 0.3213448232603472 8 0.2991498354675905 9 0.01897832289525574 10 0.03377498142376022 11 0.016404990977254962 12 0.018013323426005447 13 0.015118325018254573 14 0.01672665746700506 15 0.01447499203875438 16 0.018334989915755544 17 0.018334989915755544 18 0.015118325018254573 19 0.02476831971075749 20 0.01672665746700506 21 0.014796658528504477 22 0.018013323426005447 23 0.017369990446505255 24 0.015118325018254573 25 0.015118325018254573 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 310881.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 90.56511565613391 #Duplication Level Percentage of deduplicated Percentage of total 1 95.02573230507637 86.06016436518061 2 3.808910907761878 6.89908913770729 3 0.5769774538432273 1.5676208951488062 4 0.22887952003509174 0.8291400081319402 5 0.10746596874730027 0.48663339443488607 6 0.06847142427731678 0.37206734752892456 7 0.03604302368679304 0.22849684261538297 8 0.028727530330447032 0.2081369685513624 9 0.01709583817195899 0.13934579051538132 >10 0.08642016368010724 1.403085057900989 >50 0.008881316505750164 0.5511320252015754 >100 0.0063945478837624 1.2550881670827838 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 500 0.16083324487504866 No Hit GTCCTACAGTGTGCATTTCTCATTT 340 0.10936660651503308 No Hit GTCCTACAGTGGACATTTCTAAATT 332 0.1067932745970323 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCCTATA 45 3.4170916E-5 16.94972 2 GTCCTAT 40 0.005142306 14.301328 1 TAGAGCT 40 0.0051866495 14.282841 4 GGTATCA 170 0.0 14.020909 1 TCCTGGG 85 2.560846E-7 13.4600725 2 TAGGACC 85 2.6015368E-7 13.442675 4 TGGAGTG 50 0.0014726948 13.326345 5 GTGTAGC 85 3.7602767E-6 12.3384 1 GTCCTAC 210 0.0 11.35026 1 AGGACCT 160 4.3655746E-11 11.303596 5 GACGTGA 85 5.185187E-5 11.198609 7 GGACGTG 145 1.1586963E-9 11.159992 6 ATCCTGG 60 0.0057029263 11.123254 1 GTGTTAG 60 0.0057029263 11.123254 1 CCTATAG 60 0.0057029263 11.123254 3 TGAACAG 60 0.0057720123 11.105288 5 AGGCTGA 60 0.0057720123 11.105288 6 GTCCACT 60 0.0059407465 11.062403 11 GTGCAGA 95 1.2975799E-5 11.039621 1 GTCCTAA 95 1.2975799E-5 11.039621 1 >>END_MODULE