##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926900_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 613505 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.83377641584013 31.0 28.0 31.0 16.0 34.0 2 27.811748885502155 31.0 28.0 31.0 16.0 34.0 3 27.757066364577305 31.0 28.0 31.0 15.0 34.0 4 31.377983879511984 35.0 32.0 37.0 17.0 37.0 5 31.09107994229876 35.0 32.0 37.0 16.0 37.0 6 30.54075353909096 35.0 30.0 37.0 11.0 37.0 7 30.673231677003447 35.0 31.0 37.0 11.0 37.0 8 30.401106755446165 35.0 30.0 37.0 10.0 37.0 9 32.654925387731154 37.0 32.0 39.0 17.0 39.0 10 32.84476410135207 37.0 33.0 39.0 15.0 39.0 11 33.01666001092086 37.0 33.0 39.0 17.0 39.0 12 32.90185573059714 37.0 33.0 39.0 11.0 39.0 13 33.01411235442254 38.0 33.0 39.0 13.0 39.0 14 34.00906268082575 39.0 34.0 41.0 11.0 41.0 15 33.571043430778886 39.0 33.0 40.0 10.0 41.0 16 33.64548944181384 39.0 33.0 40.0 10.0 41.0 17 33.8621396728633 39.0 33.0 41.0 10.0 41.0 18 33.91422074799716 39.0 34.0 41.0 10.0 41.0 19 33.29149395685447 39.0 33.0 40.0 8.0 41.0 20 33.556843057513795 39.0 33.0 41.0 2.0 41.0 21 33.54875836382752 39.0 33.0 41.0 2.0 41.0 22 33.56263111140088 39.0 34.0 41.0 2.0 41.0 23 33.55667353974295 39.0 34.0 41.0 2.0 41.0 24 32.260121759398864 38.0 32.0 40.0 2.0 41.0 25 32.832314325066626 39.0 33.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.03726090209845623 1101 2 -0.28045398824341916 1101 3 -0.9516495613231335 1101 4 -0.4123116863645002 1101 5 -0.457018913123143 1101 6 -2.504000209641749 1101 7 -0.8178355522080594 1101 8 -0.453422498546761 1101 9 -0.36096982950319045 1101 10 -8.560532728992968 1101 11 -3.9224869634797876 1101 12 -1.5864459120753764 1101 13 -0.9770947483493302 1101 14 -0.7999151460709513 1101 15 -1.9948705103704931 1101 16 -0.4443073598279881 1101 17 -0.3391228466593219 1101 18 -0.1606608489809318 1101 19 -1.1368412062358857 1101 20 -0.5276559962991172 1101 21 -0.5689003328277664 1101 22 -0.4480206585293658 1101 23 -0.3532698138438022 1101 24 -0.716589050036486 1101 25 -0.5726125414491676 1102 1 0.058516743679188465 1102 2 -0.03283494062437242 1102 3 -0.24551155518512857 1102 4 -0.09084846490128129 1102 5 -0.06453054563477423 1102 6 -0.9201050507465887 1102 7 -0.05370328807579483 1102 8 0.6184763233520592 1102 9 0.5939603754270095 1102 10 0.47775803429779273 1102 11 0.46129187029904273 1102 12 0.5491272984978366 1102 13 0.3510782048236223 1102 14 -0.09725451841032395 1102 15 0.4803695648695836 1102 16 0.2758812353606075 1102 17 0.3914038338673578 1102 18 0.3309414676213862 1102 19 0.5774271830324977 1102 20 0.30993434129121766 1102 21 0.28749012356269077 1102 22 0.2325827470740407 1102 23 -0.30815794373192773 1102 24 -3.8051372135846506 1102 25 -1.7780278468644752 1103 1 0.19089693568739108 1103 2 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-0.020875011574720048 2215 4 0.1979805383517288 2215 5 0.5253295225650803 2215 6 1.189726586311572 2215 7 0.3826580490599838 2215 8 -0.7067920718582528 2215 9 -0.4264145493525575 2215 10 -0.33666008663048785 2215 11 -0.4483542230353379 2215 12 -0.7238149322130312 2215 13 -0.348359495219114 2215 14 -0.2809678784305589 2215 15 -0.3791273957890411 2215 16 -0.035299136686177235 2215 17 -0.68340015695793 2215 18 -1.258116249340496 2215 19 -0.01822158771307869 2215 20 -3.8166627301267617 2215 21 -2.5371917703350775 2215 22 -1.4504067525901903 2215 23 -1.2995119550520613 2215 