##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926902_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 646985 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.980950099306785 31.0 28.0 31.0 16.0 34.0 2 27.98077853427823 31.0 28.0 31.0 16.0 34.0 3 27.925398579565215 31.0 28.0 33.0 16.0 34.0 4 31.572521774075135 35.0 32.0 37.0 18.0 37.0 5 31.247280848860484 35.0 32.0 37.0 17.0 37.0 6 30.73623036082753 35.0 31.0 37.0 11.0 37.0 7 30.877470111362705 35.0 32.0 37.0 11.0 37.0 8 30.607281467112838 35.0 31.0 37.0 10.0 37.0 9 32.81755527562463 37.0 32.0 39.0 17.0 39.0 10 33.002142244410614 37.0 33.0 39.0 17.0 39.0 11 33.17862083355874 38.0 33.0 39.0 17.0 39.0 12 33.054262463581075 38.0 33.0 39.0 16.0 39.0 13 33.161618893791974 38.0 33.0 39.0 17.0 39.0 14 34.154541449956334 39.0 34.0 41.0 17.0 41.0 15 33.71854216094655 39.0 33.0 41.0 10.0 41.0 16 33.800177747552105 39.0 33.0 40.0 11.0 41.0 17 34.02519996599612 39.0 34.0 41.0 13.0 41.0 18 34.07298932741872 39.0 34.0 41.0 11.0 41.0 19 33.46176031901822 39.0 33.0 40.0 10.0 41.0 20 33.73350232231041 39.0 33.0 41.0 10.0 41.0 21 33.72824717729159 39.0 34.0 41.0 2.0 41.0 22 33.74473905886535 39.0 34.0 41.0 2.0 41.0 23 33.72880978693478 39.0 34.0 41.0 2.0 41.0 24 32.42814748409932 38.0 32.0 40.0 2.0 41.0 25 33.01175143164061 39.0 33.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.3894751377083523 1101 2 -0.6348048986691204 1101 3 -1.0504696302449972 1101 4 -0.5351824849783 1101 5 -0.6739153426516538 1101 6 -2.3717454763801875 1101 7 -0.7845435332180699 1101 8 -0.22990130245175067 1101 9 -0.5575123943420763 1101 10 -8.932892941927928 1101 11 -3.9858015342653736 1101 12 -1.7680535140469686 1101 13 -1.0538217422664289 1101 14 -0.6412281422959438 1101 15 -1.9994761930918052 1101 16 -0.5761024737146059 1101 17 -0.5378420076907418 1101 18 -0.5868886340369102 1101 19 -1.0847693695318483 1101 20 -0.406928810366864 1101 21 -0.3707252677527606 1101 22 -0.09487020864634843 1101 23 -0.4239778660301283 1101 24 -0.45993923294363626 1101 25 -0.1637000174468639 1102 1 -0.004432098555721353 1102 2 -0.0015637106334089879 1102 3 -0.09820499989817577 1102 4 -0.03153430667258661 1102 5 -0.20175280199596202 1102 6 -0.99308874498373 1102 7 -0.05498884133460891 1102 8 0.06678944971845269 1102 9 0.39387982074876504 1102 10 0.5491375846231463 1102 11 0.07828506406779923 1102 12 0.5618711275215063 1102 13 0.16012641003788985 1102 14 -3.7698432149824157E-4 1102 15 0.42236794450575843 1102 16 0.4511613583437466 1102 17 0.5602311520262546 1102 18 0.2143626261184579 1102 19 0.7918665516246222 1102 20 0.5794968644149705 1102 21 0.4406049545363686 1102 22 0.49734009246806465 1102 23 0.20117437898512947 1102 24 -2.70803132350148 1102 25 -1.080256064139899 1103 1 -0.08257562344636327 1103 2 -0.4401127446703761 1103 3 0.13036688292344323 1103 4 -0.07597928107558616 1103 5 -0.5776766333237795 1103 6 0.2142140082266799 1103 7 0.045994383205801626 1103 8 0.2080601237803137 1103 9 0.2471740431405749 1103 10 0.36907621542221847 1103 11 0.05739409774136561 1103 12 -0.10515326117129575 1103 13 0.11764720334042522 1103 14 -0.010864485627465115 1103 15 0.5145160028133162 1103 16 0.4111475945629337 1103 17 0.22587348451013867 1103 18 0.03311452829113648 1103 19 -1.5304796051916298 1103 20 -0.13464462543480948 1103 21 0.12905746040755162 1103 22 0.03865301179776992 1103 23 0.08034269230874003 1103 24 1.588369197977741 1103 25 0.7675315103003513 1104 1 -0.39876503834320687 1104 2 -0.38679590268378305 1104 3 -1.5963356571197416 1104 4 -0.4709282460665243 1104 5 -0.6510345808231932 1104 6 -1.4714437233387088 1104 7 -0.43057916010674546 1104 8 -0.02402125472861627 1104 9 0.3139900135862277 1104 10 0.26952325955427625 1104 11 0.19502060125788034 1104 12 0.22165074184658096 1104 13 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-0.1040923492631336 2215 3 -0.004076505047660106 2215 4 0.13351875097974997 2215 5 0.6186755662663437 2215 