##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926899_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 607713 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.687345835945585 31.0 28.0 31.0 16.0 34.0 2 27.652400063845928 31.0 28.0 31.0 16.0 34.0 3 27.61156828963672 31.0 28.0 31.0 12.0 34.0 4 31.191552591437077 35.0 32.0 37.0 17.0 37.0 5 30.914420129238636 35.0 32.0 37.0 16.0 37.0 6 30.379412650379372 35.0 30.0 37.0 10.0 37.0 7 30.515259670271988 35.0 31.0 37.0 10.0 37.0 8 30.252504060304783 35.0 30.0 37.0 10.0 37.0 9 32.49611576517205 37.0 32.0 39.0 13.0 39.0 10 32.69725182775422 37.0 33.0 39.0 11.0 39.0 11 32.87471224081104 37.0 33.0 39.0 13.0 39.0 12 32.742656484228576 37.0 33.0 39.0 10.0 39.0 13 32.85456621793511 38.0 33.0 39.0 10.0 39.0 14 33.85205351868398 39.0 33.0 40.0 10.0 41.0 15 33.39755443770333 39.0 33.0 40.0 10.0 41.0 16 33.48031060714515 39.0 33.0 40.0 10.0 41.0 17 33.708538405464424 39.0 33.0 40.0 10.0 41.0 18 33.74633091607387 39.0 33.0 41.0 8.0 41.0 19 33.117422204231275 39.0 32.0 40.0 2.0 41.0 20 33.40122722403503 39.0 33.0 40.0 2.0 41.0 21 33.38976951291152 39.0 33.0 40.0 2.0 41.0 22 33.40858266977998 39.0 33.0 40.0 2.0 41.0 23 33.38434260909344 39.0 33.0 40.0 2.0 41.0 24 32.08255541678391 38.0 31.0 40.0 2.0 41.0 25 32.6521548823211 39.0 32.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.48993243719036883 1101 2 -0.5965340119432803 1101 3 -1.5500135266663904 1101 4 -0.7890259412267646 1101 5 -0.8539732649789329 1101 6 -2.892297798679639 1101 7 -1.3642561966779212 1101 8 -0.8026127022537004 1101 9 -0.7169209548947251 1101 10 -9.432903365324375 1101 11 -4.535403169082802 1101 12 -2.0229742926810843 1101 13 -1.4305824298446588 1101 14 -1.1635181914925568 1101 15 -2.7127500066786467 1101 16 -1.0430323583273733 1101 17 -0.7805850756362744 1101 18 -0.6294681717378694 1101 19 -1.0889380309241332 1101 20 -0.6985823996793101 1101 21 -0.775519933618483 1101 22 -0.5072542459708558 1101 23 -0.8006755559047605 1101 24 -1.0181491606557174 1101 25 -1.0769011349580389 1102 1 7.470325083502871E-5 1102 2 -0.22872863281090616 1102 3 -0.14157277353774944 1102 4 -0.11648406942899925 1102 5 0.16900768131743504 1102 6 -1.201337346702239 1102 7 -0.01877409162574395 1102 8 0.5160213438710457 1102 9 0.4594178778310436 1102 10 0.65535374066733 1102 11 0.4909400481244077 1102 12 0.3644735633074916 1102 13 0.2666465778845577 1102 14 -0.11445584416646426 1102 15 0.49697727857571294 1102 16 0.6166091163616443 1102 17 0.4631632801831813 1102 18 0.345413760440465 1102 19 0.5576226565625575 1102 20 0.36804668571785726 1102 21 0.5237735385150017 1102 22 0.31163660549544403 1102 23 -0.19456985232076107 1102 24 -4.001628434585591 1102 25 -2.139572912677359 1103 1 -0.10611501488756758 1103 2 -0.31506006209579596 1103 3 0.14030755233546088 1103 4 0.012885097471770024 1103 5 -0.692908228411941 1103 6 0.16350330629273557 1103 7 -0.13385353102394149 1103 8 -0.10914330949849571 1103 9 -0.18596090295367418 1103 10 -0.027269476238117818 1103 11 -0.21619233006330774 1103 12 -0.3978637341922777 1103 13 -0.4277317971193426 1103 14 -0.5040616124968338 1103 15 0.011863191738619605 1103 16 0.05249262605356364 1103 17 -0.3418559163707968 1103 18 -0.28746697121356135 1103 19 -1.7499467163500135 1103 20 -0.5022158232561367 1103 21 -0.6129995675810704 1103 22 -0.45728058400419513 1103 23 -0.2303285063770062 1103 24 1.1627502525574016 1103 25 0.4260168741316406 1104 1 -0.23539678284336674 1104 2 -0.39572304552121196 1104 3 -1.6055488012557007 1104 4 -0.6887723610917647 1104 5 -0.4177701068649817 1104 6 -2.1480989036520164 1104 7 -0.596836956438306 1104 8 -0.2671543592222534 1104 9 -0.1550216764343375 1104 10 -0.0725733436414373 1104 11 -0.09575034111303182 1104 12 -0.19123389993813333 1104 13 -0.2056323496055299 1104 14 -0.3018516677454528 1104 15 -0.12846829997408804 1104 16 -0.5187780921784864 1104 17 -0.5285962478625095 1104 18 -0.4510028828157715 1104 19 -1.5687312467367533 1104 20 -0.7044257680075248 1104 21 -0.5168669708959897 1104 22 -0.4462308602472902 1104 23 -0.24800806438805267 1104 24 1.0213137884690013 1104 25 0.3110997470598207 1105 1 -0.13204866912131763 1105 2 -0.21312280366030478 1105 3 0.37638750870564763 1105 4 -0.05694986802748403 1105 5 -0.05285890775654778 1105 6 -0.4073192579071083 1105 7 0.20134908676469365 1105 8 0.4150890192426431 1105 9 -0.546117875424212 1105 10 -0.04772450670785133 1105 11 -0.1589459876167254 1105 12 -0.13751232452258222 1105 13 -0.4104126592823434 1105 14 -0.2653614963087563 1105 15 -0.12074416367835283 1105 16 -0.43090670527217156 1105 17 -0.4527965656013535 1105 18 -0.48235350999429727 1105 19 -1.121264858289166 1105 20 -0.5385850642397045 1105 21 -0.3883854779976872 1105 22 -0.25053574726030803 1105 23 -0.6261682616706779 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1107 10 -0.005498723038598996 1107 11 -0.22114605643112384 1107 12 -0.088251173873374 1107 13 -0.273173203278283 1107 14 -0.17681728305655042 1107 15 -1.5778528723218024 1107 16 -0.4847675553111017 1107 17 -0.44433511072005416 1107 18 -0.39686972665100484 1107 19 0.4361000934502073 1107 20 0.40364705759800046 1107 21 0.3063094750093214 1107 22 0.012226607640904774 1107 23 -0.10779662769085263 1107 24 -4.3033662590867685 1107 25 -2.1818671270805616 1108 1 0.3907235683101824 1108 2 -0.10978356029346159 1108 3 0.4228251876255875 1108 4 0.4867423249224565 1108 5 0.5324532447312507 1108 6 -3.2871989433206394 1108 7 -1.3596254881818268 1108 8 0.11753930127182244 1108 9 0.029389129528603064 1108 10 0.5049466200244055 1108 11 0.19205529413110156 1108 12 0.302998052275548 1108 13 0.3343185532868276 1108 14 -0.06789417641218876 1108 15 -1.0860688656831456 1108 16 0.0750933929802784 1108 17 -0.17914787168898272 1108 18 0.0015252732831356752 1108 19 0.23240822009395146 1108 20 -1.330218421838417 1108 21 -0.6576652875665872 1108 22 -0.2445613286244992 1108 23 -0.265926783953887 1108 24 -0.8314949225307018 1108 25 0.05674683429054994 1109 1 0.7366824239483911 1109 2 0.6728753272387671 1109 3 -0.7448471609019727 1109 4 0.4923210940279965 1109 5 1.0146811871158263 1109 6 1.0927383210314048 1109 7 1.314954547019724 1109 8 1.5044105621810289 1109 9 1.0607752093452731 1109 10 0.8662403789979507 1109 11 0.7639945687729863 1109 12 0.5510121930323493 1109 13 0.7420519407433517 1109 14 0.9015487735593055 1109 15 -0.4306993406892943 1109 16 0.7179437090747314 1109 17 0.4795946877149717 1109 18 0.8546194766252455 1109 19 -0.9617490528705446 1109 20 0.42514906279714637 