FastQCFastQC Report
Thu 26 May 2016
SRR1926899_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926899_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences607713
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT12930.21276490711898544No Hit
GGTATCAACGCAGAGTACTTTTTTT8400.13822314151581422No Hit
GTCCTACAGTGGACATTTCTAAATT8300.13657762792634023No Hit
CTGTAGGACGTGGAATATGGCAAGA7210.11864152980107386No Hit
TATCAACGCAGAGTACTTTTTTTTT6880.11321133495580972No Hit
GTCCTACAGTGTGCATTTCTCATTT6390.10514831836738724No Hit
GTCCTAAAGTGTGTATTTCTCATTT6160.10136363711159709No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG807.830749E-915.4154418
CCAACGA602.597982E-514.22963719
GGCCTAG551.8981504E-413.8680381
TAGGACC2500.012.9526914
GTCCTAA2100.012.7123691
TAGACAG853.8225535E-612.3252255
TGTAGGA4500.012.286592
GTACTAG701.0527774E-412.2583551
ACTATCC550.003095840312.0746248
CGTTTCC550.003095840312.07462414
CTGTAGG4700.011.7657021
GTAGGAC4300.011.741843
CTCCTAA657.772551E-411.7344931
TGAGTTA1354.800313E-911.24409412
GTAGAAC1451.1550583E-911.168883
GTATTAA600.005713379511.1233221
ATCCCGT600.00592421811.06931710
TCCTAAA2500.011.0579322
AACCTGT951.318823E-511.0278336
CCTAAAG2600.010.9919993
Produced by FastQC (version 0.11.5)