FastQCFastQC Report
Thu 26 May 2016
SRR1926903_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926903_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences310881
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT7720.24832653008707511No Hit
GGTATCAACGCAGAGTACTTTTTTT5560.1788465683010541No Hit
TATCAACGCAGAGTACTTTTTTTTT4420.142176588469543No Hit
GTCCTACAGTGTGCATTTCTCATTT3290.105828275127782No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC1250.017.5147384
GGCGAGG504.57838E-617.0843619
GTTTATG400.005180896314.2852351
TGCTACA400.005180896314.2852352
AGGACCT1800.014.2783195
TTACTCT400.00519757614.2783194
CTGTGCG400.005304265314.2346769
ACTGTTT400.00530993114.2323878
GTAGGAC1650.013.8456433
TAAACTG500.001472628313.3264315
TGCACCT500.001472628313.3264315
TGGCGAG1850.012.82609718
GGACCTG2000.012.3745446
ATAGGAC550.003013773412.1149383
GTTACAC550.003013773412.1149383
CGTGAAA951.0443855E-611.9870979
ACTGTGC951.0464191E-611.9851688
ATGGCGA2000.011.8641417
GTCCTAC2100.011.7909881
TTTAGGA1156.756272E-811.5938142
Produced by FastQC (version 0.11.5)