FastQCFastQC Report
Thu 26 May 2016
SRR1926899_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926899_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences607713
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT7210.11864152980107386No Hit
GTATCAACGCAGAGTACTTTTTTTT6730.11074306457159876No Hit
GTCCTACAGTGTGCATTTCTCATTT6140.10103453439370229No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC1900.014.0338034
GCTCTAA551.8906764E-413.8744821
GTCTTAC551.8906764E-413.8744821
GTATTAT852.5598638E-713.466411
TATAGGA801.918881E-613.1070392
AGGACGT2850.013.0238525
CTTTAGG2250.012.29433251
GGACGTG2950.012.2597416
AGGACCT2800.012.2366975
TGATACT550.00300986412.1201034
TTAGGAC2050.012.0898643
TCCTACA5900.011.9558822
GTCCTAG802.7430087E-511.9233831
TAGGACG3200.011.606084
TGTAGGA5050.011.5143762
TCTATAC752.0053987E-411.4388723
GCATTTC3650.011.43057413
GACGTGA1501.6916601E-1011.42091757
GTCCTAC5450.011.3764391
GTAGGAC4800.011.3197173
Produced by FastQC (version 0.11.5)