FastQCFastQC Report
Thu 26 May 2016
SRR1926898_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926898_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences683934
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT10620.15527814087324215No Hit
GTACATGGGAAGCAGTGGTATCAAC7100.10381118645951218No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTATAC851.6392733E-814.5766283
GGATAAC400.00513760514.3068221
CTTCGTG551.9842909E-413.7961589
CCGTGCA551.9842909E-413.7961589
TAGGACT707.078841E-613.6025324
GTCCTAC2900.012.1690211
TAGGTAG550.003018269512.1159515
CTGCGTT1353.8198777E-1011.94040211
TGCGTTG1353.8198777E-1011.94040212
TTAGGAC1459.094947E-1111.8314273
TTCTATA1052.596771E-711.8009932
CGTTGAT1451.0004442E-1011.77086314
GCCTTAT657.750107E-411.7389321
GTTATGT657.750107E-411.7389321
TAGGACC1156.8263034E-811.5917234
GTCCCGG751.995404E-411.4454571
CCTACAG3550.011.275963
TCCTACA3300.011.2645852
GACCTAC600.00569855311.1275291
TCTGCGT1451.2423698E-911.12017810
Produced by FastQC (version 0.11.5)