##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926901_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 590787 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.46567375382329 31.0 28.0 31.0 12.0 34.0 2 27.426693546066517 31.0 28.0 31.0 15.0 34.0 3 27.39851418531552 31.0 28.0 31.0 10.0 34.0 4 30.998676341896488 35.0 32.0 37.0 16.0 37.0 5 30.654288262944174 35.0 31.0 37.0 10.0 37.0 6 30.128098620992 35.0 30.0 37.0 10.0 37.0 7 30.28429704783619 35.0 30.0 37.0 10.0 37.0 8 30.01792693474975 35.0 30.0 37.0 10.0 37.0 9 32.206825810317426 37.0 32.0 39.0 10.0 39.0 10 32.390958162586514 37.0 32.0 39.0 10.0 39.0 11 32.571471613288715 37.0 33.0 39.0 10.0 39.0 12 32.43491647581954 37.0 32.0 39.0 10.0 39.0 13 32.55045557874496 37.0 33.0 39.0 10.0 39.0 14 33.507981726070476 39.0 33.0 40.0 8.0 41.0 15 33.076745087485 38.0 32.0 40.0 2.0 41.0 16 33.1517399671963 38.0 32.0 40.0 2.0 41.0 17 33.36992520146855 39.0 33.0 40.0 2.0 41.0 18 33.40687760563452 39.0 33.0 40.0 2.0 41.0 19 32.792280466564094 39.0 32.0 40.0 2.0 41.0 20 33.07508628321205 39.0 32.0 40.0 2.0 41.0 21 33.05332378674548 39.0 33.0 40.0 2.0 41.0 22 33.074473541225515 39.0 33.0 40.0 2.0 41.0 23 33.05207799088335 39.0 33.0 40.0 2.0 41.0 24 31.773740789827805 38.0 31.0 40.0 2.0 41.0 25 32.32434362976843 38.0 32.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.1870017923584939 1101 2 -0.017426490189567545 1101 3 -1.0071879073974443 1101 4 -0.06893281501863413 1101 5 -0.05543911878908503 1101 6 -2.140052710054917 1101 7 0.026723648082377593 1101 8 0.48981591209239284 1101 9 0.4208118018514142 1101 10 -8.658008077335769 1101 11 -3.5188008005926434 1101 12 -1.1031667769920865 1101 13 -0.2228485533107687 1101 14 0.17093720327955708 1101 15 -0.9974613501992451 1101 16 0.2404543042524807 1101 17 0.4579747248798256 1101 18 0.5916571349803306 1101 19 -0.3625073040907978 1101 20 0.37748840178070964 1101 21 0.40046595857106837 1101 22 0.587602351272821 1101 23 0.29517912111165856 1101 24 0.4973945570872651 1101 25 0.6316335100536321 1102 1 0.004853032925005607 1102 2 -0.48084042936476834 1102 3 -0.4941065317702389 1102 4 -0.26922806523505116 1102 5 -0.3869470387133198 1102 6 -1.192489474134252 1102 7 -0.16723647685622467 1102 8 0.3617619855087497 1102 9 0.16300246076414027 1102 10 0.26636526222097956 1102 11 -0.07935965932030342 1102 12 -0.0972210672111089 1102 13 -0.1818595193597119 1102 14 -0.37104911426096976 1102 15 -0.10637014556208158 1102 16 -0.04441195119866137 1102 17 -0.08709570912171216 1102 18 -0.3265086230501808 1102 19 0.05630024324285898 1102 20 -0.18457791554271097 1102 21 -0.18728821305940357 1102 22 -0.6211365666677011 1102 23 -1.1162010379652507 1102 24 -4.678917040438932 1102 25 -2.5723700068974757 1103 1 -0.030273036807315634 1103 2 -0.43763528151736253 1103 3 0.11459304580860774 1103 4 -0.2535596330084786 1103 5 -0.5617675644416131 1103 6 0.17439861709120663 1103 7 0.32884025933848093 1103 8 0.35241892700451416 1103 9 0.4258501921655764 1103 10 0.3218600202590167 1103 11 