6 1.1861703232645056 2215 7 0.6380464627115252 2215 8 -0.7068911416516706 2215 9 -0.34548129947877726 2215 10 -0.5049866906202638 2215 11 -0.39390069241398606 2215 12 -0.7174473277897278 2215 13 -0.2970133214700752 2215 14 -0.2982421756884932 2215 15 -0.16538425515980038 2215 16 0.04507105875164541 2215 17 -0.4039256298200584 2215 18 -0.9532651732782895 2215 19 0.09119297730483567 2215 20 -3.9702398008451567 2215 21 -2.413013749262845 2215 22 -1.5131073421670962 2215 23 -1.3255615204944533 2215 24 -1.3647154937235548 2215 25 -1.0261278818528723 2216 1 -0.34883359496268795 2216 2 -0.8052333014497641 2216 3 -0.0782943423393867 2216 4 -0.3437839682841428 2216 5 0.0366344564459915 2216 6 -1.4214105750831365 2216 7 -9.607152948877022 2216 8 -10.453276097010722 2216 9 -5.6896468774087445 2216 10 -3.3424330902200907 2216 11 -2.1953537856877325 2216 12 -1.8332077377032086 2216 13 -1.875912452345549 2216 14 -2.0001589556166905 2216 15 -2.5529139143221364 2216 16 -1.718832959098151 2216 17 -2.167402654458968 2216 18 -1.966167114575363 2216 19 -1.4851236262315552 2216 20 -2.488959216119504 2216 21 -4.88955956341302 2216 22 -3.6817778876982317 2216 23 -3.511628542796828 2216 24 -3.527939613327728 2216 25 -2.8581805097945185 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 40294.0 3 3.0 4 355.0 5 2060.0 6 2469.0 7 1870.0 8 1601.0 9 1393.0 10 1326.0 11 1278.0 12 1317.0 13 1257.0 14 1288.0 15 1274.0 16 1418.0 17 1424.0 18 1720.0 19 2197.0 20 2657.0 21 3449.0 22 4359.0 23 5259.0 24 5960.0 25 6863.0 26 8027.0 27 8742.0 28 9643.0 29 11246.0 30 13441.0 31 16649.0 32 21196.0 33 27825.0 34 37555.0 35 52273.0 36 75345.0 37 120258.0 38 151694.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.54236421685646 19.208313130389925 12.720960818424466 25.52836183432915 2 14.009196049815289 20.340108365433874 42.78577475404204 22.8649208307088 3 18.499934997802306 25.238031857267558 30.237319928435674 26.02471321649446 4 11.290237760853291 17.117327525938393 40.26297228907463 31.329462424133688 5 12.65262519981572 38.4145530775486 36.624791681482144 12.308030041153534 6 30.282520812237674 38.16836849062 18.99158234843976 12.557528348702569 7 26.713296036038546 31.662085889190138 23.896776713838598 17.72784136093271 8 23.73623068886085 37.78302076906621 22.27117288333173 16.20957565874121 9 25.914944270100637 15.635444525175075 20.82365854035587 37.62595266436842 10 13.952190335712839 29.868439158715653 35.66707330083352 20.512297204737983 11 33.60746334412849 23.00914367864955 25.22356796698073 18.159825010241228 12 22.417122054151818 25.414409052613678 32.86905709670189 19.299411796532613 13 28.58406026288493 22.716838331795223 25.975770560783058 22.723330844536783 14 20.049746013980823 22.96880748694515 28.424482830050422 28.5569636690236 15 22.533536971825544 31.887716456071903 23.53060296986542 22.04814360223714 16 21.111684810334005 28.736353602092525 29.081861382587643 21.070100204985824 17 20.821423547289516 29.311136429336642 28.57251069368674 21.294929329687097 18 20.643776027899126 28.508491239785933 30.47617722622592 20.37155550608902 19 23.220306753303372 27.469586713231575 27.765487238710097 21.544619294754956 20 22.729767206743723 28.315630492685695 28.693906007732465 20.26069629283812 21 22.391663587866383 27.518136581248637 27.574868951316823 22.515330879568154 22 22.098604547625783 28.939675708431302 28.07198165985713 20.88973808408578 23 21.624600582179976 28.510586987674664 28.242066908958247 21.622745521187113 24 22.728488328054254 27.667172159847148 28.68897947574019 20.915360036358408 25 22.033251145858294 27.965434885105 28.65550050626454 21.345813462772163 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 207.0 1 207.0 2 276.5 3 346.0 4 346.0 5 346.0 6 526.0 7 706.0 8 706.0 9 706.0 10 1142.0 11 1578.0 12 1578.0 13 1578.0 14 2680.0 15 3782.0 16 3782.0 17 3782.0 18 6504.0 19 9226.0 20 9226.0 21 9226.0 22 14215.5 23 19205.0 24 19205.0 25 