FastQCFastQC Report
Thu 26 May 2016
SRR1926902_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926902_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences646985
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT15520.23988191380016538No Hit
GTCCTACAGTGGACATTTCTAAATT11820.18269357094832184No Hit
CTGTAGGACGTGGAATATGGCAAGA10460.16167299087304962No Hit
GTCCTAAAGTGTGTATTTCTCATTT9920.15332658407845623No Hit
GGTATCAACGCAGAGTACTTTTTTT9490.14668037126053926No Hit
CTTTAGGACGTGAAATATGGCGAGG9470.14637124508296173No Hit
GTCCTACAGTGTGCATTTCTCATTT8770.1355518288677481No Hit
TATCAACGCAGAGTACTTTTTTTTT8230.12720542207315472No Hit
CTGTAGGACCTGGAATATGGCGAGA6840.10572115273151618No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACTCCG456.8081473E-414.7624467
CGAAATC456.812139E-414.76130613
TCCAACG500.001509658213.28620318
TTACACC604.013278E-412.6937554
GCACTGT1155.138645E-912.41684156
GTCCTAT853.813826E-612.3280571
CAATACT550.003016447912.1167674
ACGAAAT550.003090824712.07743212
TAGGACT951.0102267E-612.0256634
TAATTCT802.7984177E-511.9003944
GTCCTAC5250.011.7943741
TCCTAGG657.829064E-411.7245862
TAGGACC3200.011.6028854
GGCGAGG2550.011.53703819
CATAAGC752.0295582E-411.4243793
AAACCAT1001.876575E-611.4234946
CTAAGGT600.005776748511.1070364
ACAATAC600.005776748511.1070363
GTAAGAC600.005776748511.1070363
TGGCGAG6750.011.10698318