0.11554228644344988 1103 12 -0.15553652607429314 1103 13 0.14410789073900077 1103 14 0.10967108097202072 1103 15 0.42257281663889046 1103 16 0.36624214497328467 1103 17 0.06851067315848525 1103 18 -0.21127574423908868 1103 19 -1.9085052898176116 1103 20 -0.5373652385307395 1103 21 -0.04704110762921232 1103 22 0.20820966546694564 1103 23 -0.13605537927278277 1103 24 1.649135116535394 1103 25 0.9398457343093796 1104 1 -0.6302284080138705 1104 2 -0.38004838663129803 1104 3 -1.5392266538658639 1104 4 -0.8271840160810378 1104 5 -0.8753005897487647 1104 6 -2.0722515981770933 1104 7 -0.6183034978808806 1104 8 -0.39549402059603267 1104 9 -0.2651347915580402 1104 10 -0.015586162666529901 1104 11 -0.10402350164854113 1104 12 -0.1753385842492392 1104 13 -0.41836979486427595 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0.15558062911755854 1107 11 -0.20897199839343017 1107 12 0.13927184931340975 1107 13 -0.11468641989350203 1107 14 0.019034533081224936 1107 15 -1.534225916100052 1107 16 -0.4377994248744983 1107 17 -0.4645629979063983 1107 18 -0.48274010636047393 1107 19 0.5477905818594095 1107 20 0.3865223253185377 1107 21 0.15391650361440412 1107 22 0.16763786253730473 1107 23 -0.036093509704450355 1107 24 -4.210709956193924 1107 25 -2.0450185315902445 1108 1 0.7862798028763649 1108 2 0.20766614602705502 1108 3 0.9172247598889207 1108 4 1.0104873013655613 1108 5 1.0316261913353202 1108 6 -2.5204303856817205 1108 7 -0.9540856932136386 1108 8 0.8629329456961017 1108 9 0.8387545774998628 1108 10 1.137901922282225 1108 11 0.9551566440484649 1108 12 1.225081220664947 1108 13 1.1398843105808396 1108 14 0.8596120278755848 1108 15 0.11198690722120119 1108 16 1.1496071444597789 1108 17 0.6673809617483997 1108 18 0.9294272443610936 1108 19 0.9481035219313867 1108 20 -0.4215081983746387 1108 21 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1.6447903970999889 1110 9 1.336777504896446 1110 10 1.4608862813357035 1110 11 1.3235543370681881 1110 12 1.193222582542809 1110 13 1.0503345514846458 1110 14 0.9441490890865154 1110 15 1.493960657013531 1110 16 1.5632733918193296 1110 17 0.7327382826132833 1110 18 1.0817164238641013 1110 19 -0.8383512759704956 1110 20 0.8775234395338884 1110 21 0.6257299305008175 1110 22 1.100389879834843 1110 23 1.124801789536889 1110 24 0.03410160893562875 1110 25 0.6905244650576492 1111 1 0.0878941381738052 1111 2 -0.18788290093857185 1111 3 0.4684522312632957 1111 4 0.16456292144327733 1111 5 0.43362450781422623 1111 6 0.5942704433947625 1111 7 0.752242501152331 1111 8 0.9993442783298896 1111 9 0.4078965582892735 1111 10 0.31665265738133286 1111 11 0.03542024194059934 1111 12 0.2769209863557123 1111 13 0.14331477308790141 1111 14 0.15788699530060768 1111 15 -0.9123814840936717 1111 16 -0.5719719060804493 1111 17 -0.27560186864261027 1111 18 -0.12173680669802422 1111 19 0.4909906453011956 1111 20 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-2.9075521544185925 2216 21 -5.4025270889376245 2216 22 -4.0639492130377235 2216 23 -3.6888591175905425 2216 24 -4.003593855427223 2216 25 -3.4910327703635176 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 43817.0 3 1.0 4 386.0 5 2144.0 6 2529.0 7 1829.0 8 1512.0 9 1394.0 10 1319.0 11 1325.0 12 1214.0 13 1219.0 14 1219.0 15 1313.0 16 1400.0 17 1460.0 18 1734.0 19 2074.0 20 2659.0 21 3467.0 22 4289.0 23 5118.0 24 5887.0 25 6648.0 26 7710.0 27 8079.0 28 9174.0 29 10826.0 30 12919.0 31 15758.0 32 19725.0 33 25595.0 34 34457.0 35 48066.0 36 69127.0 37 108214.0 38 125180.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.834436501932096 18.896243346214 12.918098637182492 25.35122151467141 2 14.160952345442157 19.966002189689476 42.77843121968415 23.094614245184207 3 19.033737496313723 24.45095639853159 29.745807811859137 26.769498293295552 4 11.170301963687118 16.879273810108593 39.97787824225188 31.97254598395241 5 12.941674426479302 38.062981461102176 36.42512486243968 12.570219249978837 6 31.06008275600523 37.67412960592701 18.609265692827588 12.656521945240176 7 27.24051398029108 31.126776824704738 23.24015403109146 18.392555163912718 8 23.941947597334032 38.05542446023624 21.66456725529408 16.338060687135645 9 25.666819598528466 15.807285538458412 20.564877251018746 37.96101761199437 10 14.007436857825972 30.034441185833394 35.31423772207284 20.643884234267794 11 34.05874754738893 23.12725475159007 24.364267974951627 18.44972972606937 12 22.493600682593858 25.24886234357224 32.65361747656969 19.603919497264208 13 28.76371891606385 22.380941901035893 25.720544642872255 23.134794540028 14 19.75933352858422 22.96827624502068 28.297609756675634 28.974780469719462 15 22.887890828016204 31.750073218091725 23.245432122173483 22.116603831718585 16 21.011153398163447 29.099060053633828 28.84917517674784 21.040611371454883 17 21.008285281858722 29.214167933555224 28.509902046095558 21.267644738490503 18 20.781206090380596 28.505574722956755 30.229286509949176 20.483932676713472 19 23.262483873480043 27.43827902519631 27.62282397797651 21.67641312334714 20 22.58300434067162 28.239590175590664 28.841934544568065 20.335470939169653 21 22.317551519296565 27.492420022989634 27.53778984630126 22.652238611412542 22 21.868662073168668 28.869119165721447 28.182803162403292 21.079415598706596 23 21.702187452914995 28.415387506412028 28.087464257720246 21.79496078295273 24 22.63263924157779 27.642288809886573 28.629930590824443 21.09514135771119 25 22.02275951501444 27.991630240833725 28.56027953370419 21.42533071044764 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 174.0 1 174.0 2 212.5 3 251.0 4 251.0 5 251.0 6 436.0 7 621.0 8 621.0 9 621.0 10 948.0 11 1275.0 12 1275.0 13 1275.0 14 2242.0 15 3209.0 16 3209.0 17 3209.0 18 5510.0 19 7811.0 20 7811.0 21 7811.0 22 12082.5 23 16354.0 24 16354.0 25 16354.0 26 23252.5 27 30151.0 28 30151.0 29 30151.0 30 39109.5 31 48068.0 32 48068.0 33 48068.0 34 54567.0 35 61066.0 36 61066.0 37 61066.0 38 65609.0 39 70152.0 40 70152.0 41 70152.0 42 73115.0 43 76078.0 44 76078.0 45 76078.0 46 77461.0 47 78844.0 