##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926900_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 613505 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.740766578919487 31.0 30.0 33.0 25.0 34.0 2 30.056618935461 31.0 30.0 34.0 26.0 34.0 3 30.118119656726513 31.0 30.0 34.0 26.0 34.0 4 33.72753115296534 35.0 35.0 37.0 28.0 37.0 5 33.67402873652212 37.0 35.0 37.0 28.0 37.0 6 33.57851362254586 37.0 35.0 37.0 28.0 37.0 7 33.565032069828284 37.0 35.0 37.0 28.0 37.0 8 33.58893407551691 37.0 35.0 37.0 28.0 37.0 9 35.11288579555179 39.0 35.0 39.0 29.0 39.0 10 35.0250038711991 39.0 35.0 39.0 28.0 39.0 11 35.03141294691975 39.0 35.0 39.0 28.0 39.0 12 34.9337446312581 39.0 35.0 39.0 27.0 39.0 13 34.94180813522302 39.0 35.0 39.0 27.0 39.0 14 36.03793612114001 40.0 36.0 41.0 27.0 41.0 15 35.992687916153905 40.0 36.0 41.0 27.0 41.0 16 35.97912323452947 40.0 36.0 41.0 27.0 41.0 17 35.95555374446826 40.0 36.0 41.0 27.0 41.0 18 35.93052379361212 40.0 36.0 41.0 27.0 41.0 19 35.994472742683435 40.0 36.0 41.0 27.0 41.0 20 35.949465774525066 40.0 36.0 41.0 27.0 41.0 21 35.906721216616 40.0 36.0 41.0 27.0 41.0 22 35.81418407347943 40.0 36.0 41.0 27.0 41.0 23 35.73906162133968 40.0 36.0 41.0 27.0 41.0 24 35.6468015745593 40.0 36.0 41.0 27.0 41.0 25 35.53167618845812 40.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.1446119884596655 1101 2 0.06822269206395859 1101 3 0.32137175234193194 1101 4 0.09818191105688356 1101 5 0.03052256267140052 1101 6 0.11391876869928552 1101 7 0.06196688067728928 1101 8 0.16079270747903252 1101 9 0.040663199104159276 1101 10 0.0575271226061318 1101 11 0.2743036982204359 1101 12 0.056143278777312844 1101 13 0.11766970518943509 1101 14 0.06346137388611339 1101 15 0.015949327243305333 1101 16 0.18256515631645698 1101 17 0.057236111200538176 1101 18 -0.02726899764970625 1101 19 -0.029898761617943137 1101 20 0.11746784965936286 1101 21 0.12835042255164097 1101 22 -0.1165597220669099 1101 23 -0.023962272198943424 1101 24 0.04098452643996353 1101 25 -0.00795014687237483 1102 1 -0.7686417874894644 1102 2 -0.6534261545848885 1102 3 -0.5151023591321788 1102 4 -0.6240609361859626 1102 5 -0.804993722844884 1102 6 -0.7159124361319229 1102 7 -0.7482647795543684 1102 8 -0.5397072430209136 1102 9 -0.5740952406542803 1102 10 -0.3461631060840986 1102 11 -0.4707883718716346 1102 12 -0.24205244441841245 1102 13 -0.24588835836863154 1102 14 -0.4145259541012152 1102 15 -0.529395193101216 1102 16 -0.39514002138871973 1102 17 -0.38219810323367653 1102 18 -0.4481654435461522 1102 19 -0.7547230364422148 1102 20 -0.7366084794169652 1102 21 -0.6506194814182606 1102 22 -0.3375378430450269 1102 23 -0.3820283052649742 1102 24 -0.39452928407384746 1102 25 -0.6005647394869627 1103 1 -0.8692711367512018 1103 2 -0.7155799385658064 1103 3 -0.6279441396435459 1103 4 -0.5705366788870876 1103 5 -0.7711606556420136 1103 6 -0.7971832583764851 1103 7 -0.6934566891882952 1103 8 -0.5819874882552085 1103 9 -0.6010902334588977 1103 10 -0.4548108676312026 1103 11 -0.49610772842731876 1103 12 -0.38024068736595495 1103 13 -0.45586529372849327 1103 14 -0.6633234386755049 1103 15 -0.49275050683945665 1103 16 -0.5970246193345901 1103 17 -0.41208603273867794 1103 18 -0.46982797040561053 1103 19 -0.4650203140261482 1103 20 -0.622253697939648 1103 21 -0.5115490652690653 1103 22 -0.7245309630797223 1103 23 -0.5269535916016395 1103 24 -0.7638222641436343 1103 25 -0.499149319690801 1104 1 -0.27988816602659483 1104 2 -0.2145991116829542 1104 3 -0.07087784827771415 1104 4 -0.05423615545809923 1104 5 -0.11334392326556753 1104 6 -0.06518516701257937 1104 7 -0.09306773228684051 1104 8 -0.23188289885295887 1104 9 -0.34448953362566215 1104 10 -0.1581794175497464 1104 11 0.019433678372443808 1104 12 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-0.07054604154373578 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 16049.0 3 14.0 4 243.0 5 1901.0 6 2453.0 7 1771.0 8 1472.0 9 1197.0 10 1023.0 11 923.0 12 862.0 13 876.0 14 799.0 15 864.0 16 864.0 17 915.0 18 1007.0 19 1230.0 20 1530.0 21 2011.0 22 2497.0 23 2973.0 24 3652.0 25 4268.0 26 5020.0 27 5444.0 28 6371.0 29 8024.0 30 10009.0 31 12865.0 32 16751.0 33 21746.0 34 29156.0 35 41027.0 36 59983.0 37 103149.0 38 242566.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.89926174452836 19.167716499454958 10.593084811258558 28.339936944758126 2 14.463477435082417 20.85602769990895 39.04665711572414 25.633837749284496 3 18.40698529351829 24.512107048710227 29.37090297895511 27.710004678816375 4 12.160455090015567 17.39496825616743 37.473696220894695 32.97088043292231 5 13.20489602707192 38.44626611918621 34.869440908135104 13.479396945606762 6 31.953601494277876 37.6183147175576 17.495612521446716 12.932471266717807 7 28.21643600223681 31.977931862015446 21.3028613910296 18.50277074471814 8 25.662079465837255 37.024774796803634 19.595530820968758 17.71761491639036 9 26.189137157286098 15.87548435159239 19.57831865403644 38.357059837085075 10 14.763571280703786 29.72747411093706 33.65129922100531 21.857655387353848 11 34.444486101353434 23.126050365376372 23.50291860982563 18.926544923444563 12 22.84169055959159 25.36556582629857 31.015434367670142 20.777309246439696 13 28.776365118174407 21.861450692746537 25.496495517522412 23.865688671556644 14 21.08902305445441 21.915586532443147 27.364939755894014 29.630450657208428 15 23.257493267958086 30.00404248521559 23.307861329716832 23.430602917109493 16 22.523716381418094 28.605541972290137 26.521597392013042 22.34914425427873 17 21.97886562901893 28.43278679973855 27.051212463508733 22.537135107733786 18 21.677622946410672 28.227429873561327 28.25073879501026 21.844208385017744 19 23.17103583845556 27.366940340960028 27.300925993841922 22.16109782674249 20 23.174572127139363 27.412713936430315 27.67807660961695 21.734637326813367 21 23.262926066664384 26.69591200966368 27.14747053865879 22.89369138501315 22 22.942274309092838 27.49936838330576 27.04704933130129 22.511307976300113 23 22.58306384310453 27.309194398080532 27.247418109743375 22.860323649071564 24 22.839459889422074 26.91685791779679 27.675777175046946 22.567905017734198 25 22.818733964275584 27.065205697388965 27.351607403084728 22.76445293525072 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 227.0 1 227.0 2 218.0 3 209.0 4 209.0 5 209.0 6 388.0 7 567.0 8 567.0 9 567.0 10 914.0 11 1261.0 12 1261.0 13 1261.0 14 2004.0 15 2747.0 16 2747.0 17 2747.0 18 4687.5 19 6628.0 20 6628.0 21 6628.0 22 10444.5 23 14261.0 24 14261.0 25 14261.0 26 20035.0 27 25809.0 28 25809.0 29 