FastQCFastQC Report
Thu 26 May 2016
SRR1926901_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926901_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences590787
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT12740.21564455548277128No Hit
GTCCTACAGTGGACATTTCTAAATT10490.17755976350190508No Hit
GTCCTACAGTGTGCATTTCTCATTT9990.1690964763950459No Hit
CTGTAGGACGTGGAATATGGCAAGA9820.16621895877871382No Hit
GGTATCAACGCAGAGTACTTTTTTT8880.1503079790178186No Hit
CTGTAGGACCTGGAATATGGCGAGA7810.13219654460914002No Hit
GTCCTAAAGTGTGTATTTCTCATTT7490.12678004086075015No Hit
GTACATGGGAAGCAGTGGTATCAAC7180.12153280285449747No Hit
CTTTAGGACGTGAAATATGGCGAGG7140.12085573988594873No Hit
TATCAACGCAGAGTACTTTTTTTTT7000.11848601949602819No Hit
CCCATGTACTCTGCGTTGATACCAC6380.10799154348352283No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACTTC350.002132250516.3263536
CTAGGAC1050.016.3263533
TAGGACC2950.014.8505254
CTCGTCT400.00532050514.23114613
GTCCTAA2400.013.90173151
TAGGTCT500.001469752213.3331894
CTACACC500.001469752213.3331894
TGTAGGA7300.012.7950342
CTGTAGG7250.012.75398351
GTCTAAG603.9688725E-412.7101551
AGGACTT905.22552E-712.6982765
TAGGACA1800.012.6982764
CTAGTAC604.0003183E-412.6982753
GGCGAGG2250.012.65097819
GGCGAGA4150.012.57476719
GTAGGAC6900.012.4222263
TTAGGAC3100.012.2886533
TAAAGTG2950.012.2678245
CTAAAGT2650.012.2190954
TCCTAAA2900.012.1602342
Produced by FastQC (version 0.11.5)