FastQCFastQC Report
Thu 26 May 2016
SRR1926900_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926900_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences613505
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT9390.15305498732691666No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGACT759.4184725E-713.9627414
CTCCTAG1009.327778E-913.3685521
TAGACTG1051.9423169E-812.6861335
CCTAGAC550.002972650812.1402753
GTATAAA959.739197E-712.06185151
CTAGGAC802.742876E-511.9234823
GTATTAT907.02577E-611.67095851
TAGGACG2400.011.5033954
GTATTAG751.9733448E-411.4587591
TAGGACC1501.6916601E-1011.4240614
TTAGGAC1950.011.2508773
GTCTAGG600.00564834511.140461
GTATAAT600.00564834511.140461
TTTACCC600.00569339111.1285843
TTATATC600.00569339111.1285843
ACTGATC600.005841030711.0903888
GCGTCAG600.005952093311.06236411
TAGGGCC700.001463194710.8800584
CACTGTT1752.0008883E-1110.8738297
GGGACTC700.00147020510.8738295
Produced by FastQC (version 0.11.5)