24 -1.538221338367709 2215 25 -1.305857225129479 2216 1 -0.8126289302966718 2216 2 -1.3501305381086475 2216 3 -0.6024892876345582 2216 4 -0.7331908072436235 2216 5 -0.584439605638174 2216 6 -1.5721320777736167 2216 7 -8.825486391934373 2216 8 -9.351317999272453 2216 9 -5.033984550631121 2216 10 -3.2431369172575337 2216 11 -2.428033303931791 2216 12 -2.2941796877336813 2216 13 -1.9483056129564211 2216 14 -1.765455266328054 2216 15 -3.0386088475050563 2216 16 -2.2542182037011003 2216 17 -2.210312977283415 2216 18 -2.0469142182531677 2216 19 -1.4452695703246299 2216 20 -2.3040296226727435 2216 21 -5.3057446206154495 2216 22 -3.7006953423361217 2216 23 -3.291130066798395 2216 24 -3.7167341879174742 2216 25 -3.1167240902776996 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 40395.0 3 3.0 4 342.0 5 1997.0 6 2394.0 7 1781.0 8 1546.0 9 1361.0 10 1296.0 11 1306.0 12 1213.0 13 1181.0 14 1167.0 15 1247.0 16 1366.0 17 1501.0 18 1628.0 19 2071.0 20 2541.0 21 3465.0 22 4205.0 23 5057.0 24 5706.0 25 6647.0 26 7512.0 27 8284.0 28 9200.0 29 10771.0 30 12805.0 31 15867.0 32 20225.0 33 26525.0 34 35591.0 35 49812.0 36 71624.0 37 113928.0 38 139945.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.50692521680609 18.402177126774184 12.555069317508 25.53582833891173 2 14.933503932317462 20.004308494752284 40.97998671547452 24.082200857455735 3 18.871730594099155 24.318210749992655 29.68733169475426 27.122726961153926 4 11.909103958554903 16.495174960688487 38.934380770309865 32.66134031044675 5 13.611241538521735 37.59570111254582 35.614345710894604 13.178711638037848 6 31.76362409148268 36.796377995376304 18.191051939963675 13.248945973177339 7 27.677242985000444 31.256601342668134 22.995562589721036 18.070593082610387 8 24.9394261424017 35.99149840595112 21.563802828414943 17.50527262323224 9 26.157583266763396 15.818647190205255 20.050538808914396 37.97323073411696 10 14.650797998969484 29.138213291069064 34.58707548215835 21.623913227803108 11 34.383100263121 22.953802212563946 23.82369914868782 18.839398375627237 12 22.788192389643967 24.951866723399537 31.473049440741047 20.786891446215446 13 28.49643794525684 22.008281573498962 25.558924699629937 23.936355781614253 14 20.87763572753742 21.731241379984937 27.78424448726602 29.606878405211624 15 23.597190795093674 29.995663599668738 23.2788077181405 23.12833788709709 16 22.398131030052852 28.385714704932948 27.009908931733033 22.206245333281164 17 22.102469379337553 28.361425709129385 27.18534446920653 22.350760442326532 18 21.82777941706295 27.999171844000188 28.437377223427685 21.73567151550918 19 23.72115923882563 27.07004604087595 27.234058509736407 21.974736210562014 20 23.320834691881316 27.218617541571565 27.6957939354418 21.764753831105317 21 23.32619834400865 26.72507250464209 27.091549031722717 22.85718011962655 22 22.954892623609602 27.355425389181338 27.359501013200134 22.330180974008933 23 22.725612817820785 27.327533925881486 27.176571089475644 22.770282166822085 24 23.2461424344438 27.067272030713802 27.368052102607578 22.318533432234823 25 22.827873128488708 27.20081903072669 27.444702384057596 22.526605456727005 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 149.0 1 149.0 2 226.5 3 304.0 4 304.0 5 304.0 6 480.5 7 657.0 8 657.0 9 657.0 10 972.5 11 1288.0 12 1288.0 13 1288.0 14 2080.5 15 2873.0 16 2873.0 17 2873.0 18 5001.5 19 7130.0 20 7130.0 21 7130.0 