FastQCFastQC Report
Thu 26 May 2016
SRR1926902_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926902_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences646985
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT11910.18408463874742073No Hit
GTCCTAAAGTGTGTATTTCTCATTT10220.15796347674211922No Hit
CTGTAGGACGTGGAATATGGCAAGA9750.1506990115690472No Hit
GTATCAACGCAGAGTACTTTTTTTT9530.14729862361569432No Hit
GTCCTACAGTGTGCATTTCTCATTT8510.13153318855924015No Hit
CTTTAGGACGTGAAATATGGCGAGG7940.12272309249828049No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTAGAC655.273278E-513.1982823
TTAGACT655.3196145E-513.18598654
ATGTCGA655.54066E-513.12890817
TGTCGAG655.54066E-513.12890818
CTCTTAC603.9404692E-412.7213191
TCCAACG604.1522217E-412.642652518
CCCTTAT701.0460793E-412.2669861
CTTAGAC550.002988634412.1317543
GTCCTAA3100.012.003181
TTAGGAC3600.011.9151153
TAGGACC2400.011.9040164
ACAATAG1052.594079E-711.8016383
ATAAGAC657.7886984E-411.7318063
AGGACGT5600.011.5585175
AAATGTC1900.011.5227337
CTGTAGG7150.011.3424351
GTATGGG855.08346E-511.2246921
TAAGACT855.1810057E-511.2037794
GATATAC2050.011.169941
GACGTGA3000.011.1052427
Produced by FastQC (version 0.11.5)