48 78844.0 49 78844.0 50 74714.0 51 70584.0 52 70584.0 53 70584.0 54 60968.5 55 51353.0 56 51353.0 57 51353.0 58 43326.5 59 35300.0 60 35300.0 61 35300.0 62 28128.0 63 20956.0 64 20956.0 65 20956.0 66 15949.0 67 10942.0 68 10942.0 69 10942.0 70 7899.0 71 4856.0 72 4856.0 73 4856.0 74 3324.0 75 1792.0 76 1792.0 77 1792.0 78 1227.0 79 662.0 80 662.0 81 662.0 82 431.0 83 200.0 84 200.0 85 200.0 86 127.5 87 55.0 88 55.0 89 55.0 90 37.0 91 19.0 92 19.0 93 19.0 94 11.5 95 4.0 96 4.0 97 4.0 98 7.0 99 10.0 100 10.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2154752897406341 2 0.12678004086075015 3 0.12881122976639633 4 0.07075308021334253 5 0.023189406672794087 6 0.02285087518851972 7 0.3072173219789873 8 0.29333753112373834 9 0.018619231635090144 10 0.03639213455949437 11 0.01523391679234648 12 0.01675730847158113 13 0.014895385308072114 14 0.015910979760895214 15 0.014556853823797749 16 0.019465560345776057 17 0.017095839955855494 18 0.014387588081660565 19 0.02454353260989155 20 0.016080245503032393 21 0.014556853823797749 22 0.016418776987306764 23 0.01692657421371831 24 0.01472611956593493 25 0.015064651050209295 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 590787.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 87.81156863218727 #Duplication Level Percentage of deduplicated Percentage of total 1 94.66167200020976 83.1238990768402 2 4.114375441876781 7.225795229858978 3 0.595415446675891 1.5685309308133337 4 0.22302654014825335 0.7833724134811048 5 0.09564942867898976 0.4199563185537306 6 0.057604495253100574 0.3035004653064088 7 0.04372593409336581 0.2687750003852263 8 0.02873109678852703 0.20183381420190055 9 0.02655316311485114 0.20985074147352734 >10 0.12796224719133076 2.1251636643957954 >50 0.011387602526089677 0.6474328287952401 >100 0.012352536393973957 2.088039024785165 >500 0.0015440670490879825 1.033850491109373 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 995 0.1684194134264972 No Hit GTCCTACAGTGTGCATTTCTCATTT 936 0.1584327346404034 No Hit GTATCAACGCAGAGTACTTTTTTTT 829 0.14032130023172482 No Hit CTGTAGGACGTGGAATATGGCAAGA 804 0.13608965667829523 No Hit GTCCTAAAGTGTGTATTTCTCATTT 682 0.1154392361375589 No Hit GTACATGGGAAGCAGTGGTATCAAC 671 0.11357731297404985 No Hit CTGTAGGACCTGGAATATGGCGAGA 618 0.10460622864077918 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTAGCAG 70 4.2712054E-7 14.974488 3 TAGGACC 305 0.0 13.736555 4 GTGTAGG 105 1.2933015E-9 13.618962 1 TCTACAC 175 0.0 13.61317 3 GGACTGC 65 5.3503543E-5 13.17749 6 GGACCTG 410 0.0 12.998933 6 GATATAC 155 0.0 12.916048 1 GTCTTAC 60 3.9665552E-4 12.711032 1 TAAGACT 90 5.2373434E-7 12.695906 4 AGGACCT 420 0.0 12.690512 5 TCCAACG 90 5.496895E-7 12.645388 18 CGTGAAA 225 0.0 12.225945 9 GACGTGA 195 0.0 12.20138 7 GCATTAG 55 0.0029853298 12.133257 1 TAGTCCT 55 0.003012082 12.118819 4 CCAACGA 55 0.0031034027 12.070599 19 GAACAGT 175 0.0 11.964324 6 TGTAGGA 670 0.0 11.947081 2 GTATTAA 80 2.7587514E-5 11.916593 1 TAATGTG 80 2.8047665E-5 11.897356 5 >>END_MODULE