22 10947.0 23 14764.0 24 14764.0 25 14764.0 26 20608.5 27 26453.0 28 26453.0 29 26453.0 30 34401.0 31 42349.0 32 42349.0 33 42349.0 34 49061.5 35 55774.0 36 55774.0 37 55774.0 38 61469.0 39 67164.0 40 67164.0 41 67164.0 42 71512.5 43 75861.0 44 75861.0 45 75861.0 46 78891.5 47 81922.0 48 81922.0 49 81922.0 50 79478.5 51 77035.0 52 77035.0 53 77035.0 54 69183.5 55 61332.0 56 61332.0 57 61332.0 58 52559.0 59 43786.0 60 43786.0 61 43786.0 62 35873.5 63 27961.0 64 27961.0 65 27961.0 66 21705.0 67 15449.0 68 15449.0 69 15449.0 70 11197.5 71 6946.0 72 6946.0 73 6946.0 74 4834.5 75 2723.0 76 2723.0 77 2723.0 78 1924.5 79 1126.0 80 1126.0 81 1126.0 82 725.5 83 325.0 84 325.0 85 325.0 86 212.0 87 99.0 88 99.0 89 99.0 90 63.5 91 28.0 92 28.0 93 28.0 94 16.0 95 4.0 96 4.0 97 4.0 98 3.5 99 3.0 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.21548316639636186 2 0.12420436671257773 3 0.12697533027440688 4 0.07383802902991826 5 0.022330706351211482 6 0.023308693490680595 7 0.307576955363037 8 0.30236102395253506 9 0.01874475350649139 10 0.036022526303779105 11 0.016136787801240414 12 0.016951777084131345 13 0.01548479637492767 14 0.017440770653865904 15 0.015158800661771298 16 0.019396744932804137 17 0.01825575993675683 18 0.015321798518349484 19 0.022330706351211482 20 0.01711477494070953 21 0.015647794231505856 22 0.016625781370974972 23 0.017766766367022273 24 0.0162997856578186 25 0.01581079208808404 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 613505.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 89.14350022404393 #Duplication Level Percentage of deduplicated Percentage of total 1 94.10157678484515 83.88543931202732 2 4.533750457662694 8.083087698768274 3 0.7125747704037948 1.905642276154162 4 0.2481796300393347 0.8849440362405829 5 0.11980374784710508 0.5339862711524858 6 0.06249749538179307 0.3342747296141433 7 0.04576000076204014 0.28554446467282235 8 0.03482240003540679 0.24833525002864262 9 0.020860918414078188 0.1673653756787227 >10 0.10443025012205732 1.6506075678421248 >50 0.00750570677106572 0.4567713713381196 >100 0.00787171159508224 1.3211766830993752 >500 3.661261203964635E-4 0.24282496338319318 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 939 0.15305498732691666 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 1.62997856578186E-4 0.0 0.0 0.0 6 0.0 1.62997856578186E-4 0.0 0.0 0.0 7 0.0 1.62997856578186E-4 0.0 0.0 0.0 8 0.0 1.62997856578186E-4 0.0 0.0 0.0 9 0.0 1.62997856578186E-4 0.0 0.0 0.0 10 0.0 1.62997856578186E-4 0.0 0.0 0.0 11 0.0 1.62997856578186E-4 0.0 0.0 0.0 12 0.0 1.62997856578186E-4 0.0 0.0 0.0 13 0.0 1.62997856578186E-4 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTAGACT 75 9.4184725E-7 13.962741 4 CTCCTAG 100 9.327778E-9 13.368552 1 TAGACTG 105 1.9423169E-8 12.686133 5 CCTAGAC 55 0.0029726508 12.140275 3 GTATAAA 95 9.739197E-7 12.0618515 1 CTAGGAC 80 2.742876E-5 11.923482 3 GTATTAT 90 7.02577E-6 11.6709585 1 TAGGACG 240 0.0 11.503395 4 GTATTAG 75 1.9733448E-4 11.458759 1 TAGGACC 150 1.6916601E-10 11.424061 4 TTAGGAC 195 0.0 11.250877 3 GTCTAGG 60 0.005648345 11.14046 1 GTATAAT 60 0.005648345 11.14046 1 TTTACCC 60 0.005693391 11.128584 3 TTATATC 60 0.005693391 11.128584 3 ACTGATC 60 0.0058410307 11.090388 8 GCGTCAG 60 0.0059520933 11.062364 11 TAGGGCC 70 0.0014631947 10.880058 4 CACTGTT 175 2.0008883E-11 10.873829 7 GGGACTC 70 0.001470205 10.873829 5 >>END_MODULE