1109 21 0.634326091382718 1109 22 0.9355228415241967 1109 23 0.67403835813559 1109 24 -0.30337932227340403 1109 25 0.40966009006879034 1110 1 0.02583881877176708 1110 2 0.1822690160644136 1110 3 0.5746920964485653 1110 4 0.3764976479447597 1110 5 0.45240593376506055 1110 6 0.6956939197468621 1110 7 0.5535914205334294 1110 8 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-2.525512338511792 2216 12 -2.2038243598245195 2216 13 -1.789286762269363 2216 14 -2.1375776257031447 2216 15 -2.914193702110662 2216 16 -2.2095614664573056 2216 17 -2.4732108970838063 2216 18 -2.0831838137049736 2216 19 -1.406370340970664 2216 20 -2.2701849640678624 2216 21 -4.959475105893819 2216 22 -3.470095595620858 2216 23 -3.4901137370983513 2216 24 -3.4937745315060944 2216 25 -3.0567960253647826 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 41699.0 3 1.0 4 356.0 5 2019.0 6 2372.0 7 1826.0 8 1511.0 9 1371.0 10 1302.0 11 1205.0 12 1207.0 13 1153.0 14 1230.0 15 1266.0 16 1392.0 17 1502.0 18 1669.0 19 2085.0 20 2625.0 21 3299.0 22 4349.0 23 5204.0 24 5845.0 25 6752.0 26 7664.0 27 8377.0 28 9272.0 29 11034.0 30 12984.0 31 15897.0 32 19956.0 33 26494.0 34 36013.0 35 49916.0 36 71550.0 37 112225.0 38 133091.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.46088467619743 18.344607906985182 12.625324181775916 25.569183235041475 2 15.012924707196484 19.42412974461965 41.3635113027347 24.19943424544917 3 19.143404600954593 23.885883936529694 29.497447117689607 27.47326434482611 4 11.90914509845249 16.14940276437597 38.66059166998739 33.28086046718415 5 13.636969628141001 37.45582806232708 35.74820308475251 13.158999224779407 6 32.15240916759248 36.62774143109904 17.935728099411598 13.284121301896883 7 28.077208734258697 30.800475333806464 22.684975820693527 18.437340111241316 8 24.863147645413363 36.828425022072956 21.15885105083795 17.14957628167573 9 25.89145526183258 15.733325433425168 19.961422115134578 38.41379718960767 10 14.702686863964548 29.185282003838847 34.45118638822402 21.660844743972582 11 34.70478648120231 22.699414773512743 23.726358890637034 18.86943985464791 12 23.020447259106138 24.80991158831623 31.464322626939563 20.705318525638074 13 28.964198372024896 21.669085917982443 25.555193490732787 23.811522219259874 14 20.77967390427789 21.904402967020125 27.367584301191712 29.948338827510273 15 23.508385036946827 30.402383029146023 22.982242483090037 23.10698945081711 16 22.334652764633756 28.193586082605727 27.40077520017775 22.070985952582767 17 21.91988466077133 28.55896734523921 27.31192776814972 22.20922022583974 18 21.786742282734092 27.866912649288793 28.70344008123351 21.6429049867436 19 23.80608303061925 27.063544445889203 26.9456958089667 22.184676714524844 20 23.415117442595324 27.355105856330315 27.689363018748686 21.540413682325678 21 23.17903464438121 26.812818964141066 26.889345671962094 23.11880071951563 22 22.86661784189001 27.76067081951565 27.182837816709593 22.189873521884746 23 22.558182784709295 27.37162427934978 27.291145157016576 22.779047778924344 24 23.373105343712457 27.003275019337426 27.512137320408804 22.111482316541316 25 22.790386897267904 27.110128055112575 27.464949829583514 22.634535218036007 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 