19205.0 26 27135.0 27 35065.0 28 35065.0 29 35065.0 30 44976.0 31 54887.0 32 54887.0 33 54887.0 34 61797.5 35 68708.0 36 68708.0 37 68708.0 38 73177.0 39 77646.0 40 77646.0 41 77646.0 42 80405.0 43 83164.0 44 83164.0 45 83164.0 46 83474.0 47 83784.0 48 83784.0 49 83784.0 50 78950.0 51 74116.0 52 74116.0 53 74116.0 54 64279.0 55 54442.0 56 54442.0 57 54442.0 58 45819.5 59 37197.0 60 37197.0 61 37197.0 62 29870.0 63 22543.0 64 22543.0 65 22543.0 66 17171.0 67 11799.0 68 11799.0 69 11799.0 70 8554.5 71 5310.0 72 5310.0 73 5310.0 74 3750.0 75 2190.0 76 2190.0 77 2190.0 78 1468.0 79 746.0 80 746.0 81 746.0 82 489.0 83 232.0 84 232.0 85 232.0 86 156.5 87 81.0 88 81.0 89 81.0 90 49.5 91 18.0 92 18.0 93 18.0 94 10.5 95 3.0 96 3.0 97 3.0 98 3.5 99 4.0 100 4.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2244256049212887 2 0.1295238684049862 3 0.1318423147368177 4 0.07001707922131116 5 0.021484269341638523 6 0.020093201542539626 7 0.2933607425210785 8 0.29227880089955716 9 0.01777475521070813 10 0.03276737482321847 11 0.013910677990988971 12 0.015456308878876635 13 0.013292425635833908 14 0.01530174579008787 15 0.013446988724622673 16 0.017156502855553066 17 0.016692813589186766 18 0.01282873636946761 19 0.022566210963159885 20 0.01576543505645417 21 0.013601551813411439 22 0.015147182701299102 23 0.016383687411609234 24 0.014219804168566505 25 0.013910677990988971 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 646985.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 86.81688961048869 #Duplication Level Percentage of deduplicated Percentage of total 1 94.0861104899446 81.68263468285761 2 4.524185358431416 7.855514016806589 3 0.6848393166267281 1.783668580575155 4 0.23625812759162948 0.82044783130813 5 0.12311863547281496 0.5344388492418687 6 0.07184479305202816 0.37424048804918 7 0.0477316195299153 0.2900737520560952 8 0.03740315861257157 0.259778071388098 9 0.021450789954373863 0.167606177717399 >10 0.14156853761495525 2.3302767091124066 >50 0.012655564621303532 0.7776103027779782 >100 0.011229407481098887 1.9657433357688134 >500 0.0012477119406457053 0.8155134513345004 >1k 3.5648912589877294E-4 0.34245375100630915 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 1191 0.18408463874742073 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1022 0.15796347674211922 No Hit CTGTAGGACGTGGAATATGGCAAGA 975 0.1506990115690472 No Hit GTATCAACGCAGAGTACTTTTTTTT 953 0.14729862361569432 No Hit GTCCTACAGTGTGCATTTCTCATTT 851 0.13153318855924015 No Hit CTTTAGGACGTGAAATATGGCGAGG 794 0.12272309249828049 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 1.5456308878876636E-4 0.0 5 0.0 0.0 0.0 1.5456308878876636E-4 0.0 6 1.5456308878876636E-4 0.0 0.0 1.5456308878876636E-4 0.0 7 1.5456308878876636E-4 0.0 0.0 1.5456308878876636E-4 0.0 8 1.5456308878876636E-4 0.0 0.0 1.5456308878876636E-4 0.0 9 1.5456308878876636E-4 0.0 0.0 1.5456308878876636E-4 0.0 10 1.5456308878876636E-4 0.0 0.0 1.5456308878876636E-4 1.5456308878876636E-4 11 1.5456308878876636E-4 0.0 0.0 1.5456308878876636E-4 1.5456308878876636E-4 12 1.5456308878876636E-4 0.0 0.0 1.5456308878876636E-4 1.5456308878876636E-4 13 1.5456308878876636E-4 0.0 0.0 1.5456308878876636E-4 1.5456308878876636E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATTAGAC 65 5.273278E-5 13.198282 3 TTAGACT 65 5.3196145E-5 13.1859865 4 ATGTCGA 65 5.54066E-5 13.128908 17 TGTCGAG 65 5.54066E-5 13.128908 18 CTCTTAC 60 3.9404692E-4 12.721319 1 TCCAACG 60 4.1522217E-4 12.6426525 18 CCCTTAT 70 1.0460793E-4 12.266986 1 CTTAGAC 55 0.0029886344 12.131754 3 GTCCTAA 310 0.0 12.00318 1 TTAGGAC 360 0.0 11.915115 3 TAGGACC 240 0.0 11.904016 4 ACAATAG 105 2.594079E-7 11.801638 3 ATAAGAC 65 7.7886984E-4 11.731806 3 AGGACGT 560 0.0 11.558517 5 AAATGTC 190 0.0 11.522733 7 CTGTAGG 715 0.0 11.342435 1 GTATGGG 85 5.08346E-5 11.224692 1 TAAGACT 85 5.1810057E-5 11.203779 4 GATATAC 205 0.0 11.16994 1 GACGTGA 300 0.0 11.105242 7 >>END_MODULE