FastQCFastQC Report
Thu 26 May 2016
SRR1926900_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926900_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences613505
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT14620.2383028663173079No Hit
GGTATCAACGCAGAGTACTTTTTTT9690.15794492302426225No Hit
TATCAACGCAGAGTACTTTTTTTTT8130.13251725739806522No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCCAC402.7855605E-416.60310613
AAGGCGT400.005203719314.278956
GTTTAGA759.3058406E-713.9776431
TTTAGAA1101.7644197E-1013.8621252
TATACTG707.095019E-613.5995
TCTAGCC500.001473183913.3292013
CATGCGC655.497072E-513.13973510
GTCCTAA1404.9112714E-1112.2531291
GATATAC1052.5998997E-711.7993091
TTCTAGC752.0097356E-411.4362542
GTCCTAC2800.011.2320351
GTCCCAC600.005731658511.118581
TTAATAC600.005773724511.1076663
AAAGGCG600.00578076111.105855
CCCTATA700.001450228210.891672
TAGGACT700.001464160410.87924
TAGACTG1053.3714168E-610.8791995
CTTACAC803.68812E-410.7109643
TAATACT803.6937013E-410.7092124
CTTAGAC909.2837734E-510.578733
Produced by FastQC (version 0.11.5)