180.0 1 180.0 2 221.5 3 263.0 4 263.0 5 263.0 6 420.5 7 578.0 8 578.0 9 578.0 10 878.5 11 1179.0 12 1179.0 13 1179.0 14 1969.5 15 2760.0 16 2760.0 17 2760.0 18 4702.0 19 6644.0 20 6644.0 21 6644.0 22 10377.5 23 14111.0 24 14111.0 25 14111.0 26 20225.5 27 26340.0 28 26340.0 29 26340.0 30 34236.5 31 42133.0 32 42133.0 33 42133.0 34 48719.0 35 55305.0 36 55305.0 37 55305.0 38 60863.0 39 66421.0 40 66421.0 41 66421.0 42 70625.0 43 74829.0 44 74829.0 45 74829.0 46 77576.0 47 80323.0 48 80323.0 49 80323.0 50 78301.5 51 76280.0 52 76280.0 53 76280.0 54 68556.0 55 60832.0 56 60832.0 57 60832.0 58 52407.5 59 43983.0 60 43983.0 61 43983.0 62 36154.0 63 28325.0 64 28325.0 65 28325.0 66 21899.0 67 15473.0 68 15473.0 69 15473.0 70 11407.5 71 7342.0 72 7342.0 73 7342.0 74 5113.5 75 2885.0 76 2885.0 77 2885.0 78 1977.0 79 1069.0 80 1069.0 81 1069.0 82 698.0 83 327.0 84 327.0 85 327.0 86 212.0 87 97.0 88 97.0 89 97.0 90 61.0 91 25.0 92 25.0 93 25.0 94 16.5 95 8.0 96 8.0 97 8.0 98 4.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.19515791171161387 2 0.12110980018528482 3 0.1237426219284432 4 0.06828881396317012 5 0.023695395688425294 6 0.022708087534740906 7 0.2999771273611063 8 0.2909268026189994 9 0.018265200843161165 10 0.03932777478842809 11 0.013986865510528818 12 0.016619687253687183 13 0.014315968228423615 14 0.01645513589473979 15 0.013657762792634025 16 0.02106257394526693 17 0.018758854920003357 18 0.013493211433686625 19 0.02534090927789927 20 0.015961481817897593 21 0.013493211433686625 22 0.01612603317684499 23 0.016784238612634582 24 0.013657762792634025 25 0.013822314151581422 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 607713.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 88.04955870665496 #Duplication Level Percentage of deduplicated Percentage of total 1 93.86885211941095 82.65111005414387 2 4.639623545657471 8.170336115202922 3 0.7808079087982948 2.0624937541306787 4 0.2654949772614083 0.9350686234680159 5 0.12273401567276729 0.5403337959141417 6 0.07190504976499625 0.37987247403527913 7 0.050342673324854724 0.31028551192567244 8 0.035742789201247446 0.2517709452891864 9 0.025694485309925356 0.20361492834602007 >10 0.12066732803237576 1.9213269100854111 >50 0.008787143494557665 0.5694124598041916 >100 0.00841316766431259 1.4927453441470437 >500 9.347964070088662E-4 0.5116290835076065 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 721 0.11864152980107386 No Hit GTATCAACGCAGAGTACTTTTTTTT 673 0.11074306457159876 No Hit GTCCTACAGTGTGCATTTCTCATTT 614 0.10103453439370229 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGACC 190 0.0 14.033803 4 GCTCTAA 55 1.8906764E-4 13.874482 1 GTCTTAC 55 1.8906764E-4 13.874482 1 GTATTAT 85 2.5598638E-7 13.46641 1 TATAGGA 80 1.918881E-6 13.107039 2 AGGACGT 285 0.0 13.023852 5 CTTTAGG 225 0.0 12.2943325 1 GGACGTG 295 0.0 12.259741 6 AGGACCT 280 0.0 12.236697 5 TGATACT 55 0.003009864 12.120103 4 TTAGGAC 205 0.0 12.089864 3 TCCTACA 590 0.0 11.955882 2 GTCCTAG 80 2.7430087E-5 11.923383 1 TAGGACG 320 0.0 11.60608 4 TGTAGGA 505 0.0 11.514376 2 TCTATAC 75 2.0053987E-4 11.438872 3 GCATTTC 365 0.0 11.430574 13 GACGTGA 150 1.6916601E-10 11.4209175 7 GTCCTAC 545 0.0 11.376439 1 GTAGGAC 480 0.0 11.319717 3 >>END_MODULE