FastQCFastQC Report
Thu 26 May 2016
SRR1926901_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926901_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences590787
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT9950.1684194134264972No Hit
GTCCTACAGTGTGCATTTCTCATTT9360.1584327346404034No Hit
GTATCAACGCAGAGTACTTTTTTTT8290.14032130023172482No Hit
CTGTAGGACGTGGAATATGGCAAGA8040.13608965667829523No Hit
GTCCTAAAGTGTGTATTTCTCATTT6820.1154392361375589No Hit
GTACATGGGAAGCAGTGGTATCAAC6710.11357731297404985No Hit
CTGTAGGACCTGGAATATGGCGAGA6180.10460622864077918No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGCAG704.2712054E-714.9744883
TAGGACC3050.013.7365554
GTGTAGG1051.2933015E-913.6189621
TCTACAC1750.013.613173
GGACTGC655.3503543E-513.177496
GGACCTG4100.012.9989336
GATATAC1550.012.9160481
GTCTTAC603.9665552E-412.7110321
TAAGACT905.2373434E-712.6959064
AGGACCT4200.012.6905125
TCCAACG905.496895E-712.64538818
CGTGAAA2250.012.2259459
GACGTGA1950.012.201387
GCATTAG550.002985329812.1332571
TAGTCCT550.00301208212.1188194
CCAACGA550.003103402712.07059919
GAACAGT1750.011.9643246
TGTAGGA6700.011.9470812
GTATTAA802.7587514E-511.9165931
TAATGTG802.8047665E-511.8973565
Produced by FastQC (version 0.11.5)