25809.0 30 33490.5 31 41172.0 32 41172.0 33 41172.0 34 47900.5 35 54629.0 36 54629.0 37 54629.0 38 60127.5 39 65626.0 40 65626.0 41 65626.0 42 70484.0 43 75342.0 44 75342.0 45 75342.0 46 78889.5 47 82437.0 48 82437.0 49 82437.0 50 80449.5 51 78462.0 52 78462.0 53 78462.0 54 70707.0 55 62952.0 56 62952.0 57 62952.0 58 53999.5 59 45047.0 60 45047.0 61 45047.0 62 36994.5 63 28942.0 64 28942.0 65 28942.0 66 22375.5 67 15809.0 68 15809.0 69 15809.0 70 11405.0 71 7001.0 72 7001.0 73 7001.0 74 4922.5 75 2844.0 76 2844.0 77 2844.0 78 1955.0 79 1066.0 80 1066.0 81 1066.0 82 704.0 83 342.0 84 342.0 85 342.0 86 221.0 87 100.0 88 100.0 89 100.0 90 61.5 91 23.0 92 23.0 93 23.0 94 12.5 95 2.0 96 2.0 97 2.0 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.11556548031393388 2 0.10611160463239909 3 0.016625781370974972 4 0.0 5 0.004563939984189208 6 0.3432734859536597 7 0.021515717068320554 8 0.009616873538112974 9 0.009127879968378416 10 0.003585952844720092 11 0.004563939984189208 12 6.51991426312744E-4 13 8.1498928289093E-4 14 1.62997856578186E-4 15 0.0034229549881419057 16 8.1498928289093E-4 17 3.25995713156372E-4 18 9.77987139469116E-4 19 3.25995713156372E-4 20 8.1498928289093E-4 21 0.012061841386785763 22 0.0 23 1.62997856578186E-4 24 1.62997856578186E-4 25 0.005052933553923766 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 613505.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 86.41941926632468 #Duplication Level Percentage of deduplicated Percentage of total 1 92.43931519308684 79.88551936363308 2 5.796244955206014 10.018162459085357 3 0.9549927137291451 2.475897471721325 4 0.3094670636909257 1.0697585570489805 5 0.13895381915039653 0.6004154177907587 6 0.08769265450748095 0.4547008965875372 7 0.05219937819164436 0.31577279645696116 8 0.03975555590337112 0.27485216430233905 9 0.025743842133870244 0.20022910982037068 >10 0.13847452570880442 2.0975815020550503 >50 0.006977301802541087 0.4323480764745571 >100 0.00942870082338709 1.5623413315203454 >500 5.657220491715088E-4 0.3741666523798627 >1k 1.8857401639050297E-4 0.2382542011234143 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 1462 0.2383028663173079 No Hit GGTATCAACGCAGAGTACTTTTTTT 969 0.15794492302426225 No Hit TATCAACGCAGAGTACTTTTTTTTT 813 0.13251725739806522 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 1.62997856578186E-4 0.0 10 0.0 0.0 0.0 1.62997856578186E-4 0.0 11 0.0 0.0 0.0 1.62997856578186E-4 0.0 12 0.0 0.0 0.0 1.62997856578186E-4 0.0 13 0.0 0.0 0.0 1.62997856578186E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCGCCAC 40 2.7855605E-4 16.603106 13 AAGGCGT 40 0.0052037193 14.27895 6 GTTTAGA 75 9.3058406E-7 13.977643 1 TTTAGAA 110 1.7644197E-10 13.862125 2 TATACTG 70 7.095019E-6 13.599 5 TCTAGCC 50 0.0014731839 13.329201 3 CATGCGC 65 5.497072E-5 13.139735 10 GTCCTAA 140 4.9112714E-11 12.253129 1 GATATAC 105 2.5998997E-7 11.799309 1 TTCTAGC 75 2.0097356E-4 11.436254 2 GTCCTAC 280 0.0 11.232035 1 GTCCCAC 60 0.0057316585 11.11858 1 TTAATAC 60 0.0057737245 11.107666 3 AAAGGCG 60 0.005780761 11.10585 5 CCCTATA 70 0.0014502282 10.89167 2 TAGGACT 70 0.0014641604 10.8792 4 TAGACTG 105 3.3714168E-6 10.879199 5 CTTACAC 80 3.68812E-4 10.710964 3 TAATACT 80 3.6937013E-4 10.709212 4 CTTAGAC 90 9.2837734E-5 10.57873 3